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, Protocole de mesure des teneurs en HAP totaux des terres des bacs lysimétriques
, Le protocole de quantification des teneurs totales des 16 HAP des terres des bacs lysimétriques est différent du protocole défini en Chapitre II afin de correspondre aux méthodes utilisées au début de la mise en place du dispositif, 2009.
, Extraction des HAP totaux
Les cellules ASE sont remplies de la manière suivante : 1 filtre en fibre de verre de 27mm, un filtre en fibre de verre de 21 mm, 1 g de sulfate de sodium (Na 2 SO 4 ) peeeettaatà deà pigeà l'eauà siduellee,à à gà deà Florisil® (permettant de capter les macromolécules indésirablesà età à gà d'hatilloà deà solà seà età oà à àµ, vol.350 ,
, Laà thodeà d'eettaatioà seà faità aaeeà uà laageà aaatoe/dihloothaaeà /à /) à une température de 100 °C
, Les extraits organiques sont ensuite légèrement évaposà sousà fluà d'azoteà à l'aideà d'uà Calipeeà Lifesciences Turbovap® LV puis réunis daasàuàolueà fialàdeààlàd'aaatoittile. La solution est ensuite transférée dans un flacon de 22ml en verre et muni de bouchons en PTFE (polytétrafluoroéthylène)
Dosage des HAP totaux Lesà teeuusà desà à HáPà sotà oteuesà à l'aideà d'ueà ligeà HPLC (High Pressure Liquid Chromatography) uipeàd'une photodiode de détection (Varian). La séparation est effectuée sur une colonne Pursuit 3PAH (Varian) suivant un gradient eau/acétonitrile, 2013. ,
, Effets des changements structuraux sur le statut de la matière organique
Matrice de corrélation de Pearson entre les taux de variation des teneur en COD, en HAP « disponibles » et des proportions des quatre fractions granulodensimétriques pour la modalité gel/dégel (a) ,
, humectation/dessiccation (b) et pour les deux modalités combinées (c) ??
taux de variations des quatre fractions granulométriques > 1000 µm ; 1000-200 µm ,
, Fluorescence 3D-interprétation des spectres de fluorescence
, Figure XI-16 : Variation du pourcentage d'aromaticité en fonction de l'indice de fluorescence des terres après vieillissement
Schématisation de la détermination de l'effet significatif des facteurs sols et traitements (e.g. séquence climatique) au cours d'une ANOVA deux facteurs selon trois cas : effet gouverné par la nature du sol ,