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, DLS) permet d'estimer la taille d'une particule en l'assimilant à une sphère dotée d'un rayon hydrodynamique. L'ensemble des rayons hydrodynamiques des particules enregistrés donnera alors une distribution globale de la taille de l'objet, le diamètre D médian de l'objet selon la distribution en volume de

, Le rayon lumineux était produit par un laser Helium-Neon avec une puissance de 5 mW à 633 nm. La lumière était détectée à un angle de 90°. L'échantillon était introduit dans une cellule régulée à 25 °C. Tous les échantillons ont été dilués 50 fois avec de l'eau pure avant observation dans le but d'éviter de saturer le détecteur. L'eau avait été filtrée avant utilisation avec des filtres de cellulose de 0,45µm. Les données ont été analysé avec la méthode, La taille des particules a été estimée à l'aide d'un Malvern Zetasizer 3000 HS

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, Dg) possédant des potentiels redox plus bas, de 20 mV à -400 mV. Ces cytochromes variaient aussi au niveau de leur charge à pH neutre, permettant de valider l'importance des forces électrostatiques dans l'assemblage du biocomposite. Les résultats optimaux obtenus avec c 3 DvH et c 7 Da ont montré l'importance du potentiel redox des éléments exogènes pour l'EET. Nous avons ensuite remplacé le cytochrome c par la protamine. Cette protéine non électroactive a permis l'assemblage du biocomposite tout en maintenant les transferts directs d'électrons entre les bactéries et les différents nanomatériaux testés. Les optimisations ont permis d'atteindre des courants cathodiques de plus de 12 A m -2 en présence de 50 mM de fumarate, Résumé Nous avons cherché dans ce travail à élaborer un biofilm artificiel électroactif dans le but de promouvoir les réactions de transfert extracellulaire d'électrons (EET) en reconstituant artificiellement un biofilm en présence de matériaux exogènes. Un matériau composite auto-assemblé constitué de cellules bactériennes