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QlAamp® DNA Stool Mini Kit -environ 200 mg de selles -1,4 ml de tampon ASl Vortexer jusqu'à homogénéisation complète Chauffer à 70°C pendant 5 min Vortexer 15 secondes Ajouter une tablette Inhibitex· Vortexer jusqu'à dissolution totale de la tablette Incuber à température ambiante pendant 1 min .:. Centrifuger à 20000 g pendant 3 min, Centrifuger à 20000 g pendant 1 min .:. Prélever 1,2 ml du surnageant dans un nouveau tube Eliminer le culot Pipeter tout le surnageant dans un nouveau tube .:. Centrifuger à 20000 g pendant 3 min .:. Mettre 15 pl, de Protéinase K dans un nouveau tube .:. Prélever 200 pl, du surnageant et le placer dans le tube contenant la Protéinase K .:. Ajouter 200 pl, de tampon Al Vortexer Incuber à 70°C pendant 10 min .:. Ajouter 200 pl, d'éthanol absolu au lysat et vortexer IV .:. Etiqueter les colonnes Qiagen Transférer tout le lysat sur la colonne Fermer le tube .:. Centrifuger à 20000 g pendant 1 min .:. Placer la colonne sur un nouveau tube collecteur et jeter l'ancien .:. Ajouter 500 IJL de tampon AW1 ,
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