Étude expérimentale du couplage entre croissance bactérienne et transport d'un polluant organique en milieu poreux

Abstract : An experimental device was performed for the study of coupling the growth of a Shewanella oneidensis MR-1 bacterial biofilm and the non reactive transport of Brilliant Blue FCF. The biofilm growth was monitored by hydraulic conductivity measurements and by image acquisition with a digital camera. The biofilm volume fraction was estimated through tracer experiments with a macromolecular tracer (i.e., Dextran Blue) as in size-exclusion chromatography or gel filtration chromatography. Then after 29 days of bacterial culture a quasi-homogenous biofilm was grown in the whole flow cell (0,1×0,1×0,05m3) occupying about 50% of void space volume. The influence of biofilm growth on porous media transport properties was evaluated. Conservative tracer experiments with Brilliant Blue FCF run at two hydrodynamic conditions and at two growth steps of biofilm (17 and 29 days) showed the influence of local heterogeneities on transport parameters (i.e., porosity, permeability and hydrodynamic dispersion). Then at 17 days of growth when the biofilm partially covers the porous medium (bottom half of the flow cell) a two-equation model or double-layer model was suitable to characterize the conservative transport. A contrario after 29 days of growth, when the biofilm covers the whole porous medium, a classical fickian model was convenient. Numerical values of longitudinal dispersion coefficient from volume averaging well fitted experimental results
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Tiangoua Koné. Étude expérimentale du couplage entre croissance bactérienne et transport d'un polluant organique en milieu poreux. Autre. Université de Lorraine, 2012. Français. ⟨NNT : 2012LORR0404⟩. ⟨tel-01750081⟩

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