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Coiled-coil protein Scy is a key component of a multiprotein assembly controlling polarized growth in Streptomyces

Abstract : Polarized growth in eukaryotes requires polar multiprotein complexes. Here, we establish that selection and maintenance of cell polarity for growth also requires a dedicated multiprotein assembly in the filamentous bacterium, Streptomyces coelicolor. We present evidence for a tip organizing center and confirm two of its main components: Scy (Streptomyces cytoskeletal element), a unique bacterial coiled-coil protein with an unusual repeat periodicity, and the known polarity determinant DivIVA. We also establish a link between the tip organizing center and the filament-forming protein FilP. Interestingly, both deletion and overproduction of Scy generated multiple polarity centers, suggesting a mechanism wherein Scy can both promote and limit the number of emerging polarity centers via the organization of the Scy-DivIVA assemblies. We propose that Scy is a molecular "assembler," which, by sequestering DivIVA, promotes the establishment of new polarity centers for de novo tip formation during branching, as well as supporting polarized growth at existing hyphal tips.
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A. Holmes, J. Walshaw, R. M. Leggett, A. Thibessard, K. A. Dalton, et al.. Coiled-coil protein Scy is a key component of a multiprotein assembly controlling polarized growth in Streptomyces. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2013, 110 (5), pp.E397 - E406. ⟨10.1073/pnas.1210657110⟩. ⟨hal-01477256⟩

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