E. Marshall, Gene therapy death prompts review of adenovirus vector, Science, vol.286, pp.2244-2249, 1999.

S. Hacein-bey-abina, C. Von-kalle, M. Schmidt, L. Deist, F. Wulffraat et al., A serious adverse event after successful gene therapy for X-linked severe combined immunodeficiency, New Engl J Med, vol.348, pp.255-261, 2003.

C. Baum, J. Dullmann, Z. X. Li, B. Fehse, J. Meyer et al., Side effects of retroviral gene transfer into hematopoietic stem cells, Blood, vol.101, pp.2099-114, 2003.

H. Sakurai, K. Kawabata, F. Sakurai, S. Nakagawa, and H. Mizuguchi, Innate immune response induced by gene delivery vectors, Int J Pharm, vol.354, pp.9-15, 2008.

M. A. Mintzer and E. E. Simanek, Nonviral vectors for gene delivery, Chem Rev, vol.109, pp.259-302, 2009.

C. Q. Ding, A. W. Zhu, and Y. Tian, Functional surface engineering of C-Dots for fluorescent biosensing and in vivo bioimaging, Acc Chem Res, vol.47, pp.20-30, 2014.

C. J. Liu, P. Zhang, F. Tian, W. C. Li, F. Li et al., One-step synthesis of surface passivated carbon nanodots by microwave assisted pyrolysis for enhanced multicolor photoluminescence and bioimaging, J Mater Chem, vol.21, pp.13163-13170, 2011.

D. Gioia, S. Rejman, J. Carrabino, S. , D. Fino et al., Role of biophysical parameters on ex vivo and in vivo gene transfer to the airway epithelium by polyethylenimine/albumin complexes, Biomacromolecules, vol.9, pp.859-66, 2008.

P. Neuberg and A. Kichler, Non-viral vectors for gene therapy: Lipid-and polymer-based gene transfer, pp.263-288, 2014.

C. Liu, P. Zhang, X. Zhai, F. Tian, W. Li et al., Nano-carrier for gene delivery and bioimaging based on carbon dots with PEI-passivation enhanced fluorescence, Biomaterials, vol.33, pp.3604-3617, 2012.

J. Kim, J. Park, H. Kim, K. Singha, and W. J. Kim, Transfection and intracellular trafficking properties of carbon dot-gold nanoparticle molecular assembly conjugated with PEIpDNA, Biomaterials, vol.34, pp.7168-80, 2013.

L. M. Hu, Y. Sun, S. L. Li, X. L. Wang, K. L. Hu et al., Multifunctional carbon dots with high quantum yield for imaging and gene delivery, Carbon, vol.67, pp.508-521, 2014.

L. Wang, X. Wang, A. Bhirde, J. Cao, Y. Zeng et al., Carbon-dots-based twophoton visible nanocarriers for safe and highly efficient delivery of siRNA and DNA, Adv Healthcare Mater, vol.3, pp.1203-1212, 2014.

E. R. Lee, J. Marshall, C. S. Siegel, C. W. Jiang, N. S. Yew et al., Detailed analysis of structures and formulations of cationic lipids for efficient gene transfer to the lung, Hum Gene Ther, vol.7, pp.1701-1718, 1996.

U. Griesenbach, C. C. Vicente, M. J. Roberts, C. X. Meng, S. Soussi et al., Secreted Gaussia luciferase as a sensitive reporter gene for in vivo and ex vivo studies of airway gene transfer, Biomaterials, vol.32, pp.2614-2638, 2011.

M. A. Zanta, O. Boussif, A. Adib, and J. P. Behr, In vitro gene delivery to hepatocytes with galactosylated polyethylenimine, Bioconjugate Chem, vol.8, pp.839-883, 1997.
DOI : 10.1021/bc970098f

B. A. Tannous, Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo, Nat Protoc, vol.4, pp.582-91, 2009.
DOI : 10.1038/nprot.2009.28

URL : http://europepmc.org/articles/pmc2692611?pdf=render

Z. G. Dikmen, G. C. Gellert, S. Jackson, S. Gryaznov, R. Tressler et al., In vivo inhibition of lung cancer by GRN163L: a novel human telomerase inhibitor, Cancer Res, vol.65, pp.7866-73, 2005.

S. Foillard, G. Zuber, and E. Doris, Polyethylenimine-carbon nanotube nanohybrids for siRNA-mediated gene silencing at cellular level, Nanoscale, vol.3, pp.1461-1465, 2011.
DOI : 10.1039/c0nr01005g

O. A. Kucherak, S. Oncul, Z. Darwich, D. A. Yushchenko, Y. Arntz et al.,

, Switchable Nile Red-based probe for cholesterol and lipid order at the outer leaflet of biomembranes, J Am Chem Soc, vol.132, pp.4907-4923, 2010.

C. Ronzani, C. Spiegelhalter, J. L. Vonesch, L. Lebeau, and F. Pons, Lung deposition and toxicological responses evoked by multi-walled carbon nanotubes dispersed in a synthetic lung surfactant in the mouse, Arch Toxicol, vol.86, pp.137-186, 2012.

P. Pierrat, D. Kereselidze, P. Wehrung, G. Zuber, F. Pons et al., Bioresponsive deciduous-charge amphiphiles for liposomal delivery of DNA and siRNA, Pharm Res, vol.30, pp.1362-79, 2013.
DOI : 10.1007/s11095-013-0976-9

URL : https://hal.archives-ouvertes.fr/hal-02000403

S. N. Baker and G. A. Baker, Luminescent carbon nanodots: Emergent nanolights, Angew Chem-Int Ed, vol.49, pp.6726-6770, 2010.
DOI : 10.1002/anie.200906623

H. Zhu, X. L. Wang, Y. L. Li, Z. J. Wang, F. Yang et al., Microwave synthesis of fluorescent carbon nanoparticles with electrochemiluminescence properties, Chem Commun, pp.5118-5138, 2009.
DOI : 10.1039/b907612c

S. Chandra, P. Das, S. Bag, D. Laha, and P. Pramanik, Synthesis, functionalization and bioimaging applications of highly fluorescent carbon nanoparticles, Nanoscale, vol.3, pp.1533-1573, 2011.
DOI : 10.1039/c0nr00735h

Q. L. Wang, H. Z. Zheng, Y. J. Long, L. Y. Zhang, M. Gao et al., Microwave-hydrothermal synthesis of fluorescent carbon dots from graphite oxide, Carbon, vol.49, pp.3134-3174, 2011.
DOI : 10.1016/j.carbon.2011.03.041

X. Y. Zhai, P. Zhang, C. J. Liu, T. Bai, W. C. Li et al., Highly luminescent carbon nanodots by microwave-assisted pyrolysis, Chem Commun, vol.48, pp.7955-7962, 2012.
DOI : 10.1039/c2cc33869f

A. Jaiswal, S. S. Ghosh, and A. Chattopadhyay, One step synthesis of C-dots by microwave mediated caramelization of poly(ethylene glycol), Chem Commun, vol.48, pp.407-416, 2012.

N. Puvvada, B. Kumar, S. Konar, H. Kalita, M. Mandal et al., Synthesis of biocompatible multicolor luminescent carbon dots for bioimaging applications, Sci Technol Adv Mater, vol.13, 2012.

X. Y. Qin, W. B. Lu, A. M. Asiri, A. O. Al-youbi, and X. P. Sun, Microwave-assisted rapid green synthesis of photoluminescent carbon nanodots from flour and their applications for sensitive and selective detection of mercury(II) ions, Sensor Actuat B-Chem, vol.184, pp.156-62, 2013.

S. Dey, P. Chithaiah, S. Belawadi, K. Biswas, and C. Rao, New methods of synthesis and varied properties of carbon quantum dots with high nitrogen content, J Mater Res, vol.29, pp.383-91, 2014.

Q. Hu, M. C. Paau, Y. Zhang, X. J. Gong, L. Zhang et al., Green synthesis of fluorescent nitrogen/sulfurdoped carbon dots and investigation of their properties by HPLC coupled with mass spectrometry, RSC Adv, vol.4, pp.18065-73, 2014.

Y. Liu, N. Xiao, N. Q. Gong, H. Wang, X. Shi et al., One-step microwave-assisted polyol synthesis of green luminescent carbon dots as optical nanoprobes, Carbon, vol.68, pp.258-64, 2014.

W. Wang, Y. M. Li, L. Cheng, Z. Q. Cao, and W. G. Liu, Water-soluble and phosphoruscontaining carbon dots with strong green fluorescence for cell labeling, J Mat Chem B, vol.2, pp.46-54, 2014.

Q. Zhao, Z. Zhang, B. Huang, J. Peng, M. Zhang et al., Facile preparation of low cytotoxicity fluorescent carbon nanocrystals by electrooxidation of graphite, Chem Commun, pp.5116-5124, 2008.

S. L. Hu, J. Liu, J. L. Yang, Y. Z. Wang, and S. R. Cao, Laser synthesis and size tailor of carbon quantum dots, J Nanopart Res, vol.13, pp.7247-52, 2011.

A. Sachdev, I. Matai, and P. Gopinath, Implications of surface passivation on physicochemical and bioimaging properties of carbon dots, RSC Adv, vol.4, pp.20915-20936, 2014.

M. Y. Liu, X. Q. Zhang, B. Yang, F. J. Deng, J. H. Ji et al., Luminescence tunable fluorescent organic nanoparticles from polyethyleneimine and maltose: facile preparation and bioimaging applications, RSC Adv, vol.4, pp.22294-22302, 2014.

M. K. Geiger-j-aneja and C. Rudolph, Vectors for pulmonary gene therapy, Int J Pharm, vol.390, pp.84-92, 2010.

M. Beck-broichsitter, O. M. Merkel, and T. Kissel, Controlled pulmonary drug and gene delivery using polymeric nano-carriers, J Control Release, vol.161, pp.214-238, 2012.

S. H. Van-rijt, T. Bein, and S. Meiners, Medical nanoparticles for next generation drug delivery to the lungs, Eur Respir J, vol.44, pp.365-74, 2014.

C. L. Densmore, F. M. Orson, B. Xu, B. M. Kinsey, J. C. Waldrep et al., Aerosol delivery of robust polyethyleneimine-DNA complexes for gene therapy and genetic immunization, Mol Ther, vol.1, pp.180-188, 2000.

L. A. Davies, G. Mclachlan, S. G. Sumner-jones, D. Ferguson, A. Baker et al., Enhanced lung gene expression after aerosol delivery of concentrated pDNA/PEI complexes, Mol Ther, vol.16, pp.1283-90, 2008.

A. E. Stern, M. Farley, R. Jaffe, A. Chadwick, S. L. Phillips et al., Cationic lipidmediated CFTR gene transfer to the lungs and nose of patients with cystic fibrosis: a double-blind placebo-controlled trial, Lancet, vol.353, pp.947-54, 1999.

G. Mclachlan, H. Davidson, E. Holder, L. A. Davies, I. A. Pringle et al., Pre-clinical evaluation of three non-viral gene transfer agents for cystic fibrosis after aerosol delivery to the ovine lung, Gene Ther, vol.18, pp.996-1005, 2011.

A. E. Boyd, A. C. Cheng, S. H. Davies, J. C. Davies, L. A. Dayan et al., Toxicology study assessing efficacy and safety of repeated administration of lipid/DNA complexes to mouse lung, Gene Ther, vol.21, pp.89-95, 2014.

W. M. Saltzman, M. L. Radomsky, K. J. Whaley, and R. A. Cone, Antibody diffusion in human cervical-mucus, Biophys J, vol.66, pp.508-523, 1994.

S. S. Olmsted, J. L. Padgett, A. I. Yudin, K. J. Whaley, T. R. Moench et al., Diffusion of macromolecules and virus-like particles in human cervical mucus, Biophys J, vol.81, pp.1930-1937, 2001.

S. K. Lai, D. E. O'hanlon, H. S. Man, S. T. Wang, Y. Y. Cone et al., Rapid transport of large polymeric nanoparticles in fresh undiluted human mucus, Proc Natl Acad Sci U S A, vol.104, pp.1482-1489, 2007.

B. S. Schuster, J. S. Suk, G. F. Woodworth, and J. Hanes, Nanoparticle diffusion in respiratory mucus from humans without lung disease, Biomaterials, vol.34, pp.3439-3485, 2013.

Y. Ikeda and Y. Nagasaki, Impacts of PEGylation on the gene and oligonucleotide delivery system, J Appl Polym Sci, vol.131, p.10, 2014.

U. Wattendorf and H. P. Merkle, PEGylation as a tool for the biomedical engineering of surface modified microparticles, J Pharm Sci, vol.97, pp.4655-69, 2008.

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