Comment choisir le type de test ? ,
Comment choisir le modèle viral ? 1. L'évaluation de l'activité ATS antivirale : Enjeux et difficultés ,
Comment choisir le modèle viral ? 1. L'évaluation de l'activité ATS antivirale : Enjeux et difficultés ,
Comment choisir le modèle viral ? 1. L'évaluation de l'activité ATS antivirale : Enjeux et difficultés ,
certaines publications relatent l'utilisation d'une méthode de filtration sur membrane où, dans ce cas, ce sont les virus qui sont retenus par la membrane et l'ATS-D est éliminé. Des investigations complémentaires restent cependant à réaliser, 2000. ,
même si l'étape de neutralisation à proprement parler nécessite 1 min ,
dans notre protocole, de deux paramètres principaux : l'eau qui sert de solvant et les gels de Séphadex? ,
une façon générale, l'utilisation d'eau exempte de substances toxiques pour les cellules et bi-distillée ou déminéralisée puis stérilisée par autoclave. Si de l'eau dure doit être utilisée, la norme donne la composition de cette dernière en substances minérales ,
autoclavage induisait une acidification que nous avons neutralisée par ajout de bicarbonate de sodium. En effet, les variations de pH dû à l'autoclavage nous donnaient des résultats très aléatoires en matière de titres viraux ,
le pH des gels de Séphadex? a été contrôlé pour être à pH neutre. Ces conditions nous ont permis d'avoir des résultats reproductibles ,
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