Physical enrichment of structures has been used for the last decades in aquaculture to improve fish production and welfare. Until now, this enrichment has been practiced in fish monoculture but not in fish polyculture. In this study, we developed a polyculture of two freshwater species (pikeperch and sterlet) in recirculated systems (tank of 2.4 m(3)) with or without physical structures for enrichment. Two types of structures were used: a cover plank on a part of the tank decreasing the light intensity and vertical pipes modifying the water flow. The experiment was conducted in triplicate for a three-month period with juvenile fishes (143 +/- 41 g and 27.3 +/- 2.2 cm for pikeperch and 133 +/- 21 g and 32.8 +/- 1.6 cm for sterlet). Behavioural (space occupation and abnormal behaviours) and morphological (total length, final weight, Fulton condition factor, coefficient of variation of the final weight, percentage of biomass gain and specific growth rate) traits were measured. The pikeperch changed their space occupation and showed a preference for low light areas. Sterlet also changed their space occupation: they did not use the cover and occurred mainly in the part of the tank without enrichment. There was no difference for the frequency of abnormal behaviours for pikeperch and sterlet between the two sets (with or without enrichment). There was no statistical difference between the two sets for all the morphological and growth parameters no matter the species and the rearing modality.