Skip to Main content Skip to Navigation
Journal articles

Characterization and distribution of two insertion sequences, IS1191 and iso-IS981, in Streptococcus thermophilus: does intergeneric transfer of insertion sequences occur in lactic acid bacteria co-cultures?

Abstract : A chromosomal repeated sequence from Streptococcus thermophilus was identified as a new insertion sequence (IS), IS1191. This is the first IS element characterized in this species. This 1313 bp element has 28 bp imperfect terminal inverted repeats and is flanked by short direct repeats of 8 bp. The single large open reading frame of IS1191 encodes a 391-amino-acid protein which displays homologies with transposases encodes by IS1201 from Lactobacillus helveticus (44.5% amino-acid sequence identity) and by the other ISs of the IS256 family. One of the copies of IS1191 is inserted into a truncated iso-IS981 element. The nucleotide sequences of two truncated iso-IS981s from S. thermophilus and the sequence of IS981 element from Lactococcus lactis share more than 99% identity. The distribution of these insertion sequences in L. lactis and S. thermophilus strains suggests that intergeneric transfers occur during cocultures used in the manufacture of cheese.
Document type :
Journal articles
Complete list of metadata

https://hal.univ-lorraine.fr/hal-01659595
Contributor : Pierre Leblond <>
Submitted on : Friday, December 8, 2017 - 3:23:50 PM
Last modification on : Monday, October 19, 2020 - 6:34:03 PM

Identifiers

Collections

Citation

Gérard Guedon, Florence Bourgoin, M Pébay, Y Roussel, C Colmin, et al.. Characterization and distribution of two insertion sequences, IS1191 and iso-IS981, in Streptococcus thermophilus: does intergeneric transfer of insertion sequences occur in lactic acid bacteria co-cultures?. Molecular Microbiology, Wiley, 1995, 16 (1), pp.69-78. ⟨10.1111/j.1365-2958.1995.tb02392.x⟩. ⟨hal-01659595⟩

Share

Metrics

Record views

155