Participation au projet LORVER et caractérisation de souches fongiques endophytes issues de sols pollués aux métaux

Abstract : The main aim of the LORVER phytoremediation project is to upgrade brownfields using poplar, hemp and nettle. The ability of these three plants to grow on metal-contaminated soils was evaluated and ICP-AES analyses revealed that heavy metals were localized in low amouts in the stems and the leaves. Fungal communities associated with the roots of the three species were observed by light microscopy. Arbuscular mycorrhizal fungi, but also fungal endophytes and dark septate endophytes (DSE) were identified. Fungal endophytes can affect heavy metal uptake of their host plants and increase plant metaltolerance. Therefore, in the present study, a wide-range screening of the fungal was performed to select efficient fungal symbionts. After studying the taxonomy of the isolated strains by molecular tools, their capacity to use diverse plant carbon compounds was evaluated. The positive effect of fungal inoculation on plant growth was revealed by a test with ryegrass and alfalfa. Finally, metal tolerance was variable between the studied strains, as well as their capacity to mobilize metals from the soil. In conclusion, Eu1-11, 2.2, CY1-2, 2.7 and Ce4, respectively belonging to the genera Cadophora, Leptodontidium and Phialocephala may be good candidates for future phytoremediation projects.
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Master thesis
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Charlotte Berthelot. Participation au projet LORVER et caractérisation de souches fongiques endophytes issues de sols pollués aux métaux. Sciences de l'environnement. 2013. ⟨hal-01779199⟩

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