Etude des mécanismes moléculaires impliqués dans la formation des racines adventives du peuplier : rôle du facteur de transcription PtAIL1

Abstract : My thesis was part of the european project ENERGYPOPLAR aiming to improve biomass yield of poplar hybrids in a intensive forestry system. Clonal propagation, by use of cuttings, is a very powerful tool in the fast production of poplars. A key step of this process is the adventitious root formation. Therefore, I investigated the rooting behavior of several selected poplar genotypes. Differences in rooting capacity could be shown suggesting the existence of easy-to-root and difficult-to-root genotypes. The main goal of my thesis was to identify key genes involved in the adventitious rooting process in poplar, with particular interest in the role of transcription factors (TFs). An initial genome-wide transcript profiling of the different stages of adventitious root formation in P. trichocarpa was carried-out. Auxin signaling seems to be involved in poplar adventitious root development, since the transcript level of several gene families like PIN, GH3, AUX/IAA, AUX/LAX and ARF were regulated. Further, the AP2/ERF transcription factors family and in particular AIL1 were identified as important factors involved in adventitious rooting. The phenotypic characterization of poplar transgenics lines (35s::AIL1 and RNAi lines) showed differences in the chronology of adventitious root formation and in the number of roots formed. In order to investigate the pathway(s) associated with PtAIL1 expression, transcriptomic analyses of transgenic lines and wild type were performed. Agamous-like and myb transcription factors were identified as possible candidate genes. PtAIL1 is one of the key regulators involved in adventitious root formation in poplar
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Adeline Rigal. Etude des mécanismes moléculaires impliqués dans la formation des racines adventives du peuplier : rôle du facteur de transcription PtAIL1. Biologie végétale. Université de Lorraine, 2012. Français. ⟨NNT : 2012LORR0006⟩. ⟨tel-01749153⟩

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