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Purification and anti-inflammatory and antioxidant properties of phenolic fractions from co-products of production of oleoproteaginous protein isolates

Abstract : apeseed and sunflower are the most cultivated oilseed plants in Europe in general, and in France in particular. Some industrialists are currently focusing on the development of industrial processes for the extraction/purification of proteins from the oil cakes of these two plants. These processes generate co-products which are saline aqueous effluents rich in phenolic compounds such as cholorgenic acid (CGA, for sunflower) and sinapine (SP, for rapeseed). The capture of these phenolic compounds, which can act as natural antioxidants and/or anti-inflammatory agents in nutrition and health, is therefore a promising way of valorization. The main objectives of this work were: 1) to characterize and identify the phenolic compounds of protein isolate by-products from SFM and RSM; 2) to select the best macroporous resins and to study the adsorption mechanism of phenolic compounds; 3) to optimize the conditions in the phenolic compounds adsorption column; and 4) to evaluate the biological activities of the obtained phenolic fractions, especially the antioxidant and anti-inflammatory properties.By different analytical methods, we determined that the liquid effluents consisted of phenolic compounds, amino acids, carbohydrates, and salt, which have a low molecular weight and can easily pass through a UF/DF membrane. All phenolic compounds were identified by HPLC and HPLC-ESI-MS analysis in comparison with standards. CGA is the main phenolic compound in the sunflower effluent. The main phenolic compound of rapeseed effluents is MS. Unlike sunflower, they also contain many other minor compounds. The adsorption/desorption of sunflower and rapeseed phenolic compounds was evaluated using different macroporous resins including XAD4, XAD7, XAD16, XAD1180 and HP20. We found that all phenolic compounds adsorbed readily onto the resins. XAD7 and XAD16 resins showed the best adsorption/desorption properties in sunflower and rapeseed liquid effluents, respectively. The results showed that the adsorption of all phenolic compounds follows a Langmuir model. According to the determined thermodynamic parameters, the adsorption process, is in all cases physical and is exothermic.The optimal condition for column adsorption was determined on the selected resins by experimental planning and multicriteria optimization. A multicriteria optimization methodology based on design of experiments showed the optimal conditions were adsorption flow rate of 15 BV/h at pH 2.7 for CGA from SFM. Meanwhile, adsorption flow rate of 13.3 BV/h and at pH ranging from 2 to 5 were the optimal conditions for sinapine from RSM. Ethanol solutions 50% (v/v) for chlorogenic acid, 70% (v/v) for sinapine were used for desorption.These approaches successfully produced the phenolic fractions for biological activities such as antioxidation and anti-inflammation. Phenolic fraction showed a higher antioxidant capacity than vitamin C in DPPH and ABTS assays (IC50/phenolic fractions < IC50 vitamin C, p < 0.05. In addition, it was discussed whether the phenolic fractions obtained in this project also showed an inflammatory effect. The sunflower fraction (CGA) effectively inhibited the production of TNF-α, which is a pro-inflammatory marker when a sample is treated with LPS. However, the rapeseed fractions were not effective against proinflammatory mediators. None of the fractions showed cytotoxicity.
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Submitted on : Wednesday, June 1, 2022 - 9:58:11 AM
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Thi Tuong Le. Purification and anti-inflammatory and antioxidant properties of phenolic fractions from co-products of production of oleoproteaginous protein isolates. Chemical and Process Engineering. Université de Lorraine, 2021. English. ⟨NNT : 2021LORR0270⟩. ⟨tel-03683992⟩

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