Antagonisme entre les espèces du complexe Scedosporium apiospermum et Pseudomonas aeruginosa chez les patients atteints de mucoviscidose : caractérisation des molécules inhibitrices

Abstract : In the context of cystic fibrosis, Staphylococcus aureus and Pseudomonas aeruginosa are responsible for many infection of respiratory tract. Eighty percent patients are colonized/infected by P. aeruginosa. One the other hand, the genus Scedosporium, is the second filamentous fungal agent colonizing and infecting the lung of cystic fibrosis patient'. Among patients with cystic fibrosis an antagonism have been described inter P.aeruginosa and the species complex Scedosporium, which Pseudallescheria boydii.This antagonism had been found previously in the GEIHP laboratory. The aim of this work has been to validate this antagonism and to identify the antimicrobial molecules. Antagonism between these two strains could not be confirmed, but it have been demonstrated in this work that the broth of P.boydii15155 (IHEM) has activity anti Staphylococcus aureus and epidermidis. An extraction has been realized in the culture broth, and the extracts cyclohexan, dichloromethane and acetate d'ethyl have been showed an antibacterial activity. The secreted metabolite(s) by P.boydii have been purified and characterized by HPLC method coupled to ground. The HPLC/MS/UV data ofcyclohexan to extract show similarities with the analytical data of the literature the dehydroxybisdethiobis(methyl thio)gliotoxin, active against Staphylococcus aureus. Analysis of other samples suggests the presence of chemical structures original from P. boydii.
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Master thesis
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Cindy Staerck. Antagonisme entre les espèces du complexe Scedosporium apiospermum et Pseudomonas aeruginosa chez les patients atteints de mucoviscidose : caractérisation des molécules inhibitrices. Microbiologie et Parasitologie. 2014. ⟨hal-02096587⟩

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