Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis

Abstract : This study investigated the effect of Angelica sinensis polysaccharides (APS-3c) on rat osteoarthritis (OA) model in vivo and rat interleukin-1-beta-(IL-1 beta-) stimulated chondrocytes in vitro. APS-3c was administrated into rat OA knee joints and had protective effects on rat OA cartilage in vivo. Primary rat articular chondrocytes were cotreated with APS-3c and IL-1 beta in vitro. 2 similar to 50 mu g/mL APS-3c had no effect on chondrocytes viability, whereas it increased the proteoglycans (PGs) synthesis inhibited by IL-1 beta. Microarray analysis showed that the significant changes were concentrated in the genes which were involved in PGs synthesis. RT-PCR confirmed that treatment with APS-3c increased the mRNA expression of aggrecan and glycosyltransferases (GTs) inhibited by IL-1 beta but did not affect the mRNA expression of matrix-degrading enzymes. These results indicate that APS-3c can improve PGs synthesis of chondrocytes on rat OA model in vivo and IL-1 beta-stimulated chondrocytes in vitro, which is due to the promotion of the expression of aggrecan and GTs involved in PGs synthesis but not the inhibition of the expression of matrix-degrading enzymes. Our findings suggest the clinical relevance of APS-3c in the prospective of future alternative medical treatment for OA.
Type de document :
Article dans une revue
Evidence-Based Complementary and Alternative Medicine, Hindawi Publishing Corporation, 2013, 2013, pp.794761. 〈https://www.hindawi.com/journals/ecam/2013/794761/〉. 〈10.1155/2013/794761〉
Liste complète des métadonnées

https://hal.univ-lorraine.fr/hal-01453064
Contributeur : Imopa Ul <>
Soumis le : jeudi 2 février 2017 - 15:07:02
Dernière modification le : mardi 16 janvier 2018 - 09:50:02
Document(s) archivé(s) le : vendredi 5 mai 2017 - 11:41:10

Fichier

794761.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Jun Qin, Yan-Song Liu, Jun Liu, Jing Li, Yang Tan, et al.. Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis. Evidence-Based Complementary and Alternative Medicine, Hindawi Publishing Corporation, 2013, 2013, pp.794761. 〈https://www.hindawi.com/journals/ecam/2013/794761/〉. 〈10.1155/2013/794761〉. 〈hal-01453064〉

Partager

Métriques

Consultations de la notice

56

Téléchargements de fichiers

42