Skip to Main content Skip to Navigation
Journal articles

Coiled-coil protein Scy is a key component of a multiprotein assembly controlling polarized growth in Streptomyces

Abstract : Polarized growth in eukaryotes requires polar multiprotein complexes. Here, we establish that selection and maintenance of cell polarity for growth also requires a dedicated multiprotein assembly in the filamentous bacterium, Streptomyces coelicolor. We present evidence for a tip organizing center and confirm two of its main components: Scy (Streptomyces cytoskeletal element), a unique bacterial coiled-coil protein with an unusual repeat periodicity, and the known polarity determinant DivIVA. We also establish a link between the tip organizing center and the filament-forming protein FilP. Interestingly, both deletion and overproduction of Scy generated multiple polarity centers, suggesting a mechanism wherein Scy can both promote and limit the number of emerging polarity centers via the organization of the Scy-DivIVA assemblies. We propose that Scy is a molecular "assembler," which, by sequestering DivIVA, promotes the establishment of new polarity centers for de novo tip formation during branching, as well as supporting polarized growth at existing hyphal tips.
Document type :
Journal articles
Complete list of metadata

Cited literature [44 references]  Display  Hide  Download
Contributor : Dynamic Ul Connect in order to contact the contributor
Submitted on : Monday, February 27, 2017 - 11:31:14 AM
Last modification on : Thursday, January 7, 2021 - 3:43:15 PM
Long-term archiving on: : Sunday, May 28, 2017 - 12:56:11 PM


Publisher files allowed on an open archive




A. Holmes, J. Walshaw, R. M. Leggett, A. Thibessard, K. A. Dalton, et al.. Coiled-coil protein Scy is a key component of a multiprotein assembly controlling polarized growth in Streptomyces. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2013, 110 (5), pp.E397 - E406. ⟨10.1073/pnas.1210657110⟩. ⟨hal-01477256⟩



Record views


Files downloads