<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-02902639</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-02T19:14:05+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Optimising the diagnostic strategy for onychomycosis from sample collection to FUNGAL identification evaluation of a diagnostic kit for real-time PCR</title>
            <author role="aut">
              <persName>
                <forename type="first">Dimitri</forename>
                <surname>Petinataud</surname>
              </persName>
              <idno type="halauthorid">1148622-0</idno>
              <affiliation ref="#struct-177196"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Sibel</forename>
                <surname>Berger</surname>
              </persName>
              <email type="md5">32071a24b73dbd97bc7c07e0ebba3e47</email>
              <email type="domain">gmail.com</email>
              <idno type="idhal" notation="string">sibel-berger</idno>
              <idno type="idhal" notation="numeric">4150</idno>
              <idno type="halauthorid" notation="string">8974-4150</idno>
              <idno type="IDREF">https://www.idref.fr/083811559</idno>
              <affiliation ref="#struct-487302"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Cyril</forename>
                <surname>Ferdynus</surname>
              </persName>
              <idno type="halauthorid">471115-0</idno>
              <affiliation ref="#struct-351465"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Anne</forename>
                <surname>Debourgogne</surname>
              </persName>
              <email type="md5">1ea7046c603ca00dbb3b5859b25c4c9b</email>
              <email type="domain">univ-lorraine.fr</email>
              <idno type="idhal" notation="string">anne-debourgogne</idno>
              <idno type="idhal" notation="numeric">762432</idno>
              <idno type="halauthorid" notation="string">450275-762432</idno>
              <idno type="IDREF">https://www.idref.fr/135395410</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-1240-856X</idno>
              <affiliation ref="#struct-177196"/>
              <affiliation ref="#struct-425853"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Nelly</forename>
                <surname>Contet-Audonneau</surname>
              </persName>
              <idno type="halauthorid">717607-0</idno>
              <affiliation ref="#struct-177196"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Marie</forename>
                <surname>Machouart</surname>
              </persName>
              <email type="md5">6a1f2329c919fadafa3049df80bc3fce</email>
              <email type="domain">univ-lorraine.fr</email>
              <idno type="idhal" notation="numeric">1093248</idno>
              <idno type="halauthorid" notation="string">450277-1093248</idno>
              <idno type="IDREF">https://www.idref.fr/135397340</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-7510-6211</idno>
              <affiliation ref="#struct-177196"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Anne</forename>
                <surname>Debourgogne</surname>
              </persName>
              <email type="md5">1ea7046c603ca00dbb3b5859b25c4c9b</email>
              <email type="domain">univ-lorraine.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2020-07-20 10:37:09</date>
              <date type="whenModified">2025-02-20 15:35:37</date>
              <date type="whenReleased">2020-07-20 10:37:09</date>
              <date type="whenProduced">2016-05</date>
              <ref type="externalLink" target="https://api.istex.fr/ark:/67375/WNG-SL497QZ9-D/fulltext.pdf?sid=hal"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="906309">
                <persName>
                  <forename>Anne</forename>
                  <surname>Debourgogne</surname>
                </persName>
                <email type="md5">1ea7046c603ca00dbb3b5859b25c4c9b</email>
                <email type="domain">univ-lorraine.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-02902639</idno>
            <idno type="halUri">https://hal.univ-lorraine.fr/hal-02902639</idno>
            <idno type="halBibtex">petinataud:hal-02902639</idno>
            <idno type="halRefHtml">&lt;i&gt;Mycoses&lt;/i&gt;, 2016, 59 (5), pp.304-311. &lt;a target="_blank" href="https://dx.doi.org/10.1111/myc.12471"&gt;&amp;#x27E8;10.1111/myc.12471&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Mycoses, 2016, 59 (5), pp.304-311. &amp;#x27E8;10.1111/myc.12471&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="UNIV-LORRAINE">Université de Lorraine</idno>
            <idno type="stamp" n="SIMPA-UL">SIMPA - Laboratoire Stress, IMmunité, PAthogènes</idno>
            <idno type="stamp" n="BMS-UL">Pôle scientifique Biologie, Médecine, Santé de l'Université de Lorraine</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Optimising the diagnostic strategy for onychomycosis from sample collection to FUNGAL identification evaluation of a diagnostic kit for real-time PCR</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Dimitri</forename>
                    <surname>Petinataud</surname>
                  </persName>
                  <idno type="halauthorid">1148622-0</idno>
                  <affiliation ref="#struct-177196"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Sibel</forename>
                    <surname>Berger</surname>
                  </persName>
                  <email type="md5">32071a24b73dbd97bc7c07e0ebba3e47</email>
                  <email type="domain">gmail.com</email>
                  <idno type="idhal" notation="string">sibel-berger</idno>
                  <idno type="idhal" notation="numeric">4150</idno>
                  <idno type="halauthorid" notation="string">8974-4150</idno>
                  <idno type="IDREF">https://www.idref.fr/083811559</idno>
                  <affiliation ref="#struct-487302"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Cyril</forename>
                    <surname>Ferdynus</surname>
                  </persName>
                  <idno type="halauthorid">471115-0</idno>
                  <affiliation ref="#struct-351465"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Anne</forename>
                    <surname>Debourgogne</surname>
                  </persName>
                  <email type="md5">1ea7046c603ca00dbb3b5859b25c4c9b</email>
                  <email type="domain">univ-lorraine.fr</email>
                  <idno type="idhal" notation="string">anne-debourgogne</idno>
                  <idno type="idhal" notation="numeric">762432</idno>
                  <idno type="halauthorid" notation="string">450275-762432</idno>
                  <idno type="IDREF">https://www.idref.fr/135395410</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-1240-856X</idno>
                  <affiliation ref="#struct-177196"/>
                  <affiliation ref="#struct-425853"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Nelly</forename>
                    <surname>Contet-Audonneau</surname>
                  </persName>
                  <idno type="halauthorid">717607-0</idno>
                  <affiliation ref="#struct-177196"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Marie</forename>
                    <surname>Machouart</surname>
                  </persName>
                  <email type="md5">6a1f2329c919fadafa3049df80bc3fce</email>
                  <email type="domain">univ-lorraine.fr</email>
                  <idno type="idhal" notation="numeric">1093248</idno>
                  <idno type="halauthorid" notation="string">450277-1093248</idno>
                  <idno type="IDREF">https://www.idref.fr/135397340</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-7510-6211</idno>
                  <affiliation ref="#struct-177196"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">17251</idno>
                <idno type="issn">0933-7407</idno>
                <idno type="eissn">1439-0507</idno>
                <title level="j">Mycoses</title>
                <imprint>
                  <publisher>Wiley</publisher>
                  <biblScope unit="volume">59</biblScope>
                  <biblScope unit="issue">5</biblScope>
                  <biblScope unit="pp">304-311</biblScope>
                  <date type="datePub">2016-05</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1111/myc.12471</idno>
              <idno type="pubmed">26806228</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">Onychomycosis</term>
                <term xml:lang="en">PCR</term>
                <term xml:lang="en">Kit</term>
                <term xml:lang="en">Direct examination</term>
                <term xml:lang="en">Culture</term>
                <term xml:lang="en">Diagnosis</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv.mp.myc">Life Sciences [q-bio]/Microbiology and Parasitology/Mycology</classCode>
              <classCode scheme="halDomain" n="sdv.mp.par">Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Onychomycosis is a common nail disorder mainly due to dermatophytes for which the conventional diagnosis requires direct microscopic observation and culture of a biological sample. Nevertheless, antifungal treatments are commonly prescribed without a mycological examination having been performed, partly because of the slow growth of dermatophytes. Therefore, molecular biology has been applied to this pathology, to support a quick and accurate distinction between onychomycosis and other nail damage. Commercial kits are now available from several companies for improving traditional microbiological diagnosis. In this paper, we present the first evaluation of the real-time PCR kit marketed by Bio Evolution for the diagnosis of dermatophytosis. Secondly, we compare the efficacy of the kit on optimal and non-optimal samples. This study was conducted on 180 nails samples, processed by conventional methods and retrospectively analysed using this kit. According to our results, this molecular kit has shown high specificity and sensitivity in detecting dermatophytes, regardless of sample quality. On the other hand, and as expected, optimal samples allowed the identification of a higher number of dermatophytes by conventional mycological diagnosis, compared to non-optimal samples. Finally, we have suggested several strategies for the practical use of such a kit in a medical laboratory for quick pathogen detection.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-177196" status="INCOMING">
          <orgName>Service de Parasitologie-Mycologie [CHRU Nancy]</orgName>
          <desc>
            <address>
              <addrLine>11 Allée du Morvan;54511;Vandoeuvre-lès-Nancy</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-300157" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-487302" status="VALID">
          <orgName>Service de Virologie [CHRU Nancy]</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-300157" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-351465" status="VALID">
          <idno type="ROR">https://ror.org/004dan487</idno>
          <orgName>Centre Hospitalier Universitaire de La Réunion</orgName>
          <orgName type="acronym">CHU La Réunion</orgName>
          <date type="start">2012-02-01</date>
          <desc>
            <address>
              <country key="RE"/>
            </address>
            <ref type="url">https://www.chu-reunion.fr</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-425853" status="VALID">
          <idno type="IdRef">176429638</idno>
          <idno type="RNSR">201320858V</idno>
          <idno type="IdUnivLorraine">[UL]RND--</idno>
          <idno type="ROR">https://ror.org/04f50n919</idno>
          <orgName>Stress, Immunité, Pathogènes</orgName>
          <orgName type="acronym">SIMPA</orgName>
          <date type="start">2013-01-01</date>
          <desc>
            <address>
              <addrLine>Université de Lorraine, Faculté de Médecine, 9 avenue de la Forêt de Haye, 54500 Vandoeuvre-les-Nancy</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://simpa.univ-lorraine.fr/</ref>
          </desc>
          <listRelation>
            <relation name="UR7300" active="#struct-413289" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300157" status="VALID">
          <orgName>Centre Hospitalier Régional Universitaire de Nancy</orgName>
          <orgName type="acronym">CHRU Nancy</orgName>
          <desc>
            <address>
              <addrLine>Siège social : 29 avenue du Maréchal de Lattre de Tassigny, CO 60034, 54035 NANCY CEDEXHôpital Central : 29 Avenue du Maréchal de Lattre de Tassigny, 54035 NancyHôpitaux de Brabois / Enfants : Rue du Morvan, 54500 Vandœuvre-lès-NancyMaternité régionale :10 Avenue Dr Heydenreich, 54000 Nancy</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.chu-nancy.fr</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-413289" status="VALID">
          <idno type="IdRef">157040569</idno>
          <idno type="IdUnivLorraine">[UL]100--</idno>
          <idno type="ROR">https://ror.org/04vfs2w97</idno>
          <orgName>Université de Lorraine</orgName>
          <orgName type="acronym">UL</orgName>
          <date type="start">2012-01-01</date>
          <desc>
            <address>
              <addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-lorraine.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>