A sedimented sample of a 59 kDa dodecameric helicase yields high-resolution solid-state NMR spectra. - ECOFECT - Dynamiques éco-évolutives des maladies infectieuses
Article Dans Une Revue Angewandte Chemie International Edition Année : 2012

A sedimented sample of a 59 kDa dodecameric helicase yields high-resolution solid-state NMR spectra.

Résumé

Crystal clear: Preparing solid-state NMR samples that yield high-resolution spectra displaying high sensitivity is time-consuming and complicated. A sample of the 59 kDa protein DnaB, prepared simply by preparative centrifugation, provides spectra that are as good as the ones from carefully grown microcrystals.
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Dates et versions

hal-00965867 , version 1 (23-11-2020)

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Carole Gardiennet, Anne K Schütz, Andreas Hunkeler, Britta Kunert, Laurent Terradot, et al.. A sedimented sample of a 59 kDa dodecameric helicase yields high-resolution solid-state NMR spectra.. Angewandte Chemie International Edition, 2012, 51 (31), pp.7855-8. ⟨10.1002/anie.201200779⟩. ⟨hal-00965867⟩
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