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Master thesis

Développement de nouveaux immunoconjugués pour l'activation directe du complément à la surface de cellules infectées par le VIH

Abstract : One of the main barriers to HIV eradication is the presence of latent viral reservoirs in infected patients. In this context, the LIH is working on a “shock and kill” strategy by targeting latent cells infected by HIV with heteromultimerrs able to activate the MAC to their surface and induce their cell lysis. In the current work, this innovative immunotherapy has been tested on FLYgp120 cells with multimers expressing a scFv antigp120 antibody and the positive regulator of C3b, complement factor H-related protein 4 (FHR4) . Multimers have been produced by transfection of HEK293 cells and purified by affinity chromatography on column HIStrap. The tests performed pointed out the potency of multimers to induce a C3b deposit and form C5b9, a critical component of CDC, to the surface of flygp120 cells. High valency FHR4 multimers production remains now to be improved in order to obtain an efficient CDC in cellular models of latency
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/public/BUS_M_2017_BERTRAND_EVEN.pdf
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https://hal.univ-lorraine.fr/hal-01662589
Contributor : Memoires Ul <>
Submitted on : Wednesday, December 13, 2017 - 11:54:03 AM
Last modification on : Monday, October 19, 2020 - 2:41:35 PM

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  • HAL Id : hal-01662589, version 1

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Citation

Even Bertrand. Développement de nouveaux immunoconjugués pour l'activation directe du complément à la surface de cellules infectées par le VIH. Microbiologie et Parasitologie. 2017. ⟨hal-01662589⟩

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