, 0#3#4'0!:,%&!81!671235-87$8%241A)&!0,0!)77%131)&!&,0+!)0!B-301

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D. J. Adams, Fungal Cell Wall Chitinases and Glucanases ». Microbiology (Reading, 2004.

S. F. Altschul, T. Madden, . Schaffer, . Zhang, . Zhang et al., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Research, vol.25, issue.17, pp.3389-3402, 1997.
DOI : 10.1093/nar/25.17.3389

S. Antony-babu, . Deveau, . Van-nostrand, . Zhou, . Le-tacon et al., « Black Truffle-Associated Bacterial Communities during the Development and Maturation of Tuber Melanosporum Ascocarps and Putative Functional Roles, Environmental Microbiology

E. Barbieri, . Ceccaroli, C. Saltarelli, . Guidi, . Potenza et al., New evidence for nitrogen fixation within the Italian white truffle Tuber magnatum, Fungal Biology, vol.114, issue.11-12, 2010.
DOI : 10.1016/j.funbio.2010.09.001

E. Barbieri, . Guidi, P. Bertaux, J. Frey-klett, . Garbaye et al., Occurrence and Diversity of Bacterial Communities in Tuber Magnatum during Truffle Maturation, 2007.

F. Bellesia, . Pinetti, . Tirillini, and . Bianchi, « Temperature-Dependent Evolution of Volatile Organic Compounds in Tuber Borchii from Italy ». Flavour and Fragrance Journal 16, 1<1::AID-FFJ936>3.0.CO, pp.10-1002, 2001.

H. Bürgmann, V. Widmer, . Sigler, and . Zeyer, New Molecular Screening Tools for Analysis of Free-Living Diazotrophs in Soil, Applied and Environmental Microbiology, vol.70, issue.1, pp.240-247, 2004.
DOI : 10.1128/AEM.70.1.240-247.2004

C. Calvaruso, M. Turpault, E. Leclerc, and P. Frey-klett, Impact of Ectomycorrhizosphere on the Functional Diversity of Soil Bacterial and Fungal Communities from a Forest Stand in Relation to Nutrient Mobilization Processes, Microbial Ecology, vol.128, issue.3, pp.567-77, 2007.
DOI : 10.1590/S0001-37652006000100016

D. Cavazzini, . Meschi, . Corsini, G. Bolchi, O. Rossi et al., Journal of Biological Chemistry, vol.1522, issue.3, 2013.
DOI : 10.1074/jbc.M200264200

P. Ceccaroli, . Buffalini, . Saltarelli, . Barbieri, . Polidori et al., « Genomic Profiling of Carbohydrate Metabolism in the Ectomycorrhizal Fungus Tuber Melanosporum ». The New Phytologist 189, 2011.

G. Chevalier and . Frochot, Truffle cultivation techniques, 1997.

A. Coquelin, F. Torre, and R. Del-negro, « Modélisation de la formation de l'ascocarpe de la truffe noire Tuber melanosporum en fonction du couple humidité-température du sol, Ecologia mediterranea, pp.33-48, 2007.

P. Courty, K. Pritsch, . Schloter, . Hartmann, and . Garbaye, « Activity Profiling of Ectomycorrhiza Communities in Two Forest Soils Using Multiple Enzymatic Tests ». The New Phytologist 167, 2005.

D. Boer, J. H. Wietse, . Leveau, A. George, . Kowalchuk et al., Collimonas fungivorans gen. nov., sp. nov., a chitinolytic soil bacterium with the ability to grow on living fungal hyphae, INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol.54, issue.3, pp.857-64, 2004.
DOI : 10.1099/ijs.0.02920-0

N. Fierer and R. B. Jackson, The diversity and biogeography of soil bacterial communities, Proceedings of the National Academy of Sciences, vol.63, issue.12, pp.626-657, 2006.
DOI : 10.1128/AEM.70.9.5057-5065.2004

P. Frey-klett, M. Chavatte, . Clausse, . Courrier, . Le-roux et al., « Ectomycorrhizal Symbiosis Affects Functional Diversity of Rhizosphere Fluorescent Pseudomonads ». The New Phytologist 165, 2005.

P. Frey-klett, . Garbaye, and . Tarkka, « The Mycorrhiza Helper Bacteria Revisited ». The New Phytologist 176, 2007.

J. Gaby and . Buckley, A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase, PLoS ONE, vol.7, issue.7, 2012.
DOI : 10.1371/journal.pone.0042149.s019

P. Frey-klett, P. Burlinson, A. Deveau, M. Barret, M. Tarkka et al., Bacterial-Fungal Interactions: Hyphens between Agricultural, Clinical, Environmental, and Food Microbiologists, Microbiology and Molecular Biology Reviews, vol.75, issue.4, pp.583-609, 2011.
DOI : 10.1128/MMBR.00020-11

S. Ghio, G. Lorenzo, . Lia, . Talia, . Cataldi et al., « Isolation of Paenibacillus Sp. and Variovorax Sp. Strains from Decaying Woods and Characterization of Their Potential for Cellulose Deconstruction, International Journal of Biochemistry and Molecular Biology, vol.3, issue.4, pp.352-364, 2012.

I. Goldberg, . Nadler, and . Hochman, Mechanism of nitrogenase switch-off by oxygen., Journal of Bacteriology, vol.169, issue.2, pp.874-879, 1987.
DOI : 10.1128/jb.169.2.874-879.1987

M. Gryndler, . Soukupová, . Hr?elová, . Gryndlerová, . Borovi?ka et al., A quest for indigenous truffle helper prokaryotes, Environmental Microbiology Reports, vol.297, issue.3, 2013.
DOI : 10.1111/j.1574-6968.2009.01655.x

S. Hacquard, . Tisserant, . Brun, . Legué, . Martin et al., « Laser Microdissection and Microarray Analysis of Tuber Melanosporum Ectomycorrhizas Reveal Functional Heterogeneity between Mantle and Hartig Net Compartments, Environmental Microbiology, vol.15, issue.6, 2013.

I. R. Hall, G. Brown, and . Zambonelli, Taming the Truffle: The History, Lore, and Science of the Ultimate Mushroom, 2007.

E. Harki, D. Bouya, and E. R. Dargent, Maturation-associated alterations of the biochemical characteristics of the black truffle Tuber melanosporum Vitt. » Food Chemistry 99, 2006.

, JGI Fungi Portal -Home, 2014.

A. Kielak, . Cretoiu, . Semenov, . Sj-sørensen, and . Van-elsas, ABSTRACT, 263?272. doi:10.1128/AEM, pp.2546-2558, 2013.
DOI : 10.1128/AEM.02546-12

M. Koranda, . Kaiser, . Fuchslueger, . Kitzler, S. Sessitsch et al., Seasonal variation in functional properties of microbial communities in beech forest soil, Soil Biology and Biochemistry, vol.60, issue.100, 2013.
DOI : 10.1016/j.soilbio.2013.01.025

E. E. Kuramae, E. Yergeau, L. C. Wong, A. S. Pijl, J. A. Van-veen et al., Soil characteristics more strongly influence soil bacterial communities than land-use type, FEMS Microbiology Ecology, vol.326, issue.1, pp.12-24, 2012.
DOI : 10.1007/s11104-009-9988-y

C. L. Lauber, M. Hamady, R. Knight, and N. Fierer, Pyrosequencing-Based Assessment of Soil pH as a Predictor of Soil Bacterial Community Structure at the Continental Scale, Applied and Environmental Microbiology, vol.75, issue.15, pp.5111-5131, 2009.
DOI : 10.1128/AEM.00335-09

, Le Monde Des Truffes, 2014.

, Le Site Du Syndicat Des Trufficulteurs de La Région de Lalbenque, 2014.

L. Tacon, B. François, M. Marçais, C. Courvoisier, P. Murat et al., Climatic variations explain annual fluctuations in French P??rigord black truffle wholesale markets but do not explain the decrease in black truffle production over the last 48??years, Mycorrhiza, vol.180, issue.1, pp.115-125, 2014.
DOI : 10.1002/0471704091

C. Y. Li and M. Castellano, Azospirillum isolated from within sporocarps of the mycorrhizal fungi Hebeloma crustuliniforme, Laccaria laccata and Rhizopogon vinicolor, Transactions of the British Mycological Society, vol.88, issue.4, pp.563-565, 1987.
DOI : 10.1016/S0007-1536(87)80043-8

A. Ma, . Zhuang, . Wu, . Cui, C. Lv et al., Ascomycota Members Dominate Fungal Communities during Straw Residue Decomposition in Arable Soil, PLoS ONE, vol.30, issue.6, 2013.
DOI : 10.1371/journal.pone.0066146.s001

M. Mamoun, J. M. Et, and . Olivier, Dynamique des populations fongiques et bactériennes de la rhizosphère des noisetiers truffiers. II. Chélation du fer et répartition taxonomique chez les Pseudomonas fluorescents ». Agronomie 9, 1989.

F. Martin, C. Kohler, . Murat, . Balestrini, . Coutinho et al., P??rigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis, Nature, vol.3, issue.7291, p.7291, 2010.
DOI : 10.1038/nature08867

A. Mello, G. Ding, Y. Piceno, C. Napoli, L. Tom et al., Truffle Brûlés Have an Impact on the Diversity of Soil Bacterial Communities, 2013.

A. Mello, L. Miozzi, A. Vizzini, C. Napoli, G. Kowalchuck et al., ???productive niches, Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, vol.53, issue.2, pp.323-3210, 1080.
DOI : 10.1128/AEM.70.3.1723-1734.2004

A. Mello, . Murat, and . Bonfante, Truffles: much more than a prized and local fungal delicacy, FEMS Microbiology Letters, vol.260, issue.1, 2006.
DOI : 10.1111/j.1574-6968.2006.00252.x

URL : https://academic.oup.com/femsle/article-pdf/260/1/1/19120980/260-1-1.pdf

R. Nandakumar, S. Babu, T. Raguchander, and R. Samiyappan, Chitinolytic Activity of Native Pseudomonas Fluorescens Strains, pp.61-68, 2007.

J. Olivier, J. Savignac, and P. Sourzat, Truffe et trufficulture

G. Pacioni, D. Leonardi, . Carlo, . Ranalli, D. Zinni et al., Instrumental monitoring of the birth and development of truffles in a Tuber melanosporum orchard, Mycorrhiza, vol.114, issue.suppl, pp.65-72, 2014.
DOI : 10.1016/j.phytochem.2012.11.019

A. Pavi?, . Stankovi?, . Saljnikov, . Krüger, . Buscot et al., Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi, Fungal Ecology, vol.6, issue.6, 2013.
DOI : 10.1016/j.funeco.2013.05.006

H. Peter, . Beier, . Bertilsson, . Lindström, and . Langenheder, Function-specific response to depletion of microbial diversity, The ISME Journal, vol.9, issue.2, 2011.
DOI : 10.1111/j.1462-2920.2007.01335.x

URL : http://www.nature.com/ismej/journal/v5/n2/pdf/ismej2010119a.pdf

F. Poly, . Ranjard, . Nazaret, L. Gourbière, and . Monrozier, Comparison of nifH Gene Pools in Soils and Soil Microenvironments with Contrasting Properties, Applied and Environmental Microbiology, vol.67, issue.5, pp.2255-62, 2001.
DOI : 10.1128/AEM.67.5.2255-2262.2001

URL : http://aem.asm.org/content/67/5/2255.full.pdf

A. Pospiech and B. Neumann, A versatile quick-prep of genomic DNA from Gram-positive bacteria, Trends in Genetics, vol.11, issue.6, pp.217-235, 1995.
DOI : 10.1016/S0168-9525(00)89052-6

L. Riousset, . Riousset, M. Chevalier, and . Bardet, Truffes d'Europe et de Chine, 2001.

J. Rousk, E. Bååth, P. C. Brookes, C. L. Lauber, C. Lozupone et al., Soil bacterial and fungal communities across a pH gradient in an arable soil, The ISME Journal, vol.61, issue.10, pp.1340-51, 2010.
DOI : 10.1016/0168-1605(91)90063-U

URL : http://www.nature.com/ismej/journal/v4/n10/pdf/ismej201058a.pdf

. Rousset-rouard, L'avenir de la truffe face au réchauffement climatique: la truffe européenne est-elle en danger. Actes des 2es rencontres internationales de la truffe, 2008.

C. Sbrana, . Bagnoli, C. Bedini, . Filippi, M. Giovannetti et al., ascocarps, Canadian Journal of Microbiology, vol.95, issue.3, pp.259-68, 2000.
DOI : 10.1099/00221287-95-2-313

C. Sbrana, . Agnolucci, . Bedini, . Lepera, . Toffanin et al., mycelial growth, Diversity of Culturable Bacterial Populations Associated to Tuber Borchii Ectomycorrhizas and Their Activity on T. Borchii Mycelial Growth, pp.195-201, 2002.
DOI : 10.1093/treephys/16.11-12.1031

. Splivallo, A. Deveau, N. Valdez, N. Kirchhoff, P. Frey-klett et al., Bacteria associated with truffle-fruiting bodies contribute to truffle aroma, Environmental Microbiology, vol.56, issue.Suppl. 1, 2014.
DOI : 10.1007/s00294-009-0284-4

URL : https://hal.archives-ouvertes.fr/hal-01579586

D. Suryanto, N. Irawati, and E. Munir, Isolation and Characterization of Chitinolytic Bacteria and Their Potential to Inhibit Plant Pathogenic Fungi, Microbiology Indonesia, vol.5, issue.2, 2012.
DOI : 10.5454/mi.5.3.8

M. T. Tarkka and P. Frey-klett, Mycorrhiza Helper Bacteria, Mycorrhiza, edited by Professor Dr Ajit Varma, pp.113-145
DOI : 10.1007/978-3-540-78826-3_6

, Truffes L&Co Accessed, 2014.

S. Uroz, C. Calvaruso, M. Turpault, and P. Frey-klett, Mineral weathering by bacteria: ecology, actors and mechanisms, Trends in Microbiology, vol.17, issue.8, pp.378-87, 2009.
DOI : 10.1016/j.tim.2009.05.004

S. Uroz, C. Calvaruso, M. P. Turpault, J. C. Pierrat, C. Mustin et al., Effect of the Mycorrhizosphere on the Genotypic and Metabolic Diversity of the Bacterial Communities Involved in Mineral Weathering in a Forest Soil, Applied and Environmental Microbiology, vol.73, issue.9, pp.3019-271000121, 1128.
DOI : 10.1128/AEM.00121-07

URL : https://hal.archives-ouvertes.fr/hal-01195006

S. Uroz, P. Courty, J. Pierrat, . Peter, M. Buée et al., Functional Profiling and Distribution of the Forest Soil Bacterial Communities Along the Soil Mycorrhizosphere Continuum, Microbial Ecology, vol.4, issue.2, pp.404-419, 2013.
DOI : 10.1016/j.funeco.2011.04.002

URL : https://hal.archives-ouvertes.fr/hal-01001500

I. Vaz-moreira, C. Egas, O. Nunes, and C. Manaia, Culture-dependent and culture-independent diversity surveys target different bacteria: a case study in a freshwater sample, Antonie van Leeuwenhoek, vol.28, issue.12, 2009.
DOI : 10.3354/ame028141

J. Warmink, J. Aaldrik, and . Dirk-van-elsas, Selection of bacterial populations in the mycosphere of Laccaria proxima: is type III secretion involved?, The ISME Journal, vol.5, issue.8, pp.887-900, 2008.
DOI : 10.1038/ismej.2008.41

F. Widmer, L. Shaffer, . Porteous, and . Seidler, Analysis of nifH Gene Pool Complexity in Soil and Litter at a Douglas Fir Forest Site in the Oregon Cascade Mountain Range, Applied and Environmental Microbiology, vol.65, issue.2, 1999.

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