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Metabolomics approach based on LC-HRMS for the fast screening of iron(II)-chelating peptides in protein hydrolysates

Abstract : Production of iron-chelating peptides from protein hydrolysates requires robust and adequate screening methods to optimize theirpurification and subsequently valorize their potential antioxidant properties. An original methodology was developed for directand sensitive screening of iron(II)-chelating peptides based on ion-pair reverse phase liquid chromatography (IP-RPLC) coupledto high-resolution mass spectrometry (HRMS). Peptide mixture was first added to iron(II) solution to form iron(II)-peptidecomplexes. Then IP-RPLC-HRMS analysis was conducted on this iron-peptide mixture and on the iron-free peptide solutionfor comparative mass spectra analysis. This protocol, initially applied to a range of low molecular weight standard peptides,allowed detection of [(Peptide-H)+56FeII]+complex ion for iron(II)-chelating peptides (GGH, EAH, DAH,βAH, DMH, DTH,DSH). GGH was added in complex peptide mixtures and targeted analysis of [(GGH-H)+56FeII]+complex showed a limit ofdetection (LOD) below 0.77 mg L−1of GGH. This protocol was finally tested in combination with metabolomics software andadditional digital processing for non-targeted search for iron(II)-chelating peptides. Applicability of this new screening method-ology has been validated by detection of GGH as iron(II)-chelating peptide when added at 0.77 mg L−1in casein hydrolysate.
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https://hal.univ-lorraine.fr/hal-03175762
Contributor : Claire Gaiani <>
Submitted on : Sunday, March 21, 2021 - 10:37:40 AM
Last modification on : Tuesday, August 10, 2021 - 10:12:15 AM

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Cédric Paris, Katalin Selmeczi, Bruno Ebel, Loïc Stefan, Gizella Csire, et al.. Metabolomics approach based on LC-HRMS for the fast screening of iron(II)-chelating peptides in protein hydrolysates. Analytical and Bioanalytical Chemistry, Springer Verlag, 2021, 413 (2), pp.315-329. ⟨10.1007/s00216-020-03037-1⟩. ⟨hal-03175762⟩

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