Etude des interactions entre métaux lourds et champignons ectomycorhiziens : mise en évidence de gènes impliqués dans la réponse au cadmium de "Paxillus involutus"

Abstract : In vitro tolerance test of ectomycorrhizal isolates from heavy metal-polluted and non-contaminated soils revealed strong intra- and interspecific variations. Moreover, there was no relationship between metal tolerance of isolates and metal concentration at the collection site. Molecular mechanisms underlying the response of the fungus Paxillus involutus towards cadmium have been characterized. We combined suppression subtractive hybridization and cDNA macroarrays hybridization to identify genes whose expression was modulated by cadmium. We screened arrayed cDNAs to identify cadmium-responsive genes by using differential hybridization. A cDNA encoding a superoxide dismutase was cloned from a cDNA library, the encoded protein was presumed to be localized in the peroxisomes. Complementation of a E. coli SOD null strain indicated that the cDNA encode a functional SOD. Monitoring SOD transcript, immunoreactive polypeptide and activity suggests that the level of control is post-tanslational.
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Christophe Jacob. Etude des interactions entre métaux lourds et champignons ectomycorhiziens : mise en évidence de gènes impliqués dans la réponse au cadmium de "Paxillus involutus". Sciences agricoles. Université Henri Poincaré - Nancy 1, 2001. Français. ⟨NNT : 2001NAN10184⟩. ⟨tel-01747498⟩

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