? 32 ? 2.1. Origine de la lignée cellulaire CHO utilisée pour produire les protéines recombinantes? 32 ? 2.2. Milieux de culture et métabolisme des cellules de mammifère en culture, . ,
? 43 ? 2.2.3.2. La transferrine, l'insuline, l'albumine et le sélénium (TIBS), ., vol.50 ,
? 60 ? 2.4.2.1. Impact de l'environnement, ., p.61 ,
? 115 ? 1.2.1. Constitution des banques de cellules, Décongélation des cellules, vol.116 ,
? 117 ? 1.3.1. Cultures en système statique ,
? 123 ? 2.2, Pyruvate et ??cétoglutarate, vol.124 ,
? 125 ? 3.1. Quantification de la production d'IFN??? 125 ? 3.2. Caractérisation des modifications post?traductionnelles de l Analyse de la macrohétérogénéité de la glycosylation de l'IFN?? par Western?blot, ., vol.128 ,
Projet de Publication : Influence de 3 suppléments (citrate de fer, éthanolamine et pluronic F?68) sur les cinétiques de croissance et de décès cellulaires, du métabolisme et de production de l'IFN?? au cours de cultures discontinues de cellules CHO 320 réalisées avec le milieu RPMI sans sérum ,
? 269 ? 4.4.2.2. Suivis cinétiques de l'activité de l'IFN?? produit au cours de cultures de cellules CHO 320 effectuées avec le milieu ,
Recombinant therapeutic proteins: Production platforms and challenges, Biotechnology Journal, vol.92, issue.1, pp.90-97, 2008. ,
DOI : 10.1016/S0304-4165(98)00126-3
Biotechnology Research Continues to Bolster Arsenal Against Disease with 633 Medicines in Development, 2008. ,
Animal cell cultures: Recent achievements and perspectives in the production of . Lack of fucose on lf od Thesis mammalian cells: current status and future prospects, 1997. ,
Production of recombinant protein therapeutics in cultivated mammalian cells, Nature Biotechnology, vol.22, issue.11, pp.283-291, 2004. ,
DOI : 10.1016/S0167-7306(03)38027-5
-Linked Oligosaccharide Improves Binding to Human Fc??RIII and Antibody-dependent Cellular Toxicity, Journal of Biological Chemistry, vol.63, issue.30, pp.26733-26740, 2002. ,
DOI : 10.1006/abio.1996.0332
Glycosylation of Recombinant Antibody Therapeutics, Biotechnology Progress, vol.267, issue.1, pp.11-16, 2005. ,
DOI : 10.4049/jimmunol.164.8.4178
Darbepoetin alfa has a longer circulating half-life and greater in vivo potency than recombinant human erythropoietin, Experimental Hematology, vol.31, issue.4, pp.290-299, 2003. ,
DOI : 10.1016/S0301-472X(03)00006-7
The structural role of sugars in glycoproteins, Current Opinion in Biotechnology, vol.7, issue.4, pp.409-416, 1996. ,
DOI : 10.1016/S0958-1669(96)80116-9
Recombinant expression systems in the pharmaceutical industry, Applied Microbiology and Biotechnology, vol.62, issue.4, pp.363-372, 2004. ,
DOI : 10.1007/s00253-004-1656-9
Pr uction et glycosylation de l'interféron?gamma humain par des cellules CHO cultivées en bioréacteurs discontinus et perfusés ,
Pluronic Polyol Effects on Human Gingival Fibroblast Attachment and Growth, Journal of Periodontology, vol.64, issue.5, pp.803-809, 2000. ,
DOI : 10.1902/jop.1993.64.8s.792
The fate of Pluronic F-68 in chondrocytes and CHO cells, Biotechnology and Bioengineering, vol.14, issue.5 ,
DOI : 10.1089/ten.2004.10.987
Structure, localization, and control of cell type?spec glycosylation, J Biol Chem, vol.264, pp.17615-17618, 1989. ,
Intracellular Folding of Tissue-type Plasminogen Activator, Journal of Biological Chemistry, vol.36, issue.9, pp.4797-4804, 1995. ,
DOI : 10.1111/j.1399-3011.1990.tb00958.x
Competition between folding and glycosylation in the endoplasmic reticulum, EMBO J, p.19 ,
A modeling framework for the study of protein glycosylation, Biotechnology and Bioengineering, vol.109, issue.1, pp.73-90, 1996. ,
DOI : 10.1007/978-1-4615-8532-9_7
Influence of intracellular nucleotide and nucleotide sugar contents on recombinant interferon-?? glycosylation during batch and fed-batch cultures of CHO cells, Biotechnology and Bioengineering, vol.36, issue.4, pp.721-733, 2008. ,
DOI : 10.1042/bj3030831
URL : https://hal.archives-ouvertes.fr/hal-00379732
Optimal and consistent protein glycosylation in mammalian cell culture, Glycobiology, vol.1425, issue.3, pp.936-949, 2009. ,
DOI : 10.1002/(SICI)1097-0290(19991020)65:2<182::AID-BIT8>3.0.CO;2-D
An in vitro approach for the expression of recombinant proteins in mammalian cells, Biotechnol Appl Biochem, vol.20, pp.157-171, 1994. ,
A new Chinese hamster ovary cell line expressing ??2,6-sialyltransferase used as universal host for the production of human-like sialylated recombinant glycoproteins, Biochimica et Biophysica Acta (BBA) - General Subjects, vol.1474, issue.3, pp.273-282, 2000. ,
DOI : 10.1016/S0304-4165(00)00023-4
Optimization of hybridoma cell growth and monoclonal antibody secretion in chemically defined serum? and protein?free medium, J Immunol Methods, vol.116, pp.67-77, 1989. ,
Surface tension of protein and insoluble lipids at the air?aqueous phase interface, J Am Oil Chem Soc, vol.75, pp.1233-1239, 1998. ,
Hemacytometer Cell Count Distributions: Implications of Non-Poisson Behavior, 560?563. 50. Goergen JL, Ma hybridoma cultures from the measurement of lactate dehydrogenase release, pp.189-195, 1991. ,
DOI : 10.1021/bp00012a600
Fortification of a protein? free cell culture medium with plant peptones improves cultivation and productivity of an interferon?g producing CHO cell line, In Vitro C ,
Mammalian cell biotechnology, 1990. ,
Studies of protective properties of Pluronic on the Hybridoma cell culture, Chin J Biotechnol, vol.11, pp.101-107, 1995. ,
Effect of Pluronic F-68 on the mechanical properties of mammalian cells, Enzyme and Microbial Technology, vol.14, issue.12, pp.980-983, 1992. ,
DOI : 10.1016/0141-0229(92)90081-X
Response of a Mouse Hybridoma Cell Line to Heat Shock, Agitation, and Sparging, Biotechnology Progress, vol.85, issue.4, pp.175-188, 1989. ,
DOI : 10.1038/bjc.1982.148
Development of a serum?free culture medium for the large scale production of recombinant protein from a Ch ,
MAMMALIAN CELLS IN CHEMICALLY DEFINED MEDIA IN SUSPENSION CULTURES, Annals of the New York Academy of Sciences, vol.149, issue.1 Axenic Cultur, pp.143-161, 1966. ,
DOI : 10.1016/0014-4827(63)90012-0
Characterization of a posttranslational fucosylation in the growth factor domain of urinary plasminogen activator., Proceedings of the National Academy of Sciences, vol.88, issue.9, pp.3992-3996, 1991. ,
DOI : 10.1073/pnas.88.9.3992
Medium Development Cell culture technology for pharmaceutical The effect of fatty acids on hybridoma cell growth and antibody Cacan, 1995. ,
Application of a statistical design to the technol Appl Biochem, pp.87-100, 1992. ,
Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris, Current Opinion in Biotechnology, vol.13, issue.4, pp.329-332, 2002. ,
DOI : 10.1016/S0958-1669(02)00330-0
Effect of copper sulfate on performance The gene coding for inte 112, 1995. ,
Optimisation of the cellular metabolism of glycosylation for recombinant proteins produced by Mammalian cell systems. Cytotechnology. 50:57?76. productivity in serum?free cultures165?73. of newly synthesized glycoproteins1?5. optimization of culture medium for recombinant interferon?? production by CHO cells, J Biotechnol. Biochem Biophys Res Commun. Appl Microbiol Biotechnol, vol.39, issue.38, pp.84-90, 2006. ,
The macroheterogeneity of recombinant human interferon?? produced by Chinese?hamster ovary cells is affected by the protein and lipid content of the culture medium, 1995. ,
Guide des analyses spécialisées Heterologous protein expression in the methylotrophic yeast Pichia pastoris 24:45?66. of a serum?free CHO cell culture process and the level of free thiol in the recombinant antibody expressed, FEMS Microbiol Rev Biotechnol Prog, vol.21, pp.550-553, 2000. ,
The protective effect of specific medium additives with respect to bubble rupture, Biotechnology and Bioengineering, vol.14, issue.6, pp.473-480, 1995. ,
DOI : 10.1016/0167-7799(91)90102-N
A serum-free medium for hybridoma cell culture, Cytotechnology, vol.55, issue.6, pp.169-174, 1993. ,
DOI : 10.1271/bbb1961.55.1631
Structure of the Human Transferrin Receptor-Transferrin Complex, Cell, vol.116, issue.4, pp.565-576, 2004. ,
DOI : 10.1016/S0092-8674(04)00130-8
Abstract, Zeitschrift f??r Naturforschung C, vol.58, issue.7-8, pp.558-561, 2003. ,
DOI : 10.1515/znc-2003-7-819
Identification of cell culture conditions to coproteins expressed in CHO cells, 2009. ,
Characterization of changes in the glycosylation pattern of recombinant proteins from BHK?21 cells due to Geauge Engasser, Influence of the culture oxygenation on the release of LDH by hybridoma cells, Biotechnol Tech, vol.4, pp.257-262, 1990. ,
The fate of Pluronic F?68 in chondrocytes, 2008. ,
Physiological responses Goerge 2) Comparison of specific rates of hybridoma growth and metabolism in batch and continuous cultures, Cytotechnology, vol.10, pp.147-155, 2009. ,
Transmissible spongiform encephalopathies and tissue cell culture. Cytotechnology. 39:117?124. and free radicals generation: is citric acid an innocent additive in foods and drinks? Biometals, pp.793-796, 2002. ,
Ser acceptor sites: implications for protein engineering Protein Eng. 3:433?442. polypeptide integrity and N?glycosylation of a recombinant model glycoprotein 46:536?544. control N?glycosylation site?occupancy of recombinant gly ek Wagne different culture conditions, Biotechnol Bioeng J Biotechnol, vol.42 ,
High-level expression of foreign genes in Hansenula polymorpha, Biotechnology Advances, vol.10, issue.2, pp.179-189, 1992. ,
DOI : 10.1016/0734-9750(92)90002-Q
Agrobacterium-Mediated Plant Transformation: the Biology behind the "Gene-Jockeying" Tool, Microbiology and Molecular Biology Reviews, vol.67, issue.1, pp.16-37, 2003. ,
DOI : 10.1128/MMBR.67.1.16-37.2003
Rapid high?yield expression of full?size IgG antibodies in plants coinfected with noncompeting viral vectros, Biotechnol Bioeng. Proc Nat Acad Sci U.S.A, vol.100, issue.103, pp.975-987, 2006. ,
Cell metabolism Cell culture technology for pharmaceutical and cell?based therapies of CHO cells to repetitive hydrodynamic stress, Biotechnol Bioeng, vol.103, 0199. ,
Determination of cell lysis and death kinetics in continuous hybridoma cultures from the measurement of lactate dehydrogenase release, Cytotechnology, vol.53, issue.3, pp.189-195, 1993. ,
DOI : 10.1007/BF00749869
Monitoring proteolysis of recombinant human interferon?gamma during batch culture of Chinese hamster ovary cells, Cytotechnology, 1997. ,
Monitoring recombinant human interferon?gamma N?glycosylation during perfused fluidized?bed and Gonias Cis?dichlorodiammineplatinum (II), 1988. ,
Environmental Effects on Protein Glycosylation, Nature Biotechnology, vol.258, issue.5, 1990. ,
DOI : 10.1146/annurev.cb.03.110187.001331
Apoptosis in batch cultures of Chinese Hamster Ovary cells, Biotechnology and Bioengineering, vol.47, issue.6, pp.632-640, 1999. ,
DOI : 10.1002/bit.260470209
Improvement of interferon?gamma sialylation in Chinese hamster ovary, 1998. ,
Influence of primatone RL supplementation on ol Chem Ja stirred?tank batch culture of CHO cells 60:596?607. as a selective modifier of the oxidation?sensitive reactive?center methionine in alpha 1? antitrypsin, Biotechnol Bioeng J Biol Chem, vol.363, issue.263, pp.393-397, 1997. ,
Bioprocess Factors Affecting Oligosaccharide Structure and their Effect on Glycoprotein Properties, Nat Biotech, vol.9, pp.1347-1355 ,
Expression of human immune interferon cDNA in E.coli and monkey cells, Nature, vol.295, pp.503-508, 1982. ,
Congenital disorders of glycosylation : a review. Pediatr Res. 52:618?624. cell culture by feeding of N?acetylmannosamine 58:642?648. sialylation of recombinant human interferon?? produced by Chinese hamster ovary cell culture using serum?free media, Biotechnol Bioeng. Biotechnol Bioeng, vol.56, pp.353-360, 2002. ,
Structural Relationship Between Human High and Low Molecular Mass Urokinase, Hoppe-Seyler??s Zeitschrift f??r physiologische Chemie, vol.26, issue.1, 1982. ,
DOI : 10.1021/bi00871a003
Physical and chemical studies on ceruloplasmin. V. Metabolic studies on sialic acid?free ceruloplasmin in vivo, J Biol Chem, vol.243, pp.155-159, 1968. ,
The use of mass spectrometry for the proteomic analysis of glycosylation, PROTEOMICS, vol.77, issue.14, pp.3993-4015, 2006. ,
DOI : 10.1155/2001/963023
URL : https://hal.archives-ouvertes.fr/hal-00087042
Non?fucosylated therapeutic antibodies: the next, Chinese hamster ovary cells to maximize effector Morrow Mortellaro, S. and Devine, M. (2007) Advances in Animal?Free Manufacturing of Biopharmaceuticals, 2004. ,
Structural features of nonionic polyglycol polymer molecules responsible for the protective effect in sparged animal cell bioreactors Effects of buffering conditions and culture pH on production rates and glycosylation of Nachev ova Human interferon gamma: Significance of the C?terminal flexible domain for its biological Nagase Nagira Development of a protein?free medium with iron salts replacing transferrin for a, Biotechnol Muthin M, 1990. ,
Highly efficient transient expression of functional recombinant antibodies in lettuce, Plant Science, vol.169, issue.2, pp.433-438, 2005. ,
DOI : 10.1016/j.plantsci.2005.03.031
Imai?Nishiya, generation of therapeutic antibodies Yamane?Ohnuki, function of produced antibodies using FUT8 siRNA, Cytotechnology. Biotechnol Bioeng, vol.55, issue.88, pp.901-908 ,
D??gradation du cartilage et polyarthrite rhumato??de, Revue du Rhumatisme, vol.72, issue.4, pp.322-330, 2005. ,
DOI : 10.1016/j.rhum.2004.12.021
Natural human interferon?gamma. og, pp.22-30, 1984. ,
Immunogenicity of rDNA?derived pharmaceuticals. Trends uptake and trafficking of Pluronic micelles in drug?sensitive and MDR cells: Effect on the intracellular drug localization, J Pharm Sci, vol.91, p.1, 2002. ,
Quality by design for biopharmaceuticals Evidence that glutamine, not sugar, is the major energy source for cultured HeLa ce, Nat Biotechnol, vol.27, 1979. ,
The Effect of Cell Culture Parameters on Protein Glycosylation, 2002. ,
DOI : 10.1007/0-306-47525-1_2
Eukaryotic glycosylation: whim of nature or multipurpose tool?, Cellular and Molecular Life Sciences (CMLS), vol.55, issue.3, pp.368-422, 1999. ,
DOI : 10.1007/s000180050298
The molecular mechanisms of the metabolism and transport of iron in normal and neoplastic cells, Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, vol.1331, issue.1, pp.1-40, 1997. ,
DOI : 10.1016/S0304-4157(96)00014-7
Degradation of an Fc-fusion recombinant protein by host cell proteases: Identification of a CHO cathepsin D protease, Biotechnology and Bioengineering, vol.265, issue.1, pp.1132-1141, 2009. ,
DOI : 10.1002/bit.22494
Deamidation of Glutaminyl and Asparaginyl Residues in Peptides and Proteins, Curr Top Cell Regul, vol.8, pp.247-295, 1974. ,
DOI : 10.1016/B978-0-12-152808-9.50013-4
Enhanced production of monomeric interferon?beta by CHO cells through the control of culture conditions, Biotechnol Pr ski, vol.23, pp.254-256, 2004. ,
Intracellular human gamma-interferon triggers an antiviral state in transformed murine L cells., Proceedings of the National Academy of Sciences, vol.84, issue.9, pp.2906-2910, 1987. ,
DOI : 10.1073/pnas.84.9.2906
Mapping and partial characterization of proteases expressed by a CHO production cell line, Biotechnology and Bioengineering, vol.113, issue.5, pp.961-971, 2006. ,
DOI : 10.1007/978-94-010-0369-8_52
Effect of glutamine limitation on the death of attached Chinese hamster ovary cells, Biotechnology and Bioengineering, vol.43, issue.1, pp.46-53, 1999. ,
DOI : 10.1002/(SICI)1097-0290(19990705)64:1<46::AID-BIT5>3.0.CO;2-9
Oligosaccharyltransferase: A Complex Multisubunit Enzyme of the Endoplasmic Reticulum, Biochemical and Biophysical Research Communications, vol.266, issue.3, pp.684-689, 1999. ,
DOI : 10.1006/bbrc.1999.1886
Effects of ammonia on CHO cell growth Enhanced erythropoietin heterogeneity in a CHO culture is caused Yoshiro Takashi Cell death caused by selenium deficiency and protective effect of antioxidants, J Biol Chem, vol.278, pp.39428-39434, 2000. ,
36:141?147. utrient deprivation in batch culture, Biotechnol Appl Biochem Biotechnol Bioeng, vol.64, pp.108-119 ,
The Growth Factor Inhibitor Suramin Reduces Apoptosis and Cell Aggregation in Protein-Free CHO Cell Batch Cultures, Biotechnology Progress, vol.16, issue.3, 2000. ,
DOI : 10.1021/bp0000353
Bicarbonate alylation under elevated pCO2 or pH, 182?191. ion, pp.1188-1197, 1999. ,
DOI : 10.1002/(sici)1097-0290(19991020)65:2<182::aid-bit8>3.3.co;2-4
The unfolded protein response: a stress signaling pathway critical Production of human monoclonal antibody in eggs of chimeric chickens, Nat Biotechnol, 2005. ,
To manufacturers of biological products Center for Biologics Evaluation and and glycosylation 68:370?80. by proteolytic degradation and can be eliminated by a high glutamine level, Biotechnol Bioeng Cytotechnology, vol.34, pp.83-99, 2003. ,
Effects of Ammonia and Glucosamine on the Heterogeneity of Erythropoietin Glycoforms, Biotechnology Progress, vol.18, issue.1, pp.129-138, 2002. ,
DOI : 10.1021/bp0101334
Serum protects protein?free competent chinese hamster ovary cells against apoptosis induced by, 319?325. concentration and osmolality are key determinants in the inhibition of CHO cell polysi, 1999. ,
DOI : 10.1002/(sici)1097-0290(19990705)64:1<108::aid-bit12>3.0.co;2-b
A novel function for selenium in biological system: Selenite as a highly effective iron carrier for Chinese hamster ovary cell growth and monoclonal antibody production, Biotechnology and Bioengineering, vol.55, issue.6, 2006. ,
DOI : 10.1002/bit.21081
The unfolded protein response: A stress signaling pathway critical for health and disease, Neurology, vol.66, issue.Issue 1, Supplement 1, pp.102-109, 2005. ,
DOI : 10.1212/01.wnl.0000192306.98198.ec