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. La-deuxième-hypothèse-Était, 402, pouvant expliquer la faible expression du gène à partir de pBst-gap4. Nous avons donc recherché d'éventuelles séquences promotrices entre les nucléotides 975 et 1.402. Nous avons observé deux signaux possibles, en positions 1.026 -1.063 et 1.193 -1.229 (Fig. 48) Ces signaux se caractérisent par des séquences -10 n'ayant que le TA en 5' et le T en 3' conservés par rapport au consensus et des séquences -35 présentant la séquence TTGA. Cependant, dans chaque signal, ces séquences -10 et -35 sont très éloignées l'une de l'autre, beaucoup plus que ce qui est normalement observé (17 à 18 nucléotides). TI est possible néanmoins que ces deux signaux puissent contribuer à l'expression du gène de la GAPDH dans pBst-gap4, aucun signal de ce type n'étant présent dans la région 1.402 -2.034 précédant la région codante

G. La and . Pgk, efficacité relative des différents promoteurs possibles. Néanmoins, nous pouvons d'ores et déjà conclure que, dans le cas de B.stearothermophilus, le promoteur en amont de l'opéron est situé à au moins 632 nucléotides en amont du codon d'initiation du gène de, Des expériences de transcription in vitro seront nécessaires pour étudier l Nous venons de voir que, tout comme chez Cpasteurianum ou Z.mobilis (Conway et Ingram, 1988.

G. La-structure-en-opéron-des-gènes-de-la and P. De-la, observée chez chacun des trois micro-organismes Gram-étudiés, est vraisemblablement générale à l'ensemble des bactéries Gram-, Une telle organisation peut permettre une co-régulation étroite des deux enzymes. Ceci est à rapprocher du fait que, selon, ces deux enzymes pourraient être associées in vivo en un complexe enzymatique, 1985.

M. , C. Ainsi, and . Shih, avaient constaté que les enzymes de B .stearothennophilus et Tiaquaticus présentaient une homologie plus forte avec les GAPDH chloroplastiques qu'avec les enzymes cytosoliques des mêmes organismes végétaux, à suggérer que les chloroplastes provenaient d'un ancêtre procaryotique symbiotique dont les gènes auraient été transférés vers le noyau de la cellule-hôte végétale au cours de l'évolution, 1986.

. Les-pourcentages-d, homologie observés s'expliquent par l'existence d'amino-acides présents uniquement dans les "S-loop

. Enfin, des informations supplémentaires concernant l'organisation en opéron, mais également la structure et l'expression des gènes de bactéries Gram-et l'évolution phylogénétique des GAPDH bactériennes, pourront être apportées avec l'étude de la GAPDH de S.lactis subsp. diacetylactis, bactérie Gram+ mésophile aérobie

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