Skip to Main content Skip to Navigation
Journal articles

Transforming growth factor-beta 1 or ascorbic acid are able to differentiate Wharton’s jelly mesenchymal stem cells towards a smooth muscle phenotype

Abstract : Wharton's jelly mesenchymal stem cells (WJ-MSCs) are widely used in tissue engineering. In vascular engineering, the ability to obtain a vessel replacement with contractile smooth muscle cells (SMC) is a key factor. In this work, we demonstrated that WJ-MSCs differentiate towards a SMC phenotype with various stimulations in vitro and that the modification of redox state could be involved. WJ-MSCs were isolated from umbilical cords. After their expansion, the cells were stimulated with ascorbic acid (AA, 300 μM) or transforming growth factor (TGF)-β1 (1 to 5 ng/mL). SMC markers were analyzed by Western blot. Modification of redox state was evaluated by reactive oxygen species (ROS) production and glutathione (GSH) content measurements. TGF-β1 or AA-stimulated WJ-MSCs express early and intermediate SMC markers. TGF-β1 (5 ng/mL) modifies the redox state by a ROS production and a GSH content drop, while AA has no effect. This work showed that TGF-β1 and AA are effective SMC phenotype inducers to differentiate WJ-MSCs.
Document type :
Journal articles
Complete list of metadatas

https://hal.univ-lorraine.fr/hal-01691343
Contributor : Émilie Velot <>
Submitted on : Tuesday, January 23, 2018 - 7:35:59 PM
Last modification on : Tuesday, October 13, 2020 - 10:46:34 AM

Identifiers

Collections

Citation

B. Mesure, S. Huber-Villaume, P. Menu, E. Velot. Transforming growth factor-beta 1 or ascorbic acid are able to differentiate Wharton’s jelly mesenchymal stem cells towards a smooth muscle phenotype. Bio-Medical Materials and Engineering, IOS Press, 2017, 28 (s1), pp.S101 - S105. ⟨10.3233/BME-171630⟩. ⟨hal-01691343⟩

Share

Metrics

Record views

105