Coumarin and Furanocoumarin Quantitation in Citrus Peel via Ultraperformance Liquid Chromatography Coupled with Mass Spectrometry (UPLC-MS)

Abstract : Coumarins and furanocoumarins are secondary metabolites commonly found in citrus plants. These molecules are allelochemical compounds in plants that have controversial effects on humans, such as phototoxicity and the commonly described interactions with drugs, referred to as the ``grapefruit juice effect''. Thus, it is important to develop a reliable method to identify and quantitate the coumarins and furanocoumarins in citrus extracts. For this purpose, we herein describe an ultraperformance liquid chromatography coupled with mass spectrometry (UPLC-MS)-based method. We first developed a rapid UPLC method (20 min) to separate the isomers of each furanocoumarin. A subsequent single ion monitoring MS detection method was performed to distinguish between the molecules, which were possibly coeluting but had different molecular weights. The method was successfully used to separate and quantitate 6 coumarins and 21 furanocoumarins in variable amounts within peel extracts (flayed and albedo) of 6 varieties of Citrus (sweet orange, lemon, grapefruit, bergamot, pummelo, and clementine). This method combines high selectivity and sensitivity in a rapid analysis and is useful for fingerprinting Citrus species via their coumarin and furanocoumarin contents.
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Article dans une revue
Journal of Agricultural and Food Chemistry, American Chemical Society, 2013, 61 (45), pp.10677-10684. 〈10.1021/jf402763t〉
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Soumis le : jeudi 23 février 2017 - 10:44:35
Dernière modification le : vendredi 19 octobre 2018 - 15:22:02

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Audray Dugrand, Alexandre Olry, Thibault Duval, Alain Hehn, Yann Froelicher, et al.. Coumarin and Furanocoumarin Quantitation in Citrus Peel via Ultraperformance Liquid Chromatography Coupled with Mass Spectrometry (UPLC-MS). Journal of Agricultural and Food Chemistry, American Chemical Society, 2013, 61 (45), pp.10677-10684. 〈10.1021/jf402763t〉. 〈hal-01474772〉

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