, ces sphéroplastes se sont développés ce qui indique que le problème viendrait de la transformation et non pas de préparation des sphéroplastes. La souche qui devait servir d'hôte est une souche de S. coelicolor, la souche M1154, qui est a été spécialement créée pour être un hôte (Gomez-Escribano and Bibb, 2014) En effet les auteurs ont enlevé le les clusters de biosynthèse de l'actinorhodine, de la coelimycine, de la prodiginine et de l'antibiotique dépendant au calcium. De plus ils ont introduit des mutations dans les gènes rpoB (code pour la sous-unité ? de l'ARN polymérase) et rpsL (code une sous-unité de l'ARNr 30S, vecteur puis inoculés sur une boîte sans 5-FOA des mutations connues pour entraîner une augmentation de la production des différents antibiotiques sans impacter la croissance chez les Streptomyces, 1996.
, , 2002.
, ensemble des précautions à prendre pour réussir l'étape de transformation, l'ensemble des autres ilots seront alors clonés de la même manière dans le vecteur pCAP03. Les différents plasmides seront extraits puis transformés dans E. coli pour enfin être introduits dans S
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