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Magnetic non-equivalence and dynamic NMR of N-methylene protons in a Histamine-containing pseudopeptide: Alanyl-Glycyl-Histamine

Abstract : The objective of the present research is to explore the potential of an additional feature of proton 1D-NMR for the study of the properties of peptides in aqueous solution, namely the magnetic non-equivalence of N-methylene protons in linear acyclic peptides induced by an asymmetric carbon atom located nearby. For this exploratory study, we have chosen an L-Alanyl terminal residue adjacent to a Glycyl residue, itself grafted to an optically inactive terminal Histamine unit, i.e., the Histamine-containing pseudopeptide L-Alanyl-Glycyl-Histamine (AGHA). The pH- and temperature-dependence of magnetic non-equivalences of Glycyl and Histamine N-methylene protons was modeled as a weighted mean over four ionization states BH3, BH2, BH, B of AGHA molecules found in the pH range explored: pH -0.78 to 12. In parallel, this research has made it possible to assess the pK's governing the speciation curve, the base- and acid-catalyzed proton exchange rates and the pK's of amide bonds.
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https://hal.univ-lorraine.fr/hal-01495975
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Submitted on : Monday, March 27, 2017 - 10:23:56 AM
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Bernard Henry, Patrick Gizzi, Jean-Jacques Delpuech. Magnetic non-equivalence and dynamic NMR of N-methylene protons in a Histamine-containing pseudopeptide: Alanyl-Glycyl-Histamine. Tetrahedron, Elsevier, 2015, 71 (36), pp.6227-6244. ⟨10.1016/j.tet.2015.06.079⟩. ⟨hal-01495975⟩

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