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Master thesis

Etude prospective du MALDITOF sur la caractérisation fongique

Abstract : Phytopathogenic fungi present a harmful aspect economically and environmentally. It is thus essential to develop tools in order to characterize and identify in a fast and reliable way. Thanks to the advent of molecular biology in the last few decades, great progress has been made in the identifying process of these fungi, by combining a morphologic method with a “barcoding” one. In spite of this, it is currently still difficult to discriminate between taxonomic ranks inferior to the species one. Mass spectrometry, and especially Maldi-Tof, is a technique frequently used for bacterial and yeast identification, but less so for fungal organisms. The aim of this study is to determine if the Maldi-Tof can be routinely used to identify photopathogenic fungi via a universal protocol, then to examine up to which taxonomic rank this technology can discriminate. In order to do so, we will use the Fusarium genus, a complex genus including many pathogenic species.
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http://docnum.univ-lorraine.fr/prive/BUS_M_2015_THUILLIER_JORDHAN.pdf
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https://hal.univ-lorraine.fr/hal-01770446
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Submitted on : Thursday, April 19, 2018 - 9:10:58 AM
Last modification on : Thursday, April 19, 2018 - 9:10:59 AM

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  • HAL Id : hal-01770446, version 1

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Citation

Jordhan Thuillier. Etude prospective du MALDITOF sur la caractérisation fongique. Biotechnologies. 2015. ⟨hal-01770446⟩

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