Interpretation of Real-Time PCR Results for Hepatitis C Virus RNA When Viral Load Is Below Quantification Limits

Syria Laperche 1 Françoise Bouchardeau 2 Vincent Thibault 3 Anne-Marie Roque-Afonso 4 Pascale Trimoulet 5 Ronald Colimon 3 Gilles Duverlie 6 Hélène Leguillou-Guillemette Françoise Lunel 7 Magali Bouvier-Alias 1 Michel Pawlotsky Cécile Henquell 8 Evelyne Schvoerer 9 Françoise Stoll-Keller 10 Marie-Laure Chaix 11 Michel Branger 12 Catherine Gaudy-Graffin 13 Arielle Rosenberg 14, 15 Bruno Pozzetto 16 Sophie Vallet 17 Yazid Baazia 18 Jacques Izopet 19 Jean-Jacques Lefrere 20, 21 Elisabeth André-Garnier 22
Abstract : Hepatitis C virus RNA quantification results obtained in 18 laboratories using real-time PCR methods with 10 negative samples and 22 sample dilutions (viral loads of 0.5 to 500 IU/ml) showed a score of correct results of up to 93.5%. However, 55.6% of the laboratories did not follow the recommendations for the interpretation of their results, leading to ambiguous conclusions.
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https://hal.univ-lorraine.fr/hal-02014377
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Submitted on : Monday, February 11, 2019 - 3:38:50 PM
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Syria Laperche, Françoise Bouchardeau, Vincent Thibault, Anne-Marie Roque-Afonso, Pascale Trimoulet, et al.. Interpretation of Real-Time PCR Results for Hepatitis C Virus RNA When Viral Load Is Below Quantification Limits. Journal of Clinical Microbiology, American Society for Microbiology, 2011, 49 (3), pp.1113-1115. ⟨10.1128/JCM.01511-10⟩. ⟨hal-02014377⟩

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