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Etude de la diversité génétique et fonctionnelle de champignons du sol présentant une activité laccasique : mise au point d'outils moléculaires et application à l'étude comparative de sols agricoles et forestiers

Abstract : Soil microorganisms are involved in biogeochemical cycles. Fungal laccases, exoenzymes lacking substrate specificity, promote the degradation and the condensation of a wide diversity of organic components. They likely play a central role in the formation, stabilization, degradation and then in the cycling of soil organic matter (SOM). To investigate the role of these enzymes, we have developed molecular methods based on PCR allowing the in situ monitoring of the distribution and expression profiles of basidiomycetes laccase genes in soils. For all analyzed soils, we showed a positive correlation between quantity of SOM and the observed fungal species diversity. Temperate forest soils are characterized by a wide diversity of fungal species and an important spatial heterogeneity of the fungal communities. The laccase-gene expression analysis underlines that only a fraction of the total genetic potential was expressed and that several differences exist between rhizosphere and bulk soil.
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Patricia Luis. Etude de la diversité génétique et fonctionnelle de champignons du sol présentant une activité laccasique : mise au point d'outils moléculaires et application à l'étude comparative de sols agricoles et forestiers. Sciences agricoles. Université Henri Poincaré - Nancy 1, 2004. Français. ⟨NNT : 2004NAN10030⟩. ⟨tel-01754339⟩

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