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Article Dans Une Revue Journal of Agricultural and Food Chemistry Année : 2021

Electrically switchable nanolever technology for the screening of metal-chelating peptides in hydrolysates

Résumé

Metal-chelating peptides (MCP) are considered as indirect antioxidants due to their capacity to inhibit radical chain reaction and oxidation. Here, we propose a new proof of concept for the screening of MCPs present in protein hydrolysates for valorizing their antioxidant properties by using the emerging time-resolved molecular dynamics technology, switchSENSE. This method unveils possible interactions between MCPs and immobilized nickel ions using fluorescence and electro-switchable DNA chips. The switchSENSE method was first set up on synthetic peptides known for their metal-chelating properties. Then, it was applied to soy and tilapia viscera protein hydrolysates. Their Cu2+-chelation capacity was, in addition, determined by UV-visible spectrophotometry as a reference method. The switchSENSE method has displayed a high sensitivity to evidence the presence of MCPs in both hydrolysates. Hence, we demonstrate for the first time that this newly introduced technology is a convenient methodology to screen protein hydrolysates in order to determine the presence of MCPs before launching time-consuming separations.
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Dates et versions

hal-03447722 , version 1 (24-11-2021)

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Sarah El Hajj, Cindy Tatiana Sepúlveda Rincón, Jean-Michel Girardet, Céline Cakir-Kiefer, Loïc Stefan, et al.. Electrically switchable nanolever technology for the screening of metal-chelating peptides in hydrolysates. Journal of Agricultural and Food Chemistry, 2021, 69 (31), pp.8819-8827. ⟨10.1021/acs.jafc.1c02199⟩. ⟨hal-03447722⟩
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