Skip to Main content Skip to Navigation
Theses

Desulfovibrio spp. dans la maladie parodontale : Interactions avec les cellules épithéliales KB et activité de l'amoxicilline libre ou complexée sur ces formes extracellulaires et intracellulaires

Abstract : It has been suggested that Desulfovibrio, which are sulfate-reducing anaerobic bacteria, may be involved in periodontitis. We investigated the capacity of Desulfovibrio to invade epithelial cells and induce cytokine secretion from these cells. We showed that Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis were able to invade and to multiply within oral epithelial cells (KB cells). Intracytoplasmic location of both bacteria was confirmed by confocal laser scanning and transmission microscopy. Invasion of these strains involved microtubule but not microfilament polymerization. Infection by Desulfovibrio resulted in an increase of the production of IL-6 and IL-8 by KB cells. The ability of D. desulfuricans and D. fairfieldensis to survive within oral epithelial cells and to modulate the epithelial immune response may contribute to the initiation and progression of periodontal diseases. Desulfovibrio as well as other periodontopathogens may produce [bêta]-lactamases and have the capacity to invade epithelial cells. It has been suggested that the hydrolysis of amoxicillin might be prevented by using an amoxicillin-[bêta]-cyclodextrin ([bêta]CD) complex and that intracellular diffusion of antimicrobial agents might be enhanced after complexation with [bêta]CDs. A stable [1:1] amoxicillin-[bêta]CD complex, characterized by spectroscopic and thermal analysis, did neither improve the activity of amoxicillin against [bêta]-lactamase producing strains nor enhance the intracellular diffusion of this compound.
Complete list of metadata

Cited literature [92 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01748305
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 11:31:10 AM
Last modification on : Friday, February 26, 2021 - 3:24:03 PM
Long-term archiving on: : Friday, September 14, 2018 - 7:30:39 AM

File

SCD_T_2009_0113_BISSON-BOUTELL...
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01748305, version 1

Collections

Citation

Catherine Bisson-Bouteillez. Desulfovibrio spp. dans la maladie parodontale : Interactions avec les cellules épithéliales KB et activité de l'amoxicilline libre ou complexée sur ces formes extracellulaires et intracellulaires. Sciences agricoles. Université Henri Poincaré - Nancy 1, 2009. Français. ⟨NNT : 2009NAN10113⟩. ⟨tel-01748305⟩

Share

Metrics

Record views

130

Files downloads

552