Caractérisation immunologique de plusieurs protéines végétales impliquées dans l'allergie alimentaire en vue de la création d'un test de diagnostic sur biopuces

Abstract : Food allergy triggers many restrictions in allergic patients' life. A new and more accurate diagnostic test has to be elaborated since the currently available tests are not accurate enough to determine precisely the allergic profile of a patient. A couple of research teams decided to share their knowledge to elaborate a macroarray as a diagnostic tool. This tool enables to test 120 different foods in one trial. In 6 months, 17 extracts of different cereals, spices, legumes and nuts have been produced and characterized by SDS-PAGE.. Six sera from peanut allergic patient have been characterized with immunobloting (western-blot). Moreover some experimental conditions concerning the macroarray tests have been determined thanks to a "biopuce" designed with peanut extract and 3 purified peanut allergens. The tests were carried out with the previous characterized sera and the best conditions were: dilution of the serum 1/5 and its incubation time 1h30. However a lot of new data have to be acquired before being able to statistically assert the validity of this new "biopuce" as a diagnostic tool.
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Master thesis
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Manon Morin. Caractérisation immunologique de plusieurs protéines végétales impliquées dans l'allergie alimentaire en vue de la création d'un test de diagnostic sur biopuces. Sciences de l'environnement. 2012. ⟨hal-02093605⟩

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