Studies of frost hardiness tn Chl,aeella ell;ùpsoidea I-Development of frost hardiness of Chl,orella elùi,psoïdea in synchronous culture, Plant & Cell PhUsinl, vol.17, pp.45-458, 1976. ,
Stabilization and inactivation of biological membranes during freezing in the presence of amino-acids, Biacluim. Bùopthgs. Acta, vol.241, pp.578-589 ,
Effect of freezing on biological membranes in vivo and in vitro, pp.23-37, 1978. ,
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Studies of alginate-immobilized marine microalgae, pp.267-279, 1989. ,
Mechanical and chemical ir\iury to thylakoîd membranes during freezing in vitro, Binclmn. BiophUs. Acta, vol.8, issue.2, pp.78-180, 1985. ,
Viability of Blue-green and Green algae after freezing, Phgsinlagïn Plnntari,um, I 6, pp.530-540, 1963. ,
Effect of varying residual moisture content on the viability of lyophilized algae, p.10141015, 0198. ,
L967, Factors affecting the viability of lyophilized algae, GrUoAinbW, vol.4, issue.1, pp.7-23 ,
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19?3, The effect of cold shock on the blue-green alga Arncgstis nid, pp.881-387 ,
The effects of oils and oil components on algae: A reveiw Br, Phgcol. J., I, vol.1, pp.5-142, 1976. ,
Chilling susceptibility of the blue-green alga Ana,cgstis nid'ulnns ' l. Effect of growth temperature, pp.67-74, 1981. ,
Biosensors for monitoring pollutions of aquatic systems??: applications of algal electrodes, Revue des sciences de l'eau, vol.5, issue.3, pp.45-461, 1992. ,
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Cost-analysis of 5 current aquatic ecotoxicological tests, Report EUR. 11348N, Environment and quality of life, Commission of the european Communities, 1987. ,
Cyst-based toxicity tests : I-Apromising new tool for rapid and cost effective toxicity screening of chemicalsand effluents, furnn Joumal for AWI, pp.235-259, 1991. ,
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FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS, The Journal of Cell Biology, vol.53, issue.1, pp.116-126 ,
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A semi-statiqué microplate-based phytotoxicity test, 1994. ,
J', 1 Effects of temderature on the growth constants of Selenastrum capricornutum Jt"';r*{ ;i-inàWïtË Fôîtîiiir, pp.420-422 ,
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Centre des Sciences de I'Environnement ,
Two different cryoprotective additives were prepared in purified water (Milli Q) Tbe first is a simpte 20 % s.rcrose solution; the second is a mixrure of pollvinylpynolidone soludon (PVP, sucrose ssd 6srhenst ,
000 (SIGMA, 9003-398) was first buffered wirh 0.01 M HEPES rsing a back tiuadon rc a pH 7.0 wiù KOH; sucrose (MERCK,7653) ard merhenol (> 98') soludons were then added to give a final concentration of 20 %(wlv) sucrose and 6 ,
Cells were cooled in a minicooler (Nicool LM l0) ar two differenr rares: a) a slow rete (=l.soC i nrin) undl -3OoC, followed by a fast freezing (=10.5 'C / min) unril -80.c b) a fast rate (=10, p.80 ,
CelI viability, after a freezing-thawing eycle, was assaesed by rneasuring algal growth on either liquid or solid medium in triplicates. Liquid (oligo-L.C.) medium was .rsàd and inhibition of growth was estimated according to ISO 8692 (13), using cell counting (Coulter counter ZVf) aft'er 48 and 96 houns of incubation. The agar plate -elhod (8) ,
nMaintenance of microorganisms : A manual of laboratory methods, pp.253-284, 1980. ,
Cryopreservation of Marine Unicellular Algae. II. Induction of Freezing Tolerance, Marine Ecology Progress Series, vol.2, pp.221-224, 1980. ,
DOI : 10.3354/meps002221
Cryopreservation of Marine Unicellular Algae. I. A. Survey of Algae with Regard to Size, Culture, Age, Photosynthetic Activity and Chlorophyll-to-Cell Ratio, Marine Ecology Progress Series, vol.2, pp.157-173, 1980. ,
DOI : 10.3354/meps002157
Cryopreservation of marine diaùom, 1985. ,
lOR T 90.304, nEssais des eaux -Détermination de I'inhibition de croissance de Scercdesmw subspi.can:rs par une substance, 1980. ,
Analysis and improvement of plant cold hardiness, Boca Raton, 1981. ,
in river water, FEMS Microbiology Letters, vol.43, issue.2, pp.143-746, 1984. ,
DOI : 10.1111/j.1574-6968.1984.tb00200.x
The frozen cells, Ciba foundation Sympaeium, pp.97-114, 1970. ,