D. Macinga, S. Sattar, L. Jaykus, and J. Arbogast, Improved Inactivation of Nonenveloped Enteric Viruses and Their Surrogates by a Novel Alcohol-Based Hand Sanitizer, Applied and Environmental Microbiology, vol.74, issue.16, pp.5047-5052, 2008.
DOI : 10.1128/AEM.00487-08

W. Lodder, H. Van-den-berg, S. Rutjes, and A. De-roda-husman, Presence of Enteric Viruses in Source Waters for Drinking Water Production in the Netherlands, Applied and Environmental Microbiology, vol.76, issue.17, pp.5965-5971, 2010.
DOI : 10.1128/AEM.00245-10

K. Valegård, K. Fridborg, and L. Liljas, Crystallization and preliminary X-ray 536, 1994.

C. Dika, M. H. Ly-chatain, G. Francius, J. F. Duval, and C. Gantzer, Non-DLVO adhesion of F-specific RNA bacteriophages to abiotic surfaces: Importance of surface roughness, hydrophobic and electrostatic interactions, Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol.435, 2013.
DOI : 10.1016/j.colsurfa.2013.02.045

URL : https://hal.archives-ouvertes.fr/hal-01114537

D. Taylor and H. Bosmann, Measurement of the electrokinetic properties of vaccinia and reovirus by laser-illuminated whole-particle microelectrophoresis, Journal of Virological Methods, vol.2, issue.5, pp.251-260, 1981.
DOI : 10.1016/0166-0934(81)90023-9

K. Zerda, C. Gerba, K. Hou, and S. Goyal, Adsorption of viruses to charge-modified silica, Appl Environ Microbiol, vol.49, pp.91-95, 1985.

C. Lytle, L. Routson, N. Jain, M. Myers, and B. Green, Virus passage through tracketch membranes modified by salinity and a nonionic surfactant, Appl Environ, vol.65, pp.2773-2775, 1999.

M. Hughes, H. Morgan, and F. Rixon, Measuring the dielectric properties of herpes simplex virus type 1 virions with dielectrophoresis, Biochimica et Biophysica Acta (BBA) - General Subjects, vol.1571, issue.1, pp.1-8, 2002.
DOI : 10.1016/S0304-4165(02)00161-7

C. Schaldach, W. Bourcier, H. Shaw, B. Viani, and W. Wilson, The influence of ionic strength on the interaction of viruses with charged surfaces under environmental conditions, Journal of Colloid and Interface Science, vol.294, issue.1, pp.1-10, 2005.
DOI : 10.1016/j.jcis.2005.06.082

C. Arnal, M. Jaffrezic, S. Oberti, and C. Gantzer, Removal of MS2, Q?, and GA bacteriophages during drinking water treatment at piolot scale, Water Res, vol.46, pp.2651-2664, 2012.

A. Aoyama, R. Hamatake, and M. Hayashi, Morphogenesis of phi X174: in vitro synthesis of infectious phage from purified viral components., Proceedings of the National Academy of Sciences, vol.78, issue.12, pp.7285-7289, 1981.
DOI : 10.1073/pnas.78.12.7285

M. Hayashi, R. Yaghmai, M. Mcconnell, and M. Hayashi, mRNA stabilizing signals encoded in the genome of the bacteriophage ?x174, MGG Molecular & General Genetics, vol.9, issue.2-3, pp.364-371, 1989.
DOI : 10.1007/BF00334377

R. Attinti, J. Wei, K. Kniel, J. Sims, and J. Y. , Virus' (MS2, phiX174, and Aichi) attachment on sand measured by atomic force microscopy and their transport through sand columns, Environ Sci Technol, vol.44, pp.426-432, 2010.

M. Horká, O. Kubícek, F. Ruzicka, V. Holá, I. Malinovská et al., Capillary isoelectric focusing of native and inactivated microorganisms, Journal of Chromatography A, vol.1155, issue.2, pp.164-171, 2007.
DOI : 10.1016/j.chroma.2007.02.026

T. Kinoshita, R. Bales, K. Maguire, and C. Gerba, Effect of pH on bacteriophage transport through sandy soils, Journal of Contaminant Hydrology, vol.14, issue.1, pp.55-70, 1993.
DOI : 10.1016/0169-7722(93)90041-P

J. Schijven and M. Hassanizadeh, Removal of Viruses by Soil Passage: Overview of Modeling, Processes, and Parameters, Critical Reviews in Environmental Science and Technology, vol.30, issue.1, pp.49-127, 2000.
DOI : 10.1080/10643380091184174

J. Schijven, S. Hassanizadeh, and H. De-bruin, Column experiments to study nonlinear removal of bacteriophages by passage through saturated dune sand, Journal of Contaminant Hydrology, vol.58, issue.3-4, pp.243-259, 2002.
DOI : 10.1016/S0169-7722(02)00040-2

J. Bamford and D. Bamford, Capsomer proteins of bacteriophage PRD1, a bacterial virus with a membrane, Virology, vol.177, issue.2, pp.445-451, 1990.
DOI : 10.1016/0042-6822(90)90508-O

D. Alsteens, E. Dague, P. Rouxhet, A. Baulard, and Y. Dufrêne, Direct Measurement of Hydrophobic Forces on Cell Surfaces Using AFM, Langmuir, vol.23, issue.24, pp.11977-11979, 2007.
DOI : 10.1021/la702765c

J. Duval, F. Leermakers, and H. Van-leeuwen, Electrostatic Interactions between Double Layers:?? Influence of Surface Roughness, Regulation, and Chemical Heterogeneities, Langmuir, vol.20, issue.12, pp.5052-5065, 2004.
DOI : 10.1021/la030404f

L. Ogorzaly and C. Gantzer, Development of real-time RT-PCR methods for specific detection of F-specific RNA bacteriophage genogroups: Application to urban raw wastewater, Journal of Virological Methods, vol.138, issue.1-2, pp.131-139, 2006.
DOI : 10.1016/j.jviromet.2006.08.004

M. Mattle, B. Crouzy, M. Brennecke, K. Wigginton, P. Perona et al., Impact of Virus Aggregation on Inactivation by Peracetic Acid and Implications for Other Disinfectants, 2011.

S. Penrod, T. Olson, and S. Grant, Deposition Kinetics of Two Viruses in Packed Beds of Quartz Granular Media, Langmuir, vol.12, issue.23, pp.5576-5587, 1996.
DOI : 10.1021/la950884d

R. Bales, S. Hinkle, T. Kroeger, K. Stocking, and C. Gerba, Bacteriophage adsorption during transport through porous media: chemical perturbations and reversibility, Environmental Science & Technology, vol.25, issue.12, 1991.
DOI : 10.1021/es00024a016

J. Ryan, M. Elimelech, R. Ard, R. Harvey, and P. Johnson, Bacteriophage PRD1 and Silica Colloid Transport and Recovery in an Iron Oxide-Coated Sand Aquifer, Environmental Science & Technology, vol.33, issue.1, pp.63-73, 1999.
DOI : 10.1021/es980350+

J. Zhuang and J. Y. , Interactions between viruses and goethite during saturated flow: Effects of solution pH, carbonate, and phosphate, Journal of Contaminant Hydrology, vol.98, issue.1-2, pp.15-21, 2008.
DOI : 10.1016/j.jconhyd.2008.02.002

J. Loveland, J. Ryan, G. Amy, and R. Harvey, The reversibility of virus attachment to mineral surfaces, Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol.107, pp.205-221, 1996.
DOI : 10.1016/0927-7757(95)03373-4

J. Kyte and R. Doolittle, A simple method for displaying the hydropathic character of a protein, Journal of Molecular Biology, vol.157, issue.1, pp.105-132, 1982.
DOI : 10.1016/0022-2836(82)90515-0

B. Vincent, The Effect of Adsorbed Layers on the Attraction between Colloidal Particles. II. Calculation of Attraction Energy Curves, J Colloid Interface Sci, vol.42, pp.262-269, 1973.

J. Duval, M. J. Anantha, and P. , Electrostatic interactions between diffuse soft multi-layered (bio)particles: beyond Debye???H??ckel approximation and Deryagin formulation, Phys. Chem. Chem. Phys., vol.123, issue.11, 2011.
DOI : 10.1016/0021-9797(88)90230-5

N. Abrescia, J. Cockburn, J. Grimes, G. Sutton, J. Diprose et al., 4e partie Discussion -Conclusion 1 Insights into assembly from structural analysis of bacteriophage PRD1, Phys Chem Chem Phys Nature, vol.13, issue.432, pp.1037-1053, 2004.

H. Ackermann, 5500 Phages examined in the electron microscope, Archives of Virology, vol.79, issue.2, pp.227-243, 2007.
DOI : 10.1007/978-1-4684-5424-6_12

H. Ackermann, Phage Classification and Characterization, Methods in Molecular Biology, vol.501, pp.127-140, 2009.
DOI : 10.1007/978-1-60327-164-6_13

F. Ahimou, M. Paquot, P. Jacques, P. Thonart, and P. Rouxhet, Influence of electrical properties on the evaluation of the surface hydrophobicity of Bacillus subtilis, Journal of Microbiological Methods, vol.45, issue.2, pp.119-126, 2001.
DOI : 10.1016/S0167-7012(01)00240-8

P. Allwood, Y. Malik, C. Hedberg, and S. Goyal, Effect of Temperature and Sanitizers on the Survival of Feline Calicivirus, Escherichia coli, and F-Specific Coliphage MS2 on Leafy Salad Vegetables, Journal of Food Protection, vol.61, issue.7, pp.1451-1456, 2004.
DOI : 10.1128/AEM.69.4.2038-2043.2003

A. Aoyama, R. Hamatake, and M. Hayashi, Morphogenesis of phi X174: in vitro synthesis of infectious phage from purified viral components., Proceedings of the National Academy of Sciences of the, pp.7285-7289, 1981.
DOI : 10.1073/pnas.78.12.7285

R. Aronino, C. Dlugy, E. Arkhangelsky, S. Shandalov, G. Oron et al., Removal of viruses from surface water and secondary effluents by sand filtration, Water Research, vol.43, issue.1, 2009.
DOI : 10.1016/j.watres.2008.10.036

R. Attinti, J. Wei, K. Kniel, J. Sims, and J. Y. , Virus' (MS2, phiX174, and Aichi) attachment on sand measured by atomic force microscopy and their transport through sand columns, Environmental Science & Technology, vol.44, pp.426-432, 2010.

L. Baert, J. Debevere, and M. Uyttendaele, The efficacy of preservation methods to inactivate foodborne viruses, International Journal of Food Microbiology, vol.131, issue.2-3, pp.83-94, 2009.
DOI : 10.1016/j.ijfoodmicro.2009.03.007

R. Bales, S. Li, K. Maguire, M. Yahya, and C. Gerba, MS-2 and poliovirus transport in porous media: Hydrophobic effects and chemical perturbations, Water Resources Research, vol.29, issue.4, pp.957-963, 1993.
DOI : 10.1016/0166-0934(84)90077-6

J. Ball, M. Hardy, R. Atmar, M. Conner, and M. Estes, Oral immunization with recombinant Norwalk virus-like particles induces a systemic and mucosal immune response in mice, Journal of Virology, vol.72, pp.1345-1353, 1998.

R. Baric, B. Yount, L. Lindesmith, P. Harrington, S. Greene et al., Expression and Self-Assembly of Norwalk Virus Capsid Protein from Venezuelan Equine Encephalitis Virus Replicons, Journal of Virology, vol.76, issue.6, pp.3023-3030, 2002.
DOI : 10.1128/JVI.76.6.3023-3030.2002

I. Barrabeig, A. Rovira, J. Buesa, . Bartolome´rbartolome´r, . Pinto´rpinto´r et al., Foodborne norovirus outbreak: the role of an asymptomatic food handler, BMC Infectious Diseases, vol.15, issue.11, p.269, 2010.
DOI : 10.3201/eid1501.080299

S. Benson, J. Bamford, D. Bamford, and R. Burnett, Viral Evolution Revealed by Bacteriophage PRD1 and Human Adenovirus Coat Protein Structures, Cell, vol.98, issue.6, pp.825-833, 1999.
DOI : 10.1016/S0092-8674(00)81516-0

E. Black, C. Guana, D. Kniel, K. Hicks, D. Pivarnik et al., Coliphage as pressure surrogates for enteric viruses in foods, Innovative Food Science & Emerging Technologies, vol.11, issue.2, pp.239-244, 2010.
DOI : 10.1016/j.ifset.2009.07.004

J. Bollback and J. Huelsenbeck, Phylogeny, Genome Evolution, and Host Specificity of Single-Stranded RNA Bacteriophage (Family Leviviridae), Journal of Molecular Evolution, vol.52, issue.2, pp.117-128, 2001.
DOI : 10.1007/s002390010140

N. Boudaud, C. Machinal, F. David, F. Bourdonnec, A. Jossent et al., Removal of MS2, Q?? and GA bacteriophages during drinking water treatment at pilot scale, Water Research, vol.46, issue.8, pp.2651-2664, 2012.
DOI : 10.1016/j.watres.2012.02.020

K. Brorson, H. Shen, S. Lute, J. Pérez, and D. Frey, Characterization and purification of bacteriophages using chromatofocusing, Journal of Chromatography A, vol.1207, issue.1-2, pp.110-121, 2008.
DOI : 10.1016/j.chroma.2008.08.037

G. Bruinsma, M. R. De-vries, J. Busscher, H. Van-der-linden, M. Hooymans et al., Multiple surface properties of worn RGP lenses and adhesion of Pseudomonas aeruginosa, Biomaterials, vol.24, issue.9, pp.1663-1670, 2003.
DOI : 10.1016/S0142-9612(02)00542-2

Y. Buisson and E. Nicand, Vaccination contre l'hépatite A, 2005.

H. Busscher, B. Van-de-belt-gritter, and H. Van-der-mei, Implications of microbial adhesion to hydrocarbons for evaluating cell surface hydrophobicity 1. Zeta potentials of hydrocarbon droplets, Colloids and Surfaces B: Biointerfaces, vol.5, issue.3-4, pp.111-116, 1995.
DOI : 10.1016/0927-7765(95)01224-7

P. Butler, The Current Picture of the Structure and Assembly of Tobacco Mosaic Virus, Journal of General Virology, vol.65, issue.2, pp.253-279, 1984.
DOI : 10.1099/0022-1317-65-2-253

D. Caspar and A. Klug, Physical Principles in the Construction of Regular Viruses, Cold Spring Harbor Symposia on Quantitative Biology, vol.27, issue.0, 1962.
DOI : 10.1101/SQB.1962.027.001.005

S. Chamow, T. Kogan, M. Venuti, T. Gadek, R. Harris et al., Modification of CD4 Immunoadhesin with Monomethoxypoly(Ethylene Glycol) Aldehyde via Reductive Alkylation, Bioconjugate Chemistry, vol.5, issue.2, pp.133-140, 1994.
DOI : 10.1021/bc00026a005

J. Childs and H. Birnboim, Polyacrylamide gel electrophoresis of intact bacteriophage T4D particles, Journal of Virology, vol.16, pp.652-661, 1975.

L. Curtiss and R. Krueger, Localization of coliphage MS2 A-protein, Journal of Virology, vol.14, pp.503-508, 1974.

E. Dague, J. Duval, F. Jorand, F. Thomas, and F. Gaboriaud, Probing Surface Structures of Shewanella spp. by Microelectrophoresis, Biophysical Journal, vol.90, issue.7, pp.2612-2621, 2006.
DOI : 10.1529/biophysj.105.068205

URL : https://hal.archives-ouvertes.fr/hal-00090765

N. Deboosere, A. Pinon, Y. Caudrelier, A. Delobel, G. Merle et al., Adhesion of human pathogenic enteric viruses and surrogate viruses to inert and??vegetal food surfaces, Food Microbiology, vol.32, issue.1, pp.48-56, 2012.
DOI : 10.1016/j.fm.2012.04.007

K. Dingle, P. Lambden, E. Caul, and I. Clarke, Human enteric Caliciviridae: the complete genome sequence and expression of virus-like particles from a genetic group II small round structured virus, Journal of General Virology, vol.76, issue.9, pp.2349-2355, 1995.
DOI : 10.1099/0022-1317-76-9-2349

T. Dokland, R. Bernal, A. Burch, S. Pletnev, B. Fane et al., The role of scaffolding proteins in the assembly of the small, single-stranded DNA virus ??X174 1 1Edited by I. A. Wilson, Journal of Molecular Biology, vol.288, issue.4, pp.595-608, 1999.
DOI : 10.1006/jmbi.1999.2699

H. Douglas, B. Williams, and C. Rondle, Micro-electrophoresis of Pox Viruses in Molar Sucrose, Journal of General Virology, vol.5, issue.3, pp.391-396, 1969.
DOI : 10.1099/0022-1317-5-3-391

S. Dowd, S. Pillai, S. Wang, and M. Corapcioglu, Delineating the specific influence of virus isoelectric point and size on virus adsorption and transport through sandy soils, Applied and Environmental Microbiology, vol.62, pp.405-410, 1998.

J. Duval and H. Ohshima, Electrophoresis of Diffuse Soft Particles, Langmuir, vol.22, issue.8, pp.3533-3546, 2006.
DOI : 10.1021/la0528293

J. Duval and F. Gaboriaud, Progress in electrohydrodynamics of soft microbial particle interphases, Current Opinion in Colloid & Interface Science, vol.15, issue.3, pp.184-195, 2010.
DOI : 10.1016/j.cocis.2009.12.002

J. Duval, M. J. Narayana, and P. , Electrostatic interactions between diffuse soft multi-layered (bio)particles: beyond Debye???H??ckel approximation and Deryagin formulation, Phys. Chem. Chem. Phys., vol.123, issue.11, pp.1037-1053, 2011.
DOI : 10.1016/0021-9797(88)90230-5

M. Estes, J. Ball, R. Guerrero, A. Opekun, M. Gilger et al., Norwalk Virus Vaccines: Challenges and Progress, The Journal of Infectious Diseases, vol.73, issue.s2, pp.367-373, 2000.
DOI : 10.1086/315653

C. Fauquet, M. Mayo, J. Maniloff, U. Desselberger, and L. Ball, Virus taxonomy. Classification and nomenclature of viruses. Eighth report of the International Committee on taxonomy of viruses, 2005.

Y. Feng, S. Ong, J. Hu, X. Tan, and W. Ng, Effects of pH and temperature on the survival of coliphages MS2 and Q?, Journal of Industrial Microbiology and Biotechnology, vol.30, issue.9, pp.549-552, 2003.
DOI : 10.1007/s10295-003-0080-y

S. Flint, J. Brooks, and P. Bremer, Properties of the stainless steel substrate, influencing the adhesion of thermo-resistant streptococci, Journal of Food Engineering, vol.43, issue.4, pp.235-242, 2000.
DOI : 10.1016/S0260-8774(99)00157-0

R. Floyd and D. Sharp, Viral aggregation: quantitation and kinetics of the aggregation of poliovirus and reovirus, Applied and Environmental Microbiology, vol.35, pp.1079-1083, 1978.

A. Fraisse, S. Temmam, N. Deboosere, L. Guillier, A. Delobel et al., Comparison of chlorine and peroxyacetic-based disinfectant to inactivate Feline calicivirus, Murine norovirus and Hepatitis A virus on lettuce, International Journal of Food Microbiology, vol.151, issue.1, pp.98-104, 2011.
DOI : 10.1016/j.ijfoodmicro.2011.08.011

R. Fujioka and B. Yoneyama, Sunlight inactivation of human enteric viruses and fecal bacteria, Asian Waterqual 2001: IWA Asia-Pacific Regional Conference, 2002.

B. Gassilloud and C. Gantzer, Adhesion-aggregation and inactivation of poliovirus 1, 2005.

C. Gerba, Applied and Theoretical Aspects of Virus Adsorption to Surfaces, Advances in Applied Microbiology, vol.30, pp.133-168, 1984.
DOI : 10.1016/S0065-2164(08)70054-6

E. Gias, S. Nielsen, L. Morgan, and G. Toms, Purification of human respiratory syncytial virus by ultracentrifugation in iodixanol density gradient, Journal of Virological Methods, vol.147, issue.2, pp.328-332, 2008.
DOI : 10.1016/j.jviromet.2007.09.013

R. Golmohammadi, K. Valegard, K. Fridborg, and L. Liljas, The Refined Structure of Bacteriophage MS2 at 2??8 ?? Resolution, Journal of Molecular Biology, vol.234, issue.3, pp.620-639, 1993.
DOI : 10.1006/jmbi.1993.1616

L. Goodridge, C. Goodridge, J. Wu, M. Griffiths, and J. Pawliszyn, Isoelectric Point Determination of Norovirus Virus-like Particles by Capillary Isoelectric Focusing with Whole Column Imaging Detection, Analytical Chemistry, vol.76, issue.1, pp.48-52, 2004.
DOI : 10.1021/ac034848s

A. Grahn, J. Haase, E. Lanka, and D. Bamford, Assembly of a functional phage PRD1 receptor depends on 11 genes of the IncP plasmid mating pair formation complex., Journal of Bacteriology, vol.179, issue.15, pp.4733-4740, 2002.
DOI : 10.1128/jb.179.15.4733-4740.1997

M. Guerrero, J. Noel, D. Mitchell, J. Calva, A. Morrow et al., A prospective study of astrovirus diarrhea of infancy in Mexico City, The Pediatric Infectious Disease Journal, vol.17, issue.8, pp.723-727, 1998.
DOI : 10.1097/00006454-199808000-00012

I. Gust, A vaccine against hepatitis A--at last, Medical Journal of Australia, vol.157, pp.345-346, 1992.

A. Hänninen, D. Bamford, and J. Bamford, Assembly of Membrane-Containing Bacteriophage PRD1 Is Dependent on GroEL and GroES, Virology, vol.227, issue.1, pp.207-210, 1997.
DOI : 10.1006/viro.1996.8308

D. Harper, E. Franco, C. Wheeler, D. Ferris, D. Jenkins et al., Efficacy of a bivalent L1 virus-like particle vaccine in prevention of infection with human papillomavirus types 16 and 18 in young women: a randomised controlled trial, The Lancet, vol.364, issue.9447, pp.1757-1765, 2004.
DOI : 10.1016/S0140-6736(04)17398-4

V. Harwood, A. Levine, T. Scott, V. Chivukula, J. Lukasik et al., Validity of the Indicator Organism Paradigm for Pathogen Reduction in Reclaimed Water and Public Health Protection, Applied and Environmental Microbiology, vol.71, issue.6, pp.3163-3170, 2005.
DOI : 10.1128/AEM.71.6.3163-3170.2005

M. Hayashi, R. Yaghmai, M. Mcconnell, and M. Hayashi, mRNA stabilizing signals encoded in the genome of the bacteriophage ?x174, MGG Molecular & General Genetics, vol.9, issue.2-3, pp.364-371, 1989.
DOI : 10.1007/BF00334377

M. Heisenberg, Formation of defective bacteriophage particles by fr amber mutants, Journal of Molecular Biology, vol.17, issue.1, pp.136-144, 1966.
DOI : 10.1016/S0022-2836(66)80100-6

M. Hermansson, The DLVO theory in microbial adhesion, Colloids and Surfaces B: Biointerfaces, vol.14, issue.1-4, pp.105-119, 1999.
DOI : 10.1016/S0927-7765(99)00029-6

M. Horká, O. Kubícek, F. Ruzicka, V. Holá, I. Malinovská et al., Capillary isoelectric focusing of native and inactivated microorganisms, Journal of Chromatography A, vol.1155, issue.2, pp.164-171, 2007.
DOI : 10.1016/j.chroma.2007.02.026

A. Horman, R. Rimhanen-finne, L. Maunula, V. Bonsdorff, C. Torvela et al., Campylobacter spp., Giardia spp., Cryptosporidium spp., Noroviruses, and Indicator Organisms in Surface Water in Southwestern Finland, 2000-2001, Applied and Environmental Microbiology, vol.70, issue.1, pp.87-95, 2000.
DOI : 10.1128/AEM.70.1.87-95.2004

M. Hughes, H. Morgan, and F. Rixon, Measuring the dielectric properties of herpes simplex virus type 1 virions with dielectrophoresis, Biochimica et Biophysica Acta (BBA) - General Subjects, vol.1571, issue.1, pp.1-8, 2002.
DOI : 10.1016/S0304-4165(02)00161-7

J. Israelachvili, Intermolecular and surface forces, 1992.

S. Jiang, R. Noble, and W. Chu, Human Adenoviruses and Coliphages in Urban Runoff-Impacted Coastal Waters of Southern California, Applied and Environmental Microbiology, vol.67, issue.1, pp.179-184, 2001.
DOI : 10.1128/AEM.67.1.179-184.2001

X. Jiang, M. Wang, D. Graham, and M. Estes, Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein, J Virol, vol.66, pp.6527-6532, 1992.

Y. Jin, Y. Chu, and Y. Li, Virus removal and transport in saturated and unsaturated sand columns, Journal of Contaminant Hydrology, vol.43, issue.2, pp.111-128, 2000.
DOI : 10.1016/S0169-7722(00)00084-X

Y. Jin and M. Flury, Fate and Transport of Viruses in Porous Media, Advances in Agronomy, vol.77, pp.39-102, 2002.
DOI : 10.1016/S0065-2113(02)77013-2

J. Jofre, Is the replication of somatic coliphages in water environments significant?, Journal of Applied Microbiology, vol.82, issue.4, pp.1059-1069, 2009.
DOI : 10.1099/00221287-147-11-3121

J. Johnson and J. Speir, Quasi-equivalent viruses: a paradigm for protein assemblies 1 1Edited by T. Richmond, Journal of Molecular Biology, vol.269, issue.5, pp.665-675, 1997.
DOI : 10.1006/jmbi.1997.1068

J. Katainen, M. Paajanen, E. Ahtola, V. Pore, and J. Lahtinen, Adhesion as an interplay between particle size and surface roughness, Journal of Colloid and Interface Science, vol.304, issue.2, pp.524-529, 2006.
DOI : 10.1016/j.jcis.2006.09.015

P. Knappett, M. Emelko, J. Zhuang, and L. Mckay, Transport and retention of a bacteriophage and microspheres in saturated, angular porous media: Effects of ionic strength and grain size, Water Research, vol.42, issue.16, pp.4368-4378, 2008.
DOI : 10.1016/j.watres.2008.07.041

M. Koopmans and E. Duizer, Foodborne viruses: an emerging problem, International Journal of Food Microbiology, vol.90, issue.1, pp.23-41, 2004.
DOI : 10.1016/S0168-1605(03)00169-7

B. Korant, K. Lonberg-holm, F. Yin, and J. Noble-harvey, Fractionation of biologically active and inactive populations of human rhinovirus type 2, Virology, vol.63, issue.2, pp.384-394, 1975.
DOI : 10.1016/0042-6822(75)90311-6

B. Kos, J. Suskovi?, S. Vukovi?, M. Simpraga, J. Frece et al., M92, Journal of Applied Microbiology, vol.62, issue.6, pp.981-987, 2003.
DOI : 10.1111/j.1365-2672.1987.tb02683.x

I. Kukavica-ibrulj, A. Darveau, J. Jean, and I. Fliss, Hepatitis A virus attachment to agri-food surfaces using immunological, virological and thermodynamic assays, Journal of Applied Microbiology, vol.44, issue.5, pp.923-934, 2004.
DOI : 10.1111/j.1365-2672.1984.tb02366.x

J. Langlet, F. Gaboriaud, C. Gantzer, and J. Duval, Impact of Chemical and Structural Anisotropy on the Electrophoretic Mobility of Spherical Soft Multilayer Particles: The Case of Bacteriophage MS2, Biophysical Journal, vol.94, issue.8, pp.3293-3312, 2008.
DOI : 10.1529/biophysj.107.115477

J. Langlet, F. Gaboriaud, J. Duval, and C. Gantzer, Aggregation and surface properties of F-specific RNA phages: Implication for membrane filtration processes, Water Research, vol.42, issue.10-11, pp.2769-2777, 2008.
DOI : 10.1016/j.watres.2008.02.007

J. Langlet, Propriétés interfacailes des bactériophages ARN F-spécifiques: Implication lors des processus d'adhésion-agrégation, Thèse, 2008.

D. Legendre and J. Fastrez, Production in Saccharomyces cerevisiae of MS2 virus-like particles packaging functional heterologous mRNAs, Journal of Biotechnology, vol.117, issue.2, pp.183-194, 2005.
DOI : 10.1016/j.jbiotec.2005.01.010

S. Lima, A. Vaz, T. Souza, D. Peabody, J. Silva et al., Dissecting the role of protein-protein and protein-nucleic acid interactions in MS2 bacteriophage stability, FEBS Journal, vol.20, issue.7, pp.1463-1475, 2006.
DOI : 10.1006/jmbi.2001.5132

M. Lin, H. Lindsay, D. Weitz, R. Ball, R. Klein et al., Universality in colloid aggregation, Nature, vol.339, issue.6223, pp.360-362, 1989.
DOI : 10.1038/339360a0

J. Loveland, J. Ryan, G. Amy, and R. Harvey, The reversibility of virus attachment to mineral surfaces, Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol.107, pp.205-221, 1996.
DOI : 10.1016/0927-7757(95)03373-4

J. Lukasik, T. Scott, D. Andryshak, and S. Farrah, Influence of Salts on Virus Adsorption to Microporous Filters, Applied and Environmental Microbiology, vol.66, issue.7, pp.2914-2920, 2000.
DOI : 10.1128/AEM.66.7.2914-2920.2000

C. Lytle and L. Routson, Minimized virus binding for tests of barrier materials, Applied and Environmental Microbiology, vol.61, pp.643-649, 1995.

C. Lytle, L. Routson, N. Jain, M. Myers, and B. Green, Virus passage through track-etch membranes modified by salinity and a nonionic surfactant, Applied and Environmental Microbiology, vol.65, pp.2773-2775, 1999.

V. Mackiewicz, A. Roque-afonso, and E. Dussaix, HépatiteA: De l'évidence au piège diagnostique, Revue Française des Laboratoires, pp.51-56, 2006.

G. Malescio, Intermolecular potentials ??? past, present, future, Nature Materials, vol.304, issue.8, pp.501-503, 2003.
DOI : 10.1016/S0378-4371(01)00566-0

B. Mandel, Characterization of type 1 poliovirus by electrophoretic analysis, Virology, vol.44, issue.3, pp.554-568, 1971.
DOI : 10.1016/0042-6822(71)90369-2

R. Mastico, S. Talbot, and P. Stockley, Multiple presentation of foreign peptides on the surface of an RNA-free spherical bacteriophage capsid, Journal of General Virology, vol.74, issue.4, pp.541-548, 1993.
DOI : 10.1099/0022-1317-74-4-541

L. Maunula, I. Miettinen, and C. Bonsdorff, Norovirus Outbreaks from Drinking Water, Emerging Infectious Diseases, vol.11, issue.11, pp.1716-1721, 2005.
DOI : 10.3201/eid1111.050487

L. Maunula, M. Roivainen, M. Keränen, S. Mäkelä, K. Söderberg et al., Detection of human Norovirus from frozen raspberries in a cluster of gastroenteritis outbreaks, Euro Surveillance, vol.14, 2009.

L. Mckay, A. Harton, and G. Wilson, Influence of Flow Rate on Transport of Bacteriophage in Shale Saprolite, Journal of Environment Quality, vol.31, issue.4, pp.1095-1105, 2002.
DOI : 10.2134/jeq2002.1095

B. Michen and T. Graule, Isoelectric points of viruses, Journal of Applied Microbiology, vol.109, pp.388-397, 2010.

T. Mix, The physical chemistry of membrane?virus interaction, Developments in Industrial Microbiology, vol.15, pp.136-142, 1974.

L. Mouillot and R. Netter, Identification of Orthopox virus by isoelectrofocusing in a granulated gel, Annales de Microbiologie, vol.128, pp.417-419, 1977.

J. Murray and G. Parks, Poliovirus Adsorption on Oxide Surfaces, 1980.
DOI : 10.1021/ba-1980-0189.ch005

T. Nguyen, N. Easter, L. Gutierrez, L. Huyett, E. Defnet et al., The RNA core weakly influences the interactions of the bacteriophage MS2 at key environmental interfaces, Soft Matter, vol.91, issue.3, pp.10449-10456, 2011.
DOI : 10.1016/S0001-8686(00)00077-4

E. Nicand, C. Bigaillon, and S. Tessé, H??patite E??: maladie ??mergente???, Pathologie Biologie, vol.57, issue.2, pp.203-211, 2008.
DOI : 10.1016/j.patbio.2008.04.002

B. Ninham, On progress in forces since the DLVO theory, Advances in Colloid and Interface Science, vol.83, issue.1-3, pp.1-17, 1999.
DOI : 10.1016/S0001-8686(99)00008-1

O. Callaghan, R. Bradley, R. Paranchych, and W. , Controlled alterations in the physical and biological properties of R17 bacteriophage induced by guanidine hydrochloride, Virology, vol.54, issue.2, pp.476-494, 1973.
DOI : 10.1016/0042-6822(73)90158-X

H. Ohshima, Electrophoresis of soft particles, Advances in Colloid and Interface Science, vol.62, issue.2-3, pp.189-235, 1995.
DOI : 10.1016/0001-8686(95)00279-Y

L. Overby, G. Barlow, R. Doi, M. Jacob, and S. Spiegelman, Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of the viral particles, Journal of Bacteriology, vol.91, pp.442-448, 1966.

J. Paul and C. Kellogg, Ecology of Bacteriophages in Nature. Viral Ecology, pp.211-246, 2000.

D. Peabody, B. Manifold-wheeler, A. Medford, S. Jordan, J. Do-carmo-caldeira et al., Immunogenic Display of Diverse Peptides on Virus-like Particles of RNA Phage MS2, Journal of Molecular Biology, vol.380, issue.1, pp.252-263, 2008.
DOI : 10.1016/j.jmb.2008.04.049

S. Penrod, T. Olson, and S. Grant, Whole Particle Microelectrophoresis for Small Viruses, Journal of Colloid and Interface Science, vol.173, issue.2, pp.521-523, 1995.
DOI : 10.1006/jcis.1995.1354

S. Penrod, T. Olson, and S. Grant, Deposition Kinetics of Two Viruses in Packed Beds of Quartz Granular Media, Langmuir, vol.12, issue.23, pp.5576-5587, 1996.
DOI : 10.1021/la950884d

M. Pham, E. Mintz, and T. Nguyen, Deposition kinetics of bacteriophage MS2 to natural organic matter: Role of divalent cations, Journal of Colloid and Interface Science, vol.338, issue.1, pp.1-9, 2009.
DOI : 10.1016/j.jcis.2009.06.025

G. Pickett and D. Peabody, Encapsidation of heterologous RNAs by bacteriophage MS2 coat protein, Nucleic Acids Research, vol.21, issue.19, pp.4621-4626, 1993.
DOI : 10.1093/nar/21.19.4621

B. Prasad, M. Hardy, T. Dokland, J. Bella, M. Rossmann et al., X-ray Crystallographic Structure of the Norwalk Virus Capsid, Science, vol.286, issue.5438, pp.287-290, 1999.
DOI : 10.1126/science.286.5438.287

C. Priano, R. Arora, J. Butke, and D. Mills, A complete plasmid-based complementation system for RNA coliphage Q??: Three proteins of bacteriophages Q?? (Group III) and SP (Group IV) can be interchanged, Journal of Molecular Biology, vol.249, issue.2, pp.283-297, 1995.
DOI : 10.1006/jmbi.1995.0297

R. Purcell and S. Emerson, Hepatitis E: An emerging awareness of an old disease, Journal of Hepatology, vol.48, issue.3, pp.494-503, 2008.
DOI : 10.1016/j.jhep.2007.12.008

J. Redman, S. Grant, T. Olson, M. Hardy, and M. Estes, Filtration of Recombinant Norwalk Virus Particles and Bacteriophage MS2 in Quartz Sand:?? Importance of Electrostatic Interactions, Environmental Science & Technology, vol.31, issue.12, pp.3378-3383, 1997.
DOI : 10.1021/es961071u

M. Roberts, M. Bentley, and J. Harris, Chemistry for peptide and protein PEGylation, Advanced Drug Delivery Reviews, vol.54, issue.4, pp.459-476, 2002.
DOI : 10.1016/S0169-409X(02)00022-4

M. Rossmann, C. Abad-zapatero, J. Erickson, and H. Savithri, RNA-Protein Interactions in Some Small Plant Viruses, Journal of Biomolecular Structure and Dynamics, vol.266, issue.2, pp.565-579, 1989.
DOI : 10.1038/266417a0

R. Salo and H. Mayor, Isoelectric Focusing of Parvoviruses, Intervirology, vol.10, issue.2, pp.87-93, 1978.
DOI : 10.1159/000148972

L. Santi, Z. Huang, and H. Mason, Virus-like particles production in green plants, Methods, vol.40, issue.1, pp.66-76, 1996.
DOI : 10.1016/j.ymeth.2006.05.020

H. Savilahti and D. Bamford, Protein-primed DNA replication: Role of inverted terminal repeats in the Escherichia coli bacteriophage PRD1 life cycle, Journal of Virology, vol.67, pp.4696-4703, 1993.

C. Schaldach, W. Bourcier, H. Shaw, B. Viani, and W. Wilson, The influence of ionic strength on the interaction of viruses with charged surfaces under environmental conditions, Journal of Colloid and Interface Science, vol.294, issue.1, pp.1-10, 2006.
DOI : 10.1016/j.jcis.2005.06.082

M. Schaper and J. Jofre, Comparison of methods for detecting genotypes of F-specific RNA bacteriophages and fingerprinting the origin of faecal pollution in water samples, Journal of Virological Methods, vol.89, issue.1-2, pp.1-10, 2000.
DOI : 10.1016/S0166-0934(00)00171-3

J. Schijven and M. Hassanizadeh, Removal of Viruses by Soil Passage: Overview of Modeling, Processes, and Parameters, Critical Reviews in Environmental Science and Technology, vol.30, issue.1, pp.49-127, 2000.
DOI : 10.1080/10643380091184174

J. Schijven, S. Hassanizadeh, and H. De-bruin, Column experiments to study nonlinear removal of bacteriophages by passage through saturated dune sand, Journal of Contaminant Hydrology, vol.58, issue.3-4, pp.243-259, 2002.
DOI : 10.1016/S0169-7722(02)00040-2

L. Schwartzbrod, Virus et milieu hydrique, Virologie des milieux hydriques, 1991.

K. Shellenberger and B. Logan, Effect of Molecular Scale Roughness of Glass Beads on Colloidal and Bacterial Deposition, Environmental Science & Technology, vol.36, issue.2, pp.184-189, 2002.
DOI : 10.1021/es015515k

T. Shiba and Y. Suzuki, Localization of A protein in the RNA-A protein complex of RNA phage MS2, Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, vol.654, issue.2, pp.249-255, 1981.
DOI : 10.1016/0005-2787(81)90179-9

P. Shields and S. Farrah, Influence of salts on electrostatic interactions between poliovirus and membrane filters, Applied and Environmental Microbiology, vol.45, pp.526-531, 1983.

P. Shields and S. Farrah, Characterization of Virus Adsorption by Using DEAE-Sepharose and Octyl-Sepharose, Applied and Environmental Microbiology, vol.68, issue.8, pp.3965-3968, 2002.
DOI : 10.1128/AEM.68.8.3965-3968.2002

N. Shirasaki, T. Matsushita, Y. Matsui, A. Oshiba, and K. Ohno, Estimation of norovirus removal performance in a coagulation???rapid sand filtration process by using recombinant norovirus VLPs, Water Research, vol.44, issue.5, pp.1307-1316, 2009.
DOI : 10.1016/j.watres.2009.10.038

L. Sigg, P. Behra, and W. Stumm, Chimie des milieux aquatiques -Chimie des eaux naturelles et des interfaces dans l'environnement, 2006.

R. Sinclair, E. Jones, and C. Gerba, Viruses in recreational water-borne disease outbreaks: a review, Journal of Applied Microbiology, vol.57, issue.6, pp.1769-1780, 2009.
DOI : 10.1093/infdis/165.4.613

H. Smith, I. Hutchison, . Ca, C. Pfannkoch, and J. Venter, Generating a synthetic genome by whole genome assembly: ??X174 bacteriophage from synthetic oligonucleotides, Proceedings of the National Academy of Sciences, vol.147, issue.4, pp.15440-15445, 2003.
DOI : 10.1016/0378-1119(95)00511-4

M. Sobsey, R. Hall, and R. Hazard, Comparative reductions of hepatitis A virus, enteroviruses and coliphage MS2 in miniature soil columns, Water Science and Technology, vol.31, pp.203-209, 1995.

N. Strömsten, D. Bamford, and J. Bamford, The Unique Vertex of Bacterial Virus PRD1 Is Connected to the Viral Internal Membrane, Journal of Virology, vol.77, issue.11, pp.6314-6321, 2003.
DOI : 10.1128/JVI.77.11.6314-6321.2003

C. Tacket, M. Sztein, G. Losonsky, S. Wasserman, and M. Estes, Humoral, mucosal, and cellular immune responses to oral Norwalk virus-like particles in volunteers, Clinical Immunology, vol.108, issue.3, pp.241-247, 2003.
DOI : 10.1016/S1521-6616(03)00120-7

K. Tars, M. Bundule, K. Fridborg, and L. Liljas, The crystal structure of bacteriophage GA and a comparison of bacteriophages belonging to the major groups of Escherichia coli leviviruses, Journal of Molecular Biology, vol.271, issue.5, pp.759-773, 1997.
DOI : 10.1006/jmbi.1997.1214

C. Tartera and J. Jofre, Bacteriophages active against Bacteroides fragilis in sewagepolluted waters, Applied and Environmental Microbiology, vol.53, pp.1632-1637, 1987.

C. Tartera, J. Jofre, and F. Lucena, in different environmental samples, Environmental Technology Letters, vol.34, issue.5, pp.407-410, 1988.
DOI : 10.1016/0043-1354(74)90039-6

D. Taylor and H. Bosmann, Measurement of the electrokinetic properties of vaccinia and reovirus by laser-illuminated whole-particle microelectrophoresis, Journal of Virological Methods, vol.2, issue.5, pp.251-260, 1981.
DOI : 10.1016/0166-0934(81)90023-9

D. Taylor, R. Moore, and L. Sturman, Influence of pH and electrolyte composition on adsorption of poliovirus by soils and minerals, Applied and Environmental Microbiology, vol.42, pp.976-984, 1981.

A. Thill, Agrégation des particules: structure, dynamique et simulation, 1999.

A. Tollefson, M. Kuppuswamy, E. Shashkova, K. Doronin, and W. Wold, Preparation and titration of CsCl-banded adenovirus stocks, Methods in Molecular Medicine, vol.130, pp.223-235, 2007.

K. Toropova, G. Basnak, R. Twarock, P. Stockley, and N. Ranson, The Three-dimensional Structure of Genomic RNA in Bacteriophage MS2: Implications for Assembly, Journal of Molecular Biology, vol.375, issue.3, pp.824-836, 2008.
DOI : 10.1016/j.jmb.2007.08.067

A. Uchiyama and B. Fane, Identification of an Interacting Coat-External Scaffolding Protein Domain Required for both the Initiation of ??X174 Procapsid Morphogenesis and the Completion of DNA Packaging, Journal of Virology, vol.79, issue.11, pp.6751-6756, 2005.
DOI : 10.1128/JVI.79.11.6751-6756.2005

K. Valegård, K. Fridborg, and L. Liljas, Crystallization and preliminary X-ray diffraction studies of the bacteriophage Q??, Acta Crystallographica Section D Biological Crystallography, vol.50, issue.1, pp.105-109, 1994.
DOI : 10.1107/S0907444993009102

C. Van-oss, Forces interfaciales en milieux aqueux, 1996.

C. Van-oss, Long-range and short-range mechanisms of hydrophobic attraction and hydrophilic repulsion in specific and aspecific interactions, Journal of Molecular Recognition, vol.14, issue.4, pp.177-190, 2003.
DOI : 10.1016/S0927-7765(99)00023-5

E. Van-voorthuizen, N. Ashbolt, and A. Schafer, Role of hydrophobic and electrostatic interactions for initial enteric virus retention by MF membranes, Journal of Membrane Science, vol.194, issue.1, pp.69-79, 2001.
DOI : 10.1016/S0376-7388(01)00522-1

F. Veronese, Peptide and protein PEGylation, Biomaterials, vol.22, issue.5, pp.405-417, 2001.
DOI : 10.1016/S0142-9612(00)00193-9

E. Verwey and J. Overbeek, Theory of the stability of lyophobic colloïds, 1948.

J. Vinjé, S. Oudejans, J. Stewart, M. Sobsey, and S. Long, Molecular Detection and Genotyping of Male-Specific Coliphages by Reverse Transcription-PCR and Reverse Line Blot Hybridization, Applied and Environmental Microbiology, vol.70, issue.10, pp.5996-6004, 2004.
DOI : 10.1128/AEM.70.10.5996-6004.2004

D. Wait and M. Sobsey, Comparative survival of enteric viruses and bacteria in Atlantic Ocean seawater, Water Science and Technology, vol.43, pp.139-142, 2001.

G. Walshe, L. Pang, M. Flury, M. Close, and M. Flintoft, Effects of pH, ionic strength, dissolved organic matter, and flow rate on the co-transport of MS2 bacteriophages with kaolinite in gravel aquifer media, Water Research, vol.44, issue.4, pp.1255-1269, 2010.
DOI : 10.1016/j.watres.2009.11.034

J. Walz, The effect of surface heterogeneities on colloidal forces, Advances in Colloid and Interface Science, vol.74, issue.1-3, pp.119-168, 1998.
DOI : 10.1016/S0001-8686(97)00042-0

R. Ward, Mechanism of poliovirus inactivation by ammonia, Journal of Virology, vol.26, pp.299-305, 1978.

T. Westrell, V. Dusch, S. Ethelberg, J. Harris, M. Hjertqvist et al., Norovirus outbreaks linked to oyster consumption in the United Kingdom, Euro Surveill, vol.15, pp.8-11, 2010.

W. Fao, Viruses in Food Scientific Advice to support risk management activities, p.58, 2008.

M. Wolff, C. Siewert, S. Hansen, R. Faber, and U. Reichl, Purification of cell culture-derived modified vaccinia ankara virus by pseudo-affinity membrane adsorbers and hydrophobic interaction chromatography, Biotechnology and Bioengineering, vol.878, issue.i-vi, pp.312-320, 2010.
DOI : 10.1016/j.ijid.2004.09.002

K. Zerda, Adsorption of viruses to charge-modified silica, Baylor College of Medicine, 1982.

K. Zerda and C. Gerba, Agarose isoelectrofocusing of intact virions, Journal of Virological Methods, vol.9, issue.1, pp.1-6, 1984.
DOI : 10.1016/0166-0934(84)90077-6

K. Zerda, C. Gerba, K. Hou, and S. Goyal, Adsorption of viruses to chargemodified silica, Applied and Environmental Microbiology, vol.49, pp.91-95, 1985.

J. Zhuang and J. Y. , Virus retention and transport through Al-oxide coated sand columns: effects of ionic strength and composition, Journal of Contaminant Hydrology, vol.60, issue.3-4, pp.193-209, 2003.
DOI : 10.1016/S0169-7722(02)00087-6

J. Zhuang and J. Y. , Interactions between viruses and goethite during saturated flow: Effects of solution pH, carbonate, and phosphate, Journal of Contaminant Hydrology, vol.98, issue.1-2, pp.15-21, 2008.
DOI : 10.1016/j.jconhyd.2008.02.002

G. Ziedaite, R. Daugelavi?ius, J. Bamford, and D. Bamford, The Holin Protein of Bacteriophage PRD1 Forms a Pore for Small-Molecule and Endolysin Translocation, Journal of Bacteriology, vol.187, issue.15, pp.5397-5405, 2005.
DOI : 10.1128/JB.187.15.5397-5405.2005

G. Ziedaite, DNA Packaging and Host Cell Lysis: Late Events in Bacteriophage PRD1 Infection, ACADEMIC DISSERTATION, 2008.

A. Zlotnick, R. Aldrich, J. Johnson, P. Ceres, and M. Young, Mechanism of Capsid Assembly for an Icosahedral Plant Virus, Virology, vol.277, issue.2, pp.450-456, 2000.
DOI : 10.1006/viro.2000.0619

C. Dika, J. F. Duval, M. H. Ly-chatain, C. Merlin, and C. Gantzer, Impact of internal RNA on aggregation and electrokinetics of viruses: a comparison between MS2 and corresponding VLPs Applied environmental Microbiology, pp.4939-49488, 2011.

C. Dika, C. Merlin, and C. Gantzer, Influence of internal structures (genome) on surface properties of MS2 phage. SarlorLux symposium, septembre, 2009.

C. Gantzer, C. Dika, and J. F. Duval, Determination of surface properties of viral particles: a challenge to explain their fate in aquatic media. Interface against pollution, 2012.

C. Dika, J. F. Duval, C. Merlin, and C. , Gantzer Impact of genome on aggregation and electrokinetics of MS2 phage, influence of the virus purification protocol. Interface against pollution 2012