S. Du and V. Par, 11 a. Choix de l'enzyme, p.17
URL : https://hal.archives-ouvertes.fr/medihal-00831898

.. La, 17 ii. Le rapport molaire alcool, .

L. Differentes and M. , 21 a Adsorption physique (physisorption) 22 b Adsorption chimique (chimisorption), p.24

M. Utilises, C. Support-d-'enzyme, and .. , 25 a. Les polymères synthétiques organiques 25 b. Les polymères naturels, Matériaux mésoporeux, vol.26, p.26

.. Effet-de-la-taille-des-pores-sur-l-'immobilisation, 27 ii. Effet de la structure et de la morphologie sur l'immobilisation 27 iii. Effet des propriétés de surface sur l'immobilisation, p.28

W. Du, Y. Xu, D. Liu, and J. Zeng, Comparative study on lipase-catalyzed transformation of soybean oil for biodiesel production with different acyl acceptors, Journal of Molecular Catalysis B: Enzymatic, vol.30, issue.3-4
DOI : 10.1016/j.molcatb.2004.04.004

. B. Catal, . D. Enzym, M. Royon, G. Daz, S. Ellenrieder et al., Enzymatic production of biodiesel from cotton seed oil using t-butanol as a solvent Ultrasound assisted enzyme catalyzed transesterification of waste cooking oil with dimethyl carbonate Enzymatic Synthesis of Fatty Alcohol Esters by Alcoholysis Immobilized Lipase-Catalyzed Ethanolysis of Sunflower Oil in a Solvent-Free Medium Kristinsson Preparation of Highly Purified Concentrates of Eicosapentaenoic Acid and Docosahexaenoic Acid, 18 B. Selmi, D. Thomas, pp.125-129, 1997.

. Tominaga, Conversion of Vegetable Oil to Biodiesel Using Immobilized Candida antarctica

J. Lipase, R. C. Am, R. Rodrigues, . Fernandez-lafuente, M. Holmquist et al., Lipase from Rhizomucor miehei as a biocatalyst in fats and oils modification The Role of Arginines in Stabilizing the Active Open-Lid Conformation of Rhizomucor miehei Lipase Lipases at interfaces: A review, 26 H. Fukuda, A. Kondo, H. Noda, Biodiesel fuel production by transesterification of oils 27 G. Kildiran, S. Yücel, S. Ö.Türkay, In situ alcoholysis of soybean oil, pp.789-793, 1993.

M. A. Dasari, M. J. Goff, G. J. Suppes, S. Zheng, M. Kates et al., Noncatalytic alcoholysis kinetics of soybean oil Acid-catalyzed production of biodiesel from waste frying oil The Effects of Catalyst, Free Fatty Acids, and Water on Transecterification of Beef Tallow Trans A two-step acid-catalyzed process for the production of biodiesel from rice bran oil, 32 B. Hamad, R.O. Lopes de Souza, G. Sapaly,M.G. Carneiro Rocha, P.G. Pries de, pp.189-192, 1998.

A. Sheldon, S. Van-pelt, E. Hudson, J. Magner, B. K. Cooney et al., Methodology for the immobilization of enzymes onto mesoporous materials Immobilization of lipase in ordered mesoporous materials: Effect of textural and structural parameters Immobilization of lipase on silicas. Relevance of textural and interfacial properties on activity and selectivity Ethanol improves lipase immobilization on a hydrophobic support Enzyme immobilization using SBA-15 mesoporous molecular sieves with functionalised surfaces, 41 A. Salis, M. F. Casula, M. S. Bhattacharyya, M. Pinna, V. Solinas, M. Monduzzi, Physical and Chemical Lipase Adsorption on SBA-15: Effect of Different Interactions on Enzyme Loading and Catalytic Performance, pp.6223-6235, 2001.

F. Ryoo, F. Di-renzo, and . Fajula, Synthesis of sponge mesoporous silicas from Chapitre I : Généralités 45

F. Macario, U. Verri, A. Diaz, G. Corma, M. Giordano et al., Pure silica nanoparticles for liposome/lipase system encapsulation: Application in biodiesel production, Catalysis Today, vol.204, issue.204, pp.103-114, 2007.
DOI : 10.1016/j.cattod.2012.07.014

T. Wang, H. Hyeon, H. Park, and . Chang, Crosslinked enzyme aggregates in hierarchicallyordered mesoporous silica : a simple and effective method for enzyme stabilization, 45 A.I. Kallenberg, F. van Rantwijk, A. Sheldon, Immobilization of Penicillin, pp.210-218, 2007.

. Acylase, The Key to Optimum Performance, Fuse, I. Chibata, Studies on continuous enzyme reactions. I, pp.905-926, 2005.

C. Tech, M. Delimitsou, A. Zoumpanioti, H. Xenakis, H. Stamatis et al., Activity and Stability Studies Of Mucor miehei Lipase Immobilized in Novel Microemulsion-based Organogels Esterification Reactions Catalyzed by Lipases in Microemulsions: The Role of Enzyme Localization in Relation to Its Selectivity Progress in enzyme immobilization in ordered mesoporous materials and related applications Catalytic Activity in Organic Solvents and Stability of Immobilized Enzymes Depend on the Pore Size and Surface Characteristics of Mesoporous Silica Cytochrome c immobilization into mesoporous molecular sieves, MCM-41 molecular sieve, pp.39-531, 1993.

J. Fan, J. Lei, L. Wang, C. Yu, B. Tu et al., Rapid and high-capacity immobilization of enzymes based on mesoporous silicas with controlled morphologies

V. Murugesan and M. Hartmann, Adsorption of Lysozyme over Mesoporous Molecular Sieves MCM-41 and SBA-15: Influence of pH and Aluminum Incorporation, Mesoporous Silicas in a Mild Buffer Solution and Lysozyme Adsorption, pp.7255-7262, 2007.

. Phys, R. B. Chem, G. Rodrigues, K. Volpato, M. A. Wada et al., Enzymatic Synthesis of Biodiesel from Transesterification Reactions of Vegetable Oils and Short Chain Alcohols, J, vol.108, pp.7323-7330, 2004.

. Am, . Chem, . V. Soc, E. Calabro, M. G. Ricca et al., Kinetics of enzymatic trans-esterification of glycerides for biodiesel production Effects of the combined use of thermomynes lanuginosus and Rhizomucor miehei lipases for the transesterification and hydrolysis of soybean oil Pure silica nanoparticles for liposome/lipase system encapsulation: Application in biodiesel production Lipase from Rhizomucor miehei as an industrieal biocatalyst in chemical process, Bioprocess Biosyst. Eng. Process Biochem. Catal. Today J. Mol. Catal. B: Enzym. Nature, vol.85, issue.359, pp.925-930, 1992.

F. Petroff, G. D. Schüth, . T. Stucky, M. Tanev, T. J. Chibwe et al., Titanium-containing mesoporous molecular sieves for catalytic oxidation of aromatic compounds Templating of Mesoporous Molecular Sieves by Nonionic Polyethylene Oxide Surfactants The physics and chemistry of sol-gel processing Liquid-crystalline phases as templates for the synthesis of mesoporous silica, Nature Nature Science Nature, vol.368, issue.378, pp.317-321, 1994.

B. F. Firouzi, F. Chmelka, G. D. Schuth, and . Stucky, Organization of Organic Molecules with Inorganic Molecular Species into Nanocomposite Biphase Arrays, Chem. Mater, vol.6, pp.1176-1191, 1994.

. Stucky, Triblock Copolymer Syntheses of Mesoporous Silica with Periodic 50 to 300

J. S. Oliveira, I. Pedersen, F. Grillo, C. Meneau, and . Rochas, Structure of Micelles of a Nonionic Block Copolymer Determined by SANS and SAXS, J. Phys. Chem. B, vol.115, pp.11318-11329, 2011.

. Y. Rochas, V. L. Khodakov, M. Zholobenko, D. Imperor-clerc, and . Durand, Characterization of the Initial Stages of SBA-15 Synthesis by in Situ Time-Resolved Small-Angle X-ray Scattering, Nonionic fluorinated surfactant : Investigation of phase diagram and preparation of ordered mesoporous materials, pp.11330-11344, 2004.

K. Meng, F. S. Lin, S. E. Xiao, B. Rankin, H. J. Tan et al., Well-ordered mesoporous silica prepared by cationic fluorinated surfactant templating Ultralow Temperature Synthesis of Ordered Hexagonal Smaller Supermicroporous Silica Using Semifluorinated Surfactants as Template Effects of acidic and basic hydrolysis catalysts on the photocatalytic activity and microstructures of bimodal mesoporous titania Synthesis of mesoporous SBA-15 having macropores by dualtemplating method Synthesis of Macroporous Minerals with Highly Ordered Three-Dimensional Arrays of Spheroidal Voids, High-Temperature Generalized Synthesis of Stable Ordered Mesoporous Silica-Based Materials by Using Fluorocarbon?Hydrocarbon Surfactant Mixtures 84 H. Zhang , A. I. Cooper, Emulsion-Templated Hierarchically Porous Silica Beads Using Silica Nanoparticles as Building Blocks Sen, G. J. T. Tiddy, J. L. Casci, M. W. Anderson, One-Pot Synthesis of Hierarchically Ordered Porous-Silica Materials with Three Orders of Length Scale, pp.3633-3637, 1998.

M. Silica-monolith, F. Carn, A. Collin, M. F. Achard, H. Deleuze et al., Tuning Macropore Entrance Size for Size-Selective Adsorption of Proteins Inorganic monoliths hierarchically textured via concentrated direct emulsion and micellar templates, Chem. Mater. J. Mater. Chem, vol.23, issue.14, pp.2176-2184, 2004.

C. Brinker, Hydrolysis and condensation of silicates: Effects on structure, Journal of Non-Crystalline Solids, vol.100, issue.1-3
DOI : 10.1016/0022-3093(88)90005-1

. Cryst, . L. Solids, D. L. Pham, F. M. Sedlak, and . Doyle, Dissolution of mesoporous silica supports in aqueous solutions: Implications for mesoporous silica-based water treatment processes, pp.31-50, 1988.

C. P. Guthrie and E. J. Reardon, Metastability of MCM-41 and Al-MCM-41, The Journal of Physical Chemistry A, vol.112, issue.15
DOI : 10.1021/jp710434y

. Sahai, Is Silica Really an Anomalous Oxide? Surface Acidity and Aqueous

M. F. Salis, M. S. Casula, M. Bhattacharyya, V. Pinna, M. Solinas et al., Physical and Chemical Lipase Adsorption on SBA-15: Effect of Different Interactions on Enzyme Loading and Catalytic Performance, ChemCatChem, vol.72, issue.3, pp.445-452, 2002.
DOI : 10.1002/cctc.200900288

B. Dizge, A. Keskinler, and . Tanriseven, Biodiesel production from canola oil by using lipase immobilized onto hydrophobic microporous styrene???divinylbenzene copolymer, Biochemical Engineering Journal, vol.44, issue.2-3, pp.220-225, 2009.
DOI : 10.1016/j.bej.2008.12.008

K. W. Ahn, S. H. Ye, W. H. Chun, H. Rah, and S. G. Kim, Yield and component distribution of biodiesel by methanolysis of soybean oil with lipase-immobilized mesoporous silica, Microporous and Mesoporous Materials, vol.142, issue.1, pp.37-44, 2011.
DOI : 10.1016/j.micromeso.2010.11.008

M. K. Lam and K. T. Lee, Catalytic transesterification of high viscosity crude microalgae lipid to biodiesel: Effect of co-solvent, Fuel Processing Technology, vol.110, p.110, 2013.
DOI : 10.1016/j.fuproc.2012.12.021

I. M. Atadashi, M. K. Aroua, A. R. Aziz, and N. M. Sulaiman, The effects of water on biodiesel production and refining technologies: A review, organic solvent system, pp.3456-3470, 2012.
DOI : 10.1016/j.rser.2012.03.004

. Mol, L. Catal, J. C. Giraldo, and . Moreno-piraján, Lipase supported on mesoporous materials as a catalyst in the synthesis of biodiesel from Persea americana mill oil, J Mol Catal B: Enzymat, vol.56, issue.77, pp.122-125, 2009.

L. Yin, Z. Chen, R. Wang, X. Qu, S. Liu et al., Production of biodiesel by esterification of oleic acid with ethanol over organophosphonic acid-functionalized silica, Increasing stability and productivity of lipase enzyme by encapsulation in porous organic-inorganic system, pp.258-263, 2012.
DOI : 10.1016/j.biortech.2012.01.115

J. Goupy, Introduction aux plans d'expériences, Dunod: Paris, 2001.

R. Fisher, Statistical methods for research workers, 1925.

C. Pierlot, J. Poprawski, M. Catté, J. L. Salager, and J. M. Aubry, Experimental design for the determination of the physicochemical parameters of optimum water-oil-surfactant systems, Polymer International, vol.16, issue.4, pp.614-618, 2003.
DOI : 10.1021/la9905517

F. Nielloud, J. P. Mestres, R. Fortuné, S. Draussin, and G. Marti-mestres, Formulation of oil-in-water submicron emulsions in the dermatological field using experimental design, Polymer International, vol.112, issue.4, pp.610-613, 2003.
DOI : 10.1080/00401706.1986.10488093

R. Rodrigues and M. A. Ayub, Effects of the combined use of Thermomyces lanuginosus and Rhizomucor miehei lipases for the transesterification and hydrolysis of soybean oil, Process Biochemistry, vol.46, issue.3, pp.682-688, 2011.
DOI : 10.1016/j.procbio.2010.11.013

M. G. Jang, D. K. Kim, S. C. Park, J. S. Lee, and S. W. Kim, Biodiesel production from crude canola oil by two-step enzymatic process, Renewable energy, pp.99-104, 2012.

V. Dossat, D. Combes, and A. Marty, Continuous enzymatic transesterification of high oleic sunflower oil in a packed bed reactor: influence of the glycerol production, Enzyme and Microbial Technology, vol.25, issue.3-5, pp.194-200, 1999.
DOI : 10.1016/S0141-0229(99)00026-5

Y. Ohta, T. Yamane, and S. Shimizu, Inhibition and Inactivation of Lipase by Fat Peroxide in the Course of Batch and Continuous Glycerolyses of Fat by Lipase, Agricultural and Biological Chemistry, vol.53, issue.7, pp.1885-1890, 1989.
DOI : 10.1080/00021369.1989.10869565

D. Pirozzi, Improvement of lipase stability in the presence of commercial triglycerides, European Journal of Lipid Science and Technology, vol.105, issue.10, pp.608-613, 2003.
DOI : 10.1002/ejlt.200300818

F. Nomoto, K. Ohtsuka, E. Izumoto, H. R. Fukuda, J. L. Teixeira et al., Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water-containing system without an organic solvent, Lipase-Catalyzed Consecutive Batch Reaction for Production of Steryl Esters from Vegetable Oil Deodorizer Distillates, pp.627-631, 1999.

V. Dossat, D. Combes, and A. Marty, Continuous enzymatic transesterification of high oleic sunflower oil in a packed bed reactor: influence of the glycerol production, Enzyme and Microbial Technology, vol.25, issue.3-5, pp.194-200, 1999.
DOI : 10.1016/S0141-0229(99)00026-5

J. L. Serra, L. C. Landeta, and M. De-renobales, Kinetic properties of soluble and immobilized Candida rugosa lipase, Appl. Biochem. Biotechnol, vol.50, pp.127-136, 1995.

Y. Ohta, T. Yamane, and S. Shimizu, Inhibition and Inactivation of Lipase by Fat Peroxide in the Course of Batch and Continuous Glycerolyses of Fat by Lipase, Agricultural and Biological Chemistry, vol.53, issue.7, pp.1885-1890, 1989.
DOI : 10.1080/00021369.1989.10869565

D. Pirozzi, Improvement of lipase stability in the presence of commercial triglycerides, European Journal of Lipid Science and Technology, vol.105, issue.10, pp.608-613, 2003.
DOI : 10.1002/ejlt.200300818

L. Blin, R. Bleta, J. Ghanbaja, and M. J. Stébé, Fluorinated emulsions: Templates for the direct preparation of macroporous???mesoporous silica with a highly ordered array of large mesopores, Microporous and Mesoporous Materials, vol.94, issue.1-3, pp.74-80, 2006.
DOI : 10.1016/j.micromeso.2006.03.023

D. J. Imhof and . Pine, Ordered macroporous materials by emulsion templating, Nature, vol.68, issue.6654
DOI : 10.1103/PhysRevLett.68.3801

F. Kleitz, W. Schmidt, and F. Schuth, Evolution of mesoporous materials during the calcination process: structural and chemical behavior, Microporous and Mesoporous Materials, vol.44, issue.45, pp.95-109, 2001.
DOI : 10.1016/S1387-1811(01)00173-1

F. Bérubé and S. Kaliaguine, Calcination and thermal degradation mechanism of triblock copolymer template in SBA-15 materials, Microporous Mesoporous Mater, p.115, 2008.

H. Takahashi, B. Li, T. Sasaki, C. Miyazaki, T. Kajino et al., Catalytic Activity in Organic Solvents and Stability of Immobilized Enzymes Depend on the Pore Size and Surface Characteristics of Mesoporous Silica, Chemistry of Materials, vol.12, issue.11, pp.3301-3305, 2000.
DOI : 10.1021/cm000487a

V. Dossat, D. Combes, and A. Marty, Continuous enzymatic transesterification of high oleic sunflower oil in a packed bed reactor: influence of the glycerol production, Enzyme and Microbial Technology, vol.25, issue.3-5, pp.194-200, 1999.
DOI : 10.1016/S0141-0229(99)00026-5

C. Qi, Y. Chen, J. H. Huang, Q. Z. Jina, and X. G. Wang, Preparation and characterization of catalase-loaded solid lipid nanoparticles based on soybean phosphatidylcholine, Journal of the Science of Food and Agriculture, vol.99, issue.4, pp.787-793, 2012.
DOI : 10.1002/jps.22129