. Finalité-du-produit-testé, Comment choisir le type de test ?

L. Problème-de and . Représentativité, Comment choisir le modèle viral ? 1. L'évaluation de l'activité ATS antivirale : Enjeux et difficultés

L. Problème-de and . Représentativité, Comment choisir le modèle viral ? 1. L'évaluation de l'activité ATS antivirale : Enjeux et difficultés

L. Problème-de and . Représentativité, Comment choisir le modèle viral ? 1. L'évaluation de l'activité ATS antivirale : Enjeux et difficultés

G. T. Enfinpapageorgiou, certaines publications relatent l'utilisation d'une méthode de filtration sur membrane où, dans ce cas, ce sont les virus qui sont retenus par la membrane et l'ATS-D est éliminé. Des investigations complémentaires restent cependant à réaliser, 2000.

L. Ainsi and . Méthode-développée-au-cours-de-notre-Étude, même si l'étape de neutralisation à proprement parler nécessite 1 min

. Le-ph-dépend, dans notre protocole, de deux paramètres principaux : l'eau qui sert de solvant et les gels de Séphadex?

N. La-norme and . En-14476+a1-préconise-d, une façon générale, l'utilisation d'eau exempte de substances toxiques pour les cellules et bi-distillée ou déminéralisée puis stérilisée par autoclave. Si de l'eau dure doit être utilisée, la norme donne la composition de cette dernière en substances minérales

. Néanmoins, autoclavage induisait une acidification que nous avons neutralisée par ajout de bicarbonate de sodium. En effet, les variations de pH dû à l'autoclavage nous donnaient des résultats très aléatoires en matière de titres viraux

. De-la-même-façon, le pH des gels de Séphadex? a été contrôlé pour être à pH neutre. Ces conditions nous ont permis d'avoir des résultats reproductibles

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