Understanding the Role of Surface Interactions in the Antibacterial Activity of Layered Double Hydroxide Nanoparticles by Atomic Force Microscopy - Université de Lorraine Access content directly
Journal Articles Nanoscale Year : 2022

Understanding the Role of Surface Interactions in the Antibacterial Activity of Layered Double Hydroxide Nanoparticles by Atomic Force Microscopy

Abstract

Understanding the mechanisms of interactions between zinc-based layered double hydroxides (LDHs) and bacterial surfaces becomes of great importance to improve the efficiency of these antibiotic-free antibacterial agents. In fact, the role of surface interactions in the antibacterial activity of zinc-based LDH nanoparticles compared to that of dissolution and generation of reactive oxygen species (ROS) is still not well documented. In this study we show that synthesized ZnAl LDH nanoparticles exhibit a strong antibacterial effect against Staphylococcus aureus and cause serious cell wall damages as revealed by growth test and atomic force microscopy (AFM) imaging, respectively. The comparison of the antibacterial properties of ZnAl LDH nanoparticles and micron-sized ZnAl LDHs also allowed to determine that the antibacterial activity of Zn-based LDHs go beyond the simple dissolution into Zn2+ antibacterial ions. Furthermore, we developed an original approach to functionalize AFM tips with LDH films in order to probe their interactions with live S. aureus cells by means of AFM-based force spectroscopy (FS). The force spectroscopy analysis revealed that antibacterial ZnAl LDH nanoparticles possess a specific recognition to S. aureus cells with high adhesion frequency and remarkable force magnitudes. Such finding provides a first insight about the antibacterial mechanism of Zn-based LDHs by direct surface interactions through which they are able to recognize and adhere to bacterial surfaces which lead to their damage and subsequent growth inhibition.
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Dates and versions

hal-03705956 , version 1 (27-06-2022)

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Jazia Awassa, Samantha Soulé, Damien Cornu, Christian Ruby, Sofiane El-Kirat-Chatel. Understanding the Role of Surface Interactions in the Antibacterial Activity of Layered Double Hydroxide Nanoparticles by Atomic Force Microscopy. Nanoscale, 2022, ⟨10.1039/D2NR02395D⟩. ⟨hal-03705956⟩
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