Identification de polyphénols, évaluation de leur activité antioxydante et étude de leurs propriétés biologiques

Abstract : Natural substances from biomass plant have taken advantage of multiple interests in the biotechnology industries both in food, cosmetic and pharmaceutical. Between these compounds are found much of secondary metabolites which are mainly illustrated in therapeutics. Traditional medicines has used for long time plant materials for healing without knowing what had caused their actions, then studies of secondary metabolites are the subject of numerous studies based on in vitro and in vivo cultures of plant tissues. These include phenolic compounds, polyphenols, flavonoids, anthocyanins, tannins ... which are the subject of our study, compounds widely used in therapeutics as vasculoprotective, anti-inflammatory, enzyme inhibitors, antioxidant and anti free radical. Our work has brought the excerpts from six plants (Daniella oliveri, Desmodium adscendens, Ficus capensis, Securidaca longependuculata, Stevia rebaudiana, and Vitex doniana) traditionally used to treat many diseases in various parts of the world (America, Africa, Asia). The methodology developed for the analysis of these extracts has been applied for the determination of root exudates of Miscanthus x giganteus. The combination of some methods of chemical analysis, spectrophotometry (UV, NMR, MS), chromatography (TLC, CC, HPLC, GC-MS) and biology allowed us to evaluate quantitative and qualitative phenolic extracts from these plants materials. Further analysis helped to highlight the antioxidant capacity and anti-radical of these extracts. While biology tests were used for the evaluation of certain properties such as antimicrobial and anti inflammatory. The results of this work have allowed us to conclude that all plant extracts studied has very good antioxidant properties that may allow us to recommend them in biotechnology.
Document type :
Theses
Complete list of metadatas

Cited literature [138 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01752680
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 1:54:17 PM
Last modification on : Monday, April 16, 2018 - 10:42:34 AM

File

Muanda.Francois.Nsemi.SMZ1011....
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01752680, version 1

Collections

Citation

François Muanda Nsemi. Identification de polyphénols, évaluation de leur activité antioxydante et étude de leurs propriétés biologiques. Biologie végétale. Université Paul Verlaine - Metz, 2010. Français. ⟨NNT : 2010METZ011S⟩. ⟨tel-01752680⟩

Share

Metrics

Record views

309

Files downloads

1006