Interpretation of Real-Time PCR Results for Hepatitis C Virus RNA When Viral Load Is Below Quantification Limits - Université de Lorraine Access content directly
Journal Articles Journal of Clinical Microbiology Year : 2011

Interpretation of Real-Time PCR Results for Hepatitis C Virus RNA When Viral Load Is Below Quantification Limits

Anne-Marie Roque-Afonso
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Gilles Duverlie
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Hélène Leguillou-Guillemette
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Michel Pawlotsky
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Michel Branger
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Jacques Izopet

Abstract

Hepatitis C virus RNA quantification results obtained in 18 laboratories using real-time PCR methods with 10 negative samples and 22 sample dilutions (viral loads of 0.5 to 500 IU/ml) showed a score of correct results of up to 93.5%. However, 55.6% of the laboratories did not follow the recommendations for the interpretation of their results, leading to ambiguous conclusions.

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hal-02014377 , version 1 (11-02-2019)

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Syria Laperche, Françoise Bouchardeau, Vincent Thibault, Anne-Marie Roque-Afonso, Pascale Trimoulet, et al.. Interpretation of Real-Time PCR Results for Hepatitis C Virus RNA When Viral Load Is Below Quantification Limits. Journal of Clinical Microbiology, 2011, 49 (3), pp.1113-1115. ⟨10.1128/JCM.01511-10⟩. ⟨hal-02014377⟩
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