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Intérêt des hydrogels polysaccharidiques en ingénierie du cartilage

Abstract : Our thesis work aims to promote a good repair process for a focal chondral lesion. This workpackage associates three main axis : the cell component (the chondrocyte), the biomaterial (extracellular matrix) and the biomechanical constraint (mechanotransduction). Each actor plays a main role in the repair process, at least individually, but mostly in synergy. To this end, various techniques have been developed in order to optimize the biomaterial, to stimulate cells, and fmally to characterize the repair tissue. Three-dimensional chondrocyte culture in alginate plus hyaluronate have thus been developed, to underline the favorable influence of cyclic mechanical constraints. Extracellular matrix component synthesis has been assessed with Capillary Zone Electrophoresis. Secondly, a molecular characterization of encapsulated chondrocytes has been performed by using qPCR in hyaline cartilage versus fibrocartilage. Both lineages did not express the same level of cartilage-dedicated genes, either in basal conditions or after mechanical stimulation or transfection (TGF, BMP). Finally, in vivo implantation in the rat knee during a dedicated calibrated lesion in the patella suggest the favorable influence of a good collaboration between encapsulated chondrocytes and extracellular matrix to promote a good cartilage repair and a good biointegration in a biomechanical-constraint zone.
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Submitted on : Thursday, March 29, 2018 - 10:49:10 AM
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Catherine Gérard. Intérêt des hydrogels polysaccharidiques en ingénierie du cartilage. Biotechnologies. Université Henri Poincaré - Nancy 1, 2005. Français. ⟨NNT : 2005NAN11313⟩. ⟨tel-01746993⟩



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