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Conception d'un hydrogel stratifié : application pour l'ingénierie du cartilage

Abstract : The articular cartilage is composed of chondrocytes and of a specific extracellular matrix which are organized depth-dependently. The tissue did not have an efficient self-renewal of defects. The purpose of this study is to build up layer-by-layer a stratified hydrogel by alternating gels and multilayers polyelectrolytes film spraying, in order to obtain a neotissu in vitro to fill lesions. First, the process was validated by observing a good cells viability and matrix synthesis, and stronger mechanical behaviors of sprayed hydrogels compared to molded one. Secondly, after their spraying, mesenchymal stem cells still have a good viability and their differentiation potential. Then, bistratified scaffolds were built up and cultured up to 56 days without layers dissociation and without cells migration between layers. Finally, scaffolds were functionalized by changing biomaterial composition and by applying mechanicals stimulations. Results show us not only that the composition influences the mechanical behavior of the hydrogel, but that the stratification did not affect it. Furthermore, mechanicals stimulations improve stem cells differentiation in function of biomaterials compositions. In conclusion, this study proves not only that we are able to build up stratified scaffold seeded with mesenchymal stem cells which still have their differentiation capability and synthesize matrix, but that mechanical behaviors are improved after the biomaterial spraying and not alter by the stratification. Moreover, mechanical stimulation applied to the scaffold improves the differentiation of mesenchymal stem cells to a chondrogenic phenotype
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https://hal.univ-lorraine.fr/tel-01746274
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Jessica Tritz-Schiavi. Conception d'un hydrogel stratifié : application pour l'ingénierie du cartilage. Médecine humaine et pathologie. Université Henri Poincaré - Nancy 1, 2011. Français. ⟨NNT : 2011NAN10130⟩. ⟨tel-01746274⟩

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