L. Conditions-de-stringence-utilisées-pendant-la and R. .. Liées-principalement-À-la-température-dj, hybridation des amorces (50°C), sont suffisamment élevées pour que la réaction d'amplification soit spécifique. 1.2. Sensibilité de la réaction PCR La sensibilité du test PCR a été évaluée à 3,1 pg d'ARN cible ce qui, par un calcul théorique, correspond à 2,5 10 6 particules virales. Après "nested-PCR", le seuil de sensibilité est de 0,31 fg d'ARN cible ce qui correspond à 250 particules virales. Ces données sont tout à fait en accord avec les valeurs publiées dans la littérature concernant la détection des segments génomiques des virus de la peste équine ou de la BT (tableau XXXV) En effet

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