Skip to Main content Skip to Navigation
Journal articles

Electrically Switchable Nanolever Technology for the Screening of Metal-Chelating Peptides in Hydrolysates

Abstract : Metal-chelating peptides (MCP) are considered as indirect antioxidants due to their capacity to inhibit radical chain reaction and oxidation. Here, we propose a new proof of concept for the screening of MCPs present in protein hydrolysates for valorizing their antioxidant properties by using the emerging time-resolved molecular dynamics technology, switchSENSE. This method unveils possible interactions between MCPs and immobilized nickel ions using fluorescence and electro-switchable DNA chips. The switchSENSE method was first set up on synthetic peptides known for their metal-chelating properties. Then, it was applied to soy and tilapia viscera protein hydrolysates. Their Cu2+-chelation capacity was, in addition, determined by UV-visible spectrophotometry as a reference method. The switchSENSE method has displayed a high sensitivity to evidence the presence of MCPs in both hydrolysates. Hence, we demonstrate for the first time that this newly introduced technology is a convenient methodology to screen protein hydrolysates in order to determine the presence of MCPs before launching time-consuming separations.
Document type :
Journal articles
Complete list of metadata

https://hal.univ-lorraine.fr/hal-03324543
Contributor : Thomas Chaigneau <>
Submitted on : Monday, August 23, 2021 - 4:48:01 PM
Last modification on : Saturday, September 11, 2021 - 3:32:22 AM

Identifiers

Citation

Sarah El Hajj, Cindy Tatiana Sepúlveda Rincón, Jean-Michel Girardet, Céline Cakir-Kiefer, Loic Stefan, et al.. Electrically Switchable Nanolever Technology for the Screening of Metal-Chelating Peptides in Hydrolysates. Journal of Agricultural and Food Chemistry, American Chemical Society, 2021, 69 (31), pp.8819-8827. ⟨10.1021/acs.jafc.1c02199⟩. ⟨hal-03324543⟩

Share

Metrics

Record views

21