A. Les, 5 ug) d'oligodT (Promega) Les oligodT se lient à la queue polyA en 3' des ARNm pour permettre l'élongation, le volume étant ajusté à 32, ul avec de l'eau stérile (sans Rnase ), le tout est incubé pendant 5 min à 65°C

L. Puis, Promega) ainsi que les dNTP (O,2/lM de chaque dNTP) (Promega) sont ajoutés. L'enzyme de reverse transcription, unités) (Promega) est ajoutée en demier. Le volume final est de SO/l!. Le tout est incubé 2 heures à 37°C. L'activité de l'enzyme est arrêtée par 2 min à 90°C

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