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Comparative genomic analysis of Carnobacterium maltaromaticum: Study of diversity and adaptation to different environments

Abstract : Carnobacterium bacteria are present in a wide variety of environments and foods. The objective of this thesis is to deepen the knowledge on C. maltaromaticum (C. m) by a comparative genomic approach. Nine Carnobacterium genomes, including 5 C. m, available on the platform MicroScope were analyzed and compared. C. m DSM20342 MX5 is the largest genome among LAB. Detailed analysis the presence of a high molecular diversity of cell surface proteins in C. m and C. divergens, while the three other Carnobacterium species present only few proteins on their surfaces. This can be related to the ability of these two species to colonize different habitats. Furthermore, C. m strains isolated from various dairy products are characterized by a large genomic diversity. In order to characterize this diversity, genes involved in lactose (Tagatose-6Phosphate pathway, lac genes) and galactose (Leloir pathway, gal genes) metabolic pathways were identified within Carnobacterium genus and detected by PCR in 42 C. m strains. Both pathways are present and organized differently in the C. m strains. The analysis of these genes at the population level shows a spread of the two pathways within the 4 lineages of this population and suggest that the lac and gal genes have evolved in a complex pattern, which reflects the high level of genetic diversity of the population. These genes were characterized in 237 LAB genome and exhibit a high degree of diversity among the different bacterial genera of the LAB group, with a dominance of the presence of the Leloir pathway. This level of genetic diversity is found at the species level for C. m.
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Christelle Iskandar. Comparative genomic analysis of Carnobacterium maltaromaticum: Study of diversity and adaptation to different environments. Food and Nutrition. Université de Lorraine, 2015. English. ⟨NNT : 2015LORR0245⟩. ⟨tel-01754646⟩



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