Fractionation of yeast extract by nanofiltration process to assess key compounds involved in CHO cell culture improvement

Abstract : Yeast extract (YE) is known to greatly enhance mammalian cell culture performances, but its undefined composition decreases process reliability. Accordingly, in the present study, the nature of YE compounds involved in the improvement of recombinant CHO cell growth and IgG production was investigated. First, the benefits of YE were verified, revealing that it increased maximal concentrations of viable cells and IgG up to 73 and 60%, respectively compared to a reference culture. Then, the analyses of YE composition highlighted the presence of molecules such as amino acids, vitamins, salts, nucleobase, and glucose that were contained in reference medium, while others including peptides, trehalose, polysaccharides, and nucleic acids were not. Consequently, YE was fractionated by a nanofiltration process to deeper evaluate its effects on CHO cell cultures. The YE molecules already contained in reference medium were mainly isolated in the permeate fraction together with trehalose and short peptides, while other molecules were concentrated in the retentate. Permeate, which was free of macromolecules, exhibited a similar positive effect than raw YE on maximal concentrations. Additional studies on cell energetic metabolism underlined that dipeptides and tripeptides in permeate were used as an efficient source of nitrogenous substrates.
Type de document :
Article dans une revue
Biotechnology Progress, Wiley, 2015, 31 (4), pp.875-882. 〈10.1002/btpr.2110〉
Domaine :
Liste complète des métadonnées

https://hal.univ-lorraine.fr/hal-01267003
Contributeur : Lrgp Ul <>
Soumis le : mercredi 3 février 2016 - 16:41:17
Dernière modification le : jeudi 18 janvier 2018 - 16:02:03

Lien texte intégral

Identifiants

Collections

Citation

Mathilde Mosser, Romain Kapel, Isabelle Chevalot, Eric Olmos, Ivan Marc, et al.. Fractionation of yeast extract by nanofiltration process to assess key compounds involved in CHO cell culture improvement. Biotechnology Progress, Wiley, 2015, 31 (4), pp.875-882. 〈10.1002/btpr.2110〉. 〈hal-01267003〉

Partager

Métriques

Consultations de la notice

58