Analyse transcriptomique chez le chêne pédonculé (Quercus robur) au cours de l'établissement de la symbiose ectomycorhizienne avec le basidiomycète Piloderma croceum

Abstract : The ectomycorrhizal symbiosis establishment involves a molecular cross-talk between both partners. During the pre-symbiotic stage of the interaction between pedonculate oak (Quercus robur) and the basidiomycete Piloderma croceum, ectomycorrhizal classical effects (lateral root elongation and production, increase of leaf area) appear prior to hyphal or root structural differentiation. Transcriptome analysis has shown a limited role of auxin on expression regulation of symbiotic related oak gene. Two sequences coding for chitinases were isolated. The transcript level of the first chitinase was upregulated in lateral roots, whereas no significant differential expression was observed in principal roots. The second chitinase was regulated neither in lateral nor in principal roots in presence of Piloderma croceum. To better describe the plant response to inoculation depending of the root type, we followed the regulation of nearly 600 genes. Interestingly, we showed that fungal presence regulates differentially gene expression in lateral roots and in principal roots. Thus, genes are regulated in principal roots, although these one are not directly involved in the symbiosis. Regulated genes in lateral roots at the pre-mycorrhizal stage are similarly regulated in ectomycorrhiza. Among the regulated genes of which functions are known, nearly 40% play a role in defence mechanisms and response to environmental aggressions.
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Theses
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http://docnum.univ-lorraine.fr/prive/SCD_T_2006_0022_FRETTINGER.pdf
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https://hal.univ-lorraine.fr/tel-01754323
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Submitted on : Friday, March 30, 2018 - 9:44:55 AM
Last modification on : Saturday, March 31, 2018 - 1:28:08 AM

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Patrick Frettinger. Analyse transcriptomique chez le chêne pédonculé (Quercus robur) au cours de l'établissement de la symbiose ectomycorhizienne avec le basidiomycète Piloderma croceum. Biologie végétale. Université Henri Poincaré - Nancy 1, 2006. Français. ⟨NNT : 2006NAN10022⟩. ⟨tel-01754323⟩

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