Hind limb unloading, a model of spaceflight conditions, leads to decreased B lymphopoiesis similar to aging

Abstract : Within the bone marrow, the endosteal niche plays a crucial role in B-cell differentiation. Because spaceflight is associated with osteoporosis, we investigated whether changes in bone microstructure induced by a ground-based model of spaceflight, hind limb unloading (HU), could affect B lymphopoiesis. To this end, we analyzed both bone parameters and the frequency of early hematopoietic precursors and cells of theBlineage after 3, 6, 13, and 21 d of HU. We found that limb disuse leads to a decrease in both bone microstructure and the frequency of B-cell progenitors in the bone marrow. Although multi-potent hematopoietic progenitors were not affected by HU, a decrease in B lymphopoiesis was observed as of the common lymphoid progenitor (CLP) stage with a major block at the progenitor B (pro-B) to precursor B (pre-B) cell transition (5-to 10-fold decrease). Themodifications in B lymphopoiesis were similar to those observed in aged mice and, as with aging, decreased B-cell generation in HU mice was associated with reduced expression of B-cell transcription factors, early B-cell factor (EBF) and Pax5, and an alteration in STAT5-mediated IL-7 signaling. These findings demonstrate that mechanical unloading of hind limbs results in a decrease in early B-cell differentiation resembling age-related modifications in B lymphopoiesis.
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FASEB Journal, Federation of American Society of Experimental Biology, 2015, 29 (2), pp. 455-463. 〈http://www.fasebj.org/content/29/2/455〉. 〈10.1096/fj.14-259770 〉
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https://hal.univ-lorraine.fr/hal-01481935
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Soumis le : vendredi 3 mars 2017 - 09:43:40
Dernière modification le : jeudi 20 septembre 2018 - 15:50:11

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Chloé Lescale, Véronique Schenten, Dounia Djeghloul, Meriem Bennabi, Fanny Gaignier, et al.. Hind limb unloading, a model of spaceflight conditions, leads to decreased B lymphopoiesis similar to aging. FASEB Journal, Federation of American Society of Experimental Biology, 2015, 29 (2), pp. 455-463. 〈http://www.fasebj.org/content/29/2/455〉. 〈10.1096/fj.14-259770 〉. 〈hal-01481935〉

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