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Article Dans Une Revue Bio-Medical Materials and Engineering Année : 2017

Transforming growth factor-beta 1 or ascorbic acid are able to differentiate Wharton’s jelly mesenchymal stem cells towards a smooth muscle phenotype

Résumé

Wharton's jelly mesenchymal stem cells (WJ-MSCs) are widely used in tissue engineering. In vascular engineering, the ability to obtain a vessel replacement with contractile smooth muscle cells (SMC) is a key factor. In this work, we demonstrated that WJ-MSCs differentiate towards a SMC phenotype with various stimulations in vitro and that the modification of redox state could be involved. WJ-MSCs were isolated from umbilical cords. After their expansion, the cells were stimulated with ascorbic acid (AA, 300 μM) or transforming growth factor (TGF)-β1 (1 to 5 ng/mL). SMC markers were analyzed by Western blot. Modification of redox state was evaluated by reactive oxygen species (ROS) production and glutathione (GSH) content measurements. TGF-β1 or AA-stimulated WJ-MSCs express early and intermediate SMC markers. TGF-β1 (5 ng/mL) modifies the redox state by a ROS production and a GSH content drop, while AA has no effect. This work showed that TGF-β1 and AA are effective SMC phenotype inducers to differentiate WJ-MSCs.
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Dates et versions

hal-01691343 , version 1 (23-01-2018)

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Citer

B. Mesure, S. Huber-Villaume, P. Menu, E. Velot. Transforming growth factor-beta 1 or ascorbic acid are able to differentiate Wharton’s jelly mesenchymal stem cells towards a smooth muscle phenotype. Bio-Medical Materials and Engineering, 2017, 28 (s1), pp.S101 - S105. ⟨10.3233/BME-171630⟩. ⟨hal-01691343⟩
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