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, ? Milieu complet Milieu de base 500 mL Solution Glucose-Calcium 5 mL Le milieu complet est coulé dans des boîtes de Pétri
, Laisser se solidifier et conserver dans l'obscurité à 4 ± 2°C pendant 6 mois maximum s'il est bien protégé contre la dessiccation
, ssTYGA: Gélose semi-solide tryptone-extrait de levure-glucose Préparer un milieu de base conformément au TYGA, en utilisant seulement la moitié de la masse d'agar (8 g)
, Stockage : 4 ± 2°C à l'obscurité (6 mois maximum)
, RESUME
, environnement, la détermination de l'infectiosité des particules est indispensable à l'évaluation du risque sanitaire Ce travail vise à apporter des données sur l'intérêt des propriétés de surface (charge et balance hydrophile/hydrophobe) des particules virales pour la distinction entre virus infectieux et non infectieux. Les méthodes mises en oeuvre nécessitent l'obtention de virus purifiés
Elle aboutit à un niveau de pureté qui semble proche de la dialyse mais nécessite une optimisation plus importante. Dans un deuxième temps, nous avons utilisé la chromatographie à échange d'ions, l'étude de l'agrégation en fonction du pH et l'adhésion sur support hydrophobe pour comparer des phages MS2 infectieux et non infectieux ,