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Optimisation de modèles de culture 3D pour la différenciation des cellules souches mésenchymateuses : application à la chondrogénèse

Abstract : ln this study, we were interested in the reconstruction of cartilage tissue based on human mesenchymal stem cells (hMSCs). These cells were characterized by phenotype analysis (CD 34- /CD45-/CD166+/CDI66+/CD90+/CD73+), then they were pipetted in 3D culture, rnicromass. We analyzed the typical mesenchymal markers (CDI05, CD166 and CD90) by flow cytometry along 21 days of cultu:e. The differentiation of hMSCs was induced in micromass cultures with a serum-free medium containing TGF-b1 (10ng/ml). We investigated the modification of cell phenotype, analyzed metabolic activity and proliferation. Our experimental results showed a decrease of hMSCs phenotype markers and appearance of eàiracellular matrix. Furthermare, the analysis of cell cycle revealed a decrease of proliferation activity. We have also defined, in the second part of our study, the culture conditions of a biomaterial (alginate gel) used in cartilage tissue engineering. Different viscosities and concentrations of the biomaterial were monitored. CeIl toxicity and cell proliferation were deterrnined. These results allowed us ta define the experimental condition that will be used in future cartilage tissue engineering study, namely in the effect of mechanical compression.
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https://hal.univ-lorraine.fr/tel-01747456
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Submitted on : Thursday, March 29, 2018 - 11:00:27 AM
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Jingwei Yang. Optimisation de modèles de culture 3D pour la différenciation des cellules souches mésenchymateuses : application à la chondrogénèse. Médecine humaine et pathologie. Université Henri Poincaré - Nancy 1, 2006. Français. ⟨NNT : 2006NAN11305⟩. ⟨tel-01747456⟩

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