E. V. Koonin, Pseudouridine Synthases: Four Families of Enzymes Containing a Putative Uridine-Binding Motif Also Conserved in dUTPases and dCTP Deaminases, Nucleic Acids Research, vol.148, issue.1, pp.2411-2415, 1996.
DOI : 10.1007/BF00362080

J. Ofengand, Ribosomal RNA pseudouridines and pseudouridine synthases, FEBS Letters, vol.28, issue.1, pp.17-25, 2002.
DOI : 10.1093/nar/28.12.2342

URL : http://onlinelibrary.wiley.com/doi/10.1016/S0014-5793(02)02305-0/pdf

Y. Motorin, G. Keith, C. Simon, D. Foiret, G. Simos et al., The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity, RNA, vol.4, issue.7, pp.856-869, 1998.
DOI : 10.1017/S1355838298980396

S. Massenet, Y. Motorin, D. L. Lafontaine, E. C. Hurt, H. Grosjean et al., Spliceosomal U Small Nuclear RNAs (snRNAs) Reveals that Pseudouridine Synthase Pus1p Exhibits a Dual Substrate Specificity for U2 snRNA and tRNA, Molecular and Cellular Biology, vol.19, issue.3, pp.2142-2154, 1999.
DOI : 10.1128/MCB.19.3.2142

F. Lecointe, G. Simos, A. Sauer, E. C. Hurt, Y. Motorin et al., in tRNA Anticodon Loop, Journal of Biological Chemistry, vol.238, issue.3, pp.1316-1323, 1998.
DOI : 10.1016/0168-9525(93)90207-X

H. F. Becker, Y. Motorin, R. J. Planta, and H. Grosjean, The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of psi55 in both mitochondrial and cytoplasmic tRNAs, Nucleic Acids Research, vol.25, issue.22, pp.4493-4499, 1997.
DOI : 10.1093/nar/25.22.4493

D. L. Lafontaine, C. Bousquet-antonelli, Y. Henry, M. Caizergues-ferrer, and D. Tollervey, The box H+ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase, Genes & Development, vol.12, issue.4, pp.527-537, 1998.
DOI : 10.1101/gad.12.4.527

N. J. Watkins, A. Gottschalk, G. Neubauer, B. Kastner, P. Fabrizio et al., Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure, RNA, vol.4, issue.12, pp.1549-1568, 1998.
DOI : 10.1017/S1355838298980761

I. Ansmant, S. Massenet, H. Grosjean, Y. Motorin, and C. Branlant, Identification of the Saccharomyces cerevisiae RNA:pseudouridine synthase responsible for formation of ??2819 in 21S mitochondrial ribosomal RNA, Nucleic Acids Research, vol.4, issue.5, pp.1941-1946, 2000.
DOI : 10.1016/S1097-2765(00)80395-0

X. Ma, X. Zhao, Y. , and Y. T. , Pseudouridylation (Psi) of U2 snRNA in S.cerevisiae is catalyzed by an RNA-independent mechanism, The EMBO Journal, vol.22, issue.8, pp.1889-1897, 2003.
DOI : 10.1093/emboj/cdg191

I. Behm-ansmant, A. Urban, X. Ma, Y. T. Yu, Y. Motorin et al., The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:??-synthase also acting on tRNAs, RNA, vol.9, issue.11, pp.1371-1382, 2003.
DOI : 10.1261/rna.5520403

O. Oltmanns, A. Bacher, F. Lingens, and F. K. Zimmermann, Biochemical and genetic classification of riboflavine deficient mutants of Saccharomyces cerevisiae, MGG Molecular & General Genetics, vol.22, issue.4, pp.306-313, 1969.
DOI : 10.1007/BF00277585

S. Sprinzl, C. Horn, M. Brown, A. Ioudovitch, and S. Steinberg, Compilation of tRNA sequences and sequences of tRNA genes, Nucleic Acids Research, vol.26, issue.1, pp.148-153, 1998.
DOI : 10.1093/nar/26.1.148

URL : https://hal.archives-ouvertes.fr/hal-00356160

J. G. Arnez and T. A. Steitz, Crystal Structure of Unmodified tRNAGln Complexed with Glutaminyl-tRNA Synthetase and ATP Suggests a Possible Role for Pseudo-Uridines in Stabilization of RNA Structure, Biochemistry, vol.33, issue.24, pp.7560-7567, 1994.
DOI : 10.1021/bi00190a008

P. Auffinger and E. Westhof, RNA hydration: three nanoseconds of multiple molecular dynamics simulations of the solvated tRNA Asp anticodon hairpin 1 1Edited by J. Karn, Journal of Molecular Biology, vol.269, issue.3, pp.326-341, 1997.
DOI : 10.1006/jmbi.1997.1022

D. Mumberg, R. Muller, and M. Funk, Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds, Gene, vol.156, issue.1, pp.119-122, 1995.
DOI : 10.1016/0378-1119(95)00037-7

D. Gietz, . St, A. Jean, R. A. Woods, and R. H. Schiestl, Improved method for high efficiency transformation of intact yeast cells, Nucleic Acids Research, vol.20, issue.6, p.1425, 1992.
DOI : 10.1093/nar/20.6.1425

A. Urban, I. Ansmant, and Y. Motorin, Optimisation of expression and purification of the recombinant Yol066 (Rib2) protein from Saccharomyces cerevisiae, Journal of Chromatography B, vol.786, issue.1-2, pp.187-195, 2003.
DOI : 10.1016/S1570-0232(02)00742-0

H. Jiang, Y. A. Motorin, Y. Jin, and H. Grosjean, Pleiotropic effects of intron removal on base modification pattern of yeast tRNAPhe: an in vitro study, Nucleic Acids Research, vol.25, issue.14, pp.2694-2701, 1997.
DOI : 10.1093/nar/25.14.2694

A. Bakin, O. , and J. , Four newly located pseudouridylate residues in Escherichia coli 23S ribosomal RNA are all at the peptidyltransferase center: Analysis by the application of a new sequencing technique, Biochemistry, vol.32, issue.37, pp.9754-9762, 1993.
DOI : 10.1021/bi00088a030

K. Nakai and M. Kanehisa, A knowledge base for predicting protein localization sites in eukaryotic cells, Genomics, vol.14, issue.4, pp.897-911, 1992.
DOI : 10.1016/S0888-7543(05)80111-9

A. Reinhardt and T. Hubbard, Using neural networks for prediction of the subcellular location of proteins, Nucleic Acids Research, vol.26, issue.9, pp.2230-2236, 1998.
DOI : 10.1093/nar/26.9.2230

J. Conrad, D. Sun, N. Englund, O. , and J. , Codes for a Pseudouridine Synthase That Is Solely Responsible for Synthesis of Pseudouridine at Positions 955, 2504, and 2580 in 23 S Ribosomal RNA, Journal of Biological Chemistry, vol.2, issue.29, pp.18562-18566, 1998.
DOI : 10.1073/pnas.95.7.3525

S. Raychaudhuri, J. Conrad, B. G. Hall, O. , and J. , A pseudouridine synthase required for the formation of two universally conserved pseudouridines in ribosomal RNA is essential for normal growth of Escherichia coli, RNA, vol.4, issue.11, pp.1407-1417, 1998.
DOI : 10.1017/S1355838298981146

J. Wrzesinski, A. Bakin, K. Nurse, B. G. Lane, O. et al., Purification, cloning, and properties of the 16S RNA pseudouridine 516 synthase from Escherichia coli, Biochemistry, vol.34, issue.27, pp.8904-8913, 1995.
DOI : 10.1021/bi00027a043

C. Bonnerot, L. Pintard, and G. Lutfalla, Functional Redundancy of Spb1p and a snR52-Dependent Mechanism for the 2???-O-Ribose Methylation of a Conserved rRNA Position in Yeast, Molecular Cell, vol.12, issue.5, pp.1309-1315, 2003.
DOI : 10.1016/S1097-2765(03)00435-0

J. Conrad, L. Niu, K. Rudd, B. G. Lane, O. et al., 16S ribosomal RNA pseudouridine synthase RsuA of Escherichia coli: Deletion, mutation of the conserved Asp102 residue, and sequence comparison among all other pseudouridine synthases, RNA, vol.5, issue.6, pp.751-763, 1999.
DOI : 10.1017/S1355838299990167

A. Oka, H. Sugisaki, and M. Takanami, Nucleotide sequence of the kanamycin resistance transposon Tn903, Journal of Molecular Biology, vol.147, issue.2, pp.217-226, 1981.
DOI : 10.1016/0022-2836(81)90438-1

P. F. Lue and J. G. Kaplan, The aspartate transcarbamylase and carbamoyl phosphate synthetase of yeast: A multi-functional enzyme complex, Biochemical and Biophysical Research Communications, vol.34, issue.4, pp.426-433, 1969.
DOI : 10.1016/0006-291X(69)90399-4

N. C. Martin and A. K. Hopper, How single genes provide tRNA processing enzymes to mitochondria, nuclei and the cytosol, Biochimie, vol.76, issue.12, pp.1161-1167, 1994.
DOI : 10.1016/0300-9084(94)90045-0

A. M. Rose, H. G. Belford, W. C. Shen, C. L. Greer, A. K. Hopper et al., Location of N2, N2-dimethylguanosine-specific tRNA methyltransferase, Biochimie, vol.77, issue.1-2, pp.45-53, 1995.
DOI : 10.1016/0300-9084(96)88103-X

D. Campo, M. Ofengand, J. Malhotra, and A. , Crystal structure of the catalytic domain of RluD, the only rRNA pseudouridine synthase required for normal growth of Escherichia coli, RNA, vol.10, issue.2, pp.231-239, 2004.
DOI : 10.1261/rna.5187404

M. Schaub and W. Keller, RNA editing by adenosine deaminases generates RNA and protein diversity, Biochimie, vol.84, issue.8, pp.791-803, 2002.
DOI : 10.1016/S0300-9084(02)01446-3