. La-dénaturation-de-l, ADN à la chaleur (95°C, 2 minutes) pour séparer les deux brins

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«. Bbk, de sorte que l'on retrouve sur la feuille d'examen la mention BBK -ou BBK +

U. Babinski-positif-signe-une-atteinte-du-tronc-cérébral and /. Ou-une-atteinte-de-la-voie-pyramidale, Cependant, chez l'enfant, le Babinski peut être positif jusqu'à l'âge d'un an. Cette réaction est physiologique et témoigne de l'immaturité relative du système nerveux encore en développement de l'enfant

. La-seconde-limite-est-celle-du-nombre-de-copies-de-la-cible, Des amplifications à partir d'une seule copie peuvent être réalisées mais les problèmes de contamination rendent cela difficiles

P. Lors-d-'une, il peut apparaître des amplifications artéfactuelles résultant de la contamination par les produits des amplifications précédentes Il est impossible de s'en affranchir complètement mais certaines précautions peuvent être prises telles que réaliser les incubations dans un laboratoire différent ; utiliser des cônes de prélèvement munis de coton protecteur ou de piston ; irradier les amorces avec des rayons ultraviolets ; fractionner en petits volumes tous les composants des incubations

. La-quantité-d, amplicons est corrélée à la quantité initiale d'ADN de la matière originale, ce qui permet pour d