H. Histologie, 44 a, La subsynoviale, vol.45, p.45

I. Les-pathologies-articulaires-inflammatoires and .. , 50 A. Introduction, p.52

.. Métalloprotéinases-et-dégradation-matricielle-du-cartilage, 59 a Contrôle de la régulation des métalloprotéinases61 b.Contrôle de l'activation du pro-enzyme61 c. Contrôle de l'expression des gènes codant les, p.64

T. Régulation-de-l-'expression-du, 95 ii, ARE et AUBP (Binding Protein) Fonction, vol.8787, p.96

G. De-la, P. Au-niveau-protéique, and .. , 97 B. Les biothérapies actuelles : Inhibition de l'expression, IV. Thérapeutiques actuelles et stratégies d'inhibition.103 D. Les triplex forming oligonucleotides, p.106

I. Expérimentation-animale and .. , 117 A. Préparation des animaux, p.117

H. Macro, 120 G. Mode de transfection, p.121

I. Culture-cellulaire and .. , 126 A. Culture de chondrocytes et de synoviocytes de rat, p.126

A. Partir-des-prélèvements-expérimentaux and .. , 137 a. Membranes synoviales, p.137

.. Vérification-de-l-'intégrité-des-arn-totaux-extraits, 137 a. Dosage spectrophotométrique137 b. Migration sur gel d'agarose, p.138

V. Paramètres-biochimiques, .. , C. Dosage, and E. , 144 A Mesure de la biosynthèse des protéoglycannes, ., p.145

T. Du and T. , 154 B. Evaluation de la concentration optimale pour l'inhibition de l'expression, p.155

A. Mise-au-point-de-la-technique-de-transfection-des-cellules-articulaires-humaines and .. , 161 B. Potentiel inhibiteur des oligonucléotides TFO et siRNA sur des synoviocytes et chondrocytes humains, p.162

T. Effet-du, .. Arnm, and .. , 162 a. Inhibition de l'expression en, ., p.164

T. Du, 167 A. Inhibitions parallèles observées sur les synoviocytes et chondrocytes, .169 C. Evolution de la stabilité des ARNm régulés négativement suite à l'inhibition du, p.172

T. Clonage-du-mrna-du, .. Et-tests-de-surexpression, and T. Du, 174 a. Clonage des différentes régions de l'ARNm174 b. Tests de surexpression des différentes régions de l'ARNm du TNF et évolution de la stabilité des messagers des autres médiateurs, p.176

.. Expression-des-médiateurs-en-arnm, 188 a. Dans les membranes synoviales

T. De, Diminution de la taille de l'oedème suite à l'injection préventive

. Respectivement, Nous nous sommes ainsi interressé aux concentrations de ces médiateurs exprimés (Figure 111

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